Description
Description
Use the RC DC Protein Assay for samples containing reducing agents and detergents. This assay, based on the DC Protein Assay, is a modified form of the Lowry assay that is both reducing agent compatible (RC) and detergent compatible (DC), whereas the DC Assay is only detergent compatible.
- Linear over a wide range of protein concentration; standard assay, 0.125–1.5 mg/ml and low concentration assay, 5–250 µg/ml
- Read wavelengths, 650–750 nm
- Absorbance values are stable ≥ 1 h
- Quantitate protein in sample loading buffer prior to gel electrophoresis, either 1-D or 2-D format
- Determine protein recovery in cell lysates
Sample components compatible with the RC DC Protein Assay include:
-
- Ammonium sulfate, 0.5 M
- β-mercaptoethanol, 10%*
- Brij 35, 1%
- C12E8, 0.2%
- Calcium chloride, 50 mM
- CHAPS, 2%
- CHAPSO 1%
-
- Dithiothreitol (DTT), 350 mM*
- EDTA 0.1 M
- Guanidine hydrochloride, 0.4 M
- Imidazole, 0.5 M
- Laemmli buffer (5% β-mercaptoethanol)
- Nonidet P-40, 2%
- Octyl-β-glucoside, 1%
-
- ReadyPrep™ Sequential Extraction Reagents 2 and 3*
- SDS, 10%
- Tributylphosphine (TBP), 2 mM
- Tris, pH 8.4, 0.5 M
- Triton X-100, 2%
- Tween 20, 2%
- Urea, 4 M
Product Contents
- RC Reagent I, 250 ml
- RC Reagent II, 250 ml
- Alkaline copper tartrate, 250 ml
- Dilute Folin reagent, 2 L
- Surfactant solution, 5 ml
- Lyophilized bovine γ-globulin, 1 vial
Packaging Options
Kit | Protein Standard | Catalog Number |
RC DC Protein Assay Kit I | Bovine γ-globulin | 5000121 |
RC DC Protein Assay Kit II | Bovine serum albumin | 5000122 |
Related Products
- Protein Standard I, lyophilized bovine γ-globulin (5000005)
- Protein Standard II, lyophilized bovine serum albumin (5000007)
- Semimicrovolume Disposable Polystyrene Cuvettes (2239955)
- Standard Disposable Polystyrene Cuvettes (2239950)
- Protein Assay Reagent A, refill, 250 ml alkaline copper tartrate (5000113)
- Protein Assay Reagent B, refill, 1 L Folin reagent (5000114)
- Protein Assay Reagent S, refill, 5 ml surfactant (5000115)
- RC Reagent I, refill, 250 ml (5000117)
- RC Reagent II, refill, 250 ml (5000118)
Related Categories
- SmartSpec™ Plus Spectrophotometer
- Microplate Readers
- Mixing Devices
- Centrifuges
- Protein Assay Kits and Cuvettes
Supporting Documentation
- RC DC Protein Assay Product Information Sheet
- RC DC Protein Assay Instruction Manual
- Colorimetric Protein Assays Application Note
Reference
Lowry OH et al. (1951). Protein Measurement with the Folin Phenol Reagent. J Biol Chem 193, 265-275. PMID: 14907713