Description
Description
Nuvia IMAC Resin is a next-generation affinity chromatography media, with ultra-high capacity over a range of pH and flow rates, that provides a wide experimental design space for many applications.
Nuvia IMAC Resin, based on Nuvia Beads, contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The chemical structure of NTA permits highly selective binding of recombinant histidine-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.
Due to superior mass transfer characteristics, Nuvia IMAC Resin offers high capacity at high flow rates. The resin can be used under either nondenaturing or denaturing conditions.
Features and Benefits of Nuvia IMAC Resin
- Best-in-class binding capacity at fast flow rates
- Rapid mass transfer kinetics
- Efficient resolution of biomolecules from crude feedstreams
- Stable at pH 2–14, providing excellent NaOH stability
- Chelating ligand nitrilotriacetic acid (NTA) may be charged with transition metals such as Cu2+, Ni2+, Co2+, or Zn2+
Applications of Nuvia IMAC Resin
For purifying biomolecules in a variety of workflows:
- Excellent single-step purity via affinity chromatography
- Quick, efficient separation of biomolecules
- Contaminant removal
- Robust base matrix
This IMAC Resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.
Packaging Options
| Package Size | Catalog Number |
| Nuvia IMAC Ni-Charged Support, bulk resin | |
| 25 ml | 7800800 |
| 100 ml | 7800801 |
| 500 ml | 7800802 |
| 5 L | 12003233 |
| 10 L | 12002782 |
| Nuvia IMAC Ni-Charged Support, prepacked | |
| Prepacked column, 1 ml | 12004038 |
| Prepacked column, 5 ml | 12004037 |
| Prepacked filter plates, 20 µl | 12004035 |
| Prepacked RoboColumns, 200 µl | 12004051 |
| Prepacked RoboColumns, 600 µl | 12004052 |
| Nuvia IMAC Uncharged Support, bulk resin | |
| 5 L | 12004040 |
| 10 L | 12004039 |
Prepacked Nuvia IMAC Columns
Use these 5 ml cartridges with the NGC™ Chromatography System or any low- to medium-pressure chromatography system.
More Information
Download the Nuvia IMAC Resin Instruction Manual for detailed column packing recommendations and protocols for optimal protein isolation and column regeneration, cleaning, sanitizing, and storage.
Related Products
View all Affinity Chromatography Resins.
Empty chromatography columns
- Spin columns
- Low-pressure EconoColumn Columns
- Medium-pressure columns
Desalting columns for secondary purification
Ordering
Ordering
items
Use the filters below to refine results!
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system
Pkg of 1, 1 ml, prepacked Ni-charged IMAC column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system
Pkg of 1, 5 ml, prepacked Ni-charged IMAC column, 16 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system
Pkg of 5, 1 ml, prepacked Ni-charged IMAC column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system
Pkg of 5, 5 ml, prepacked Ni-charged IMAC column, 16 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system
Pkg of 1, 1 ml, prepacked uncharged IMAC column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system
