RC DC™ Protein Assay Kit I #5000121

Use the RC DC Protein Assay for samples containing reducing agents and detergents. This assay, based on the DC Protein Assay, is a modified form of the Lowry assay that is both reducing agent compatible (RC) and detergent compatible (DC), whereas the DC Assay is only detergent compatible.

• Linear over a wide range of protein concentration; standard assay, 0.125–1.5 mg/ml and low concentration assay, 5–250 µg/ml
• Read wavelengths, 650–750 nm
• Absorbance values are stable ≥ 1 h
• Quantitate protein in sample loading buffer prior to gel electrophoresis, either 1-D or 2-D format
• Determine protein recovery in cell lysates

Sample components compatible with the RC DC Protein Assay include:

• • Ammonium sulfate, 0.5 M
  • β-mercaptoethanol, 10%*
  • Brij 35, 1%
  • C12E8, 0.2%
  • Calcium chloride, 50 mM
  • CHAPS, 2%
  • CHAPSO 1%
• • Dithiothreitol (DTT), 350 mM*
  • EDTA 0.1 M
  • Guanidine hydrochloride, 0.4 M
  • Imidazole, 0.5 M
  • Laemmli buffer (5% β-mercaptoethanol)
  • Nonidet P-40, 2%
  • Octyl-β-glucoside, 1%
• • ReadyPrep™ Sequential Extraction Reagents 2 and 3*
  • SDS, 10%
  • Tributylphosphine (TBP), 2 mM
  • Tris, pH 8.4, 0.5 M
  • Triton X-100, 2%
  • Tween 20, 2%
  • Urea, 4 M

* Two washes required for highest concentrations

Product Contents

• RC Reagent I, 250 ml
• RC Reagent II, 250 ml
• Alkaline copper tartrate, 250 ml
• Dilute Folin reagent, 2 L
• Surfactant solution, 5 ml
• Lyophilized bovine γ-globulin, 1 vial

Packaging Options

Related Products

• Protein Standard I, lyophilized bovine γ-globulin (5000005)
• Protein Standard II, lyophilized bovine serum albumin (5000007)
• Semimicrovolume Disposable Polystyrene Cuvettes (2239955)
• Standard Disposable Polystyrene Cuvettes (2239950)
• Protein Assay Reagent A, refill, 250 ml alkaline copper tartrate (5000113)
• Protein Assay Reagent B, refill, 1 L Folin reagent (5000114)
• Protein Assay Reagent S, refill, 5 ml surfactant (5000115)
• RC Reagent I, refill, 250 ml (5000117)
• RC Reagent II, refill, 250 ml (5000118)

Related Categories

• SmartSpec™ Plus Spectrophotometer
• Microplate Readers
• Mixing Devices
• Centrifuges
• Protein Assay Kits and Cuvettes

Supporting Documentation

• RC DC Protein Assay Product Information Sheet
• RC DC Protein Assay Instruction Manual
• Colorimetric Protein Assays Application Note

Reference

Lowry OH et al. (1951). Protein Measurement with the Folin Phenol Reagent. J Biol Chem 193, 265-275. PMID: 14907713