Nuvia IMAC Resin

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Overview

Nuvia IMAC (immobilized metal affinity chromatography) Resin provides an ultra-high capacity matrix over a wide range of flow rates and pH. Get reproducible separation of biomolecules free of contaminants.
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Description

Description

Nuvia IMAC Resin is a next-generation affinity chromatography media, with ultra-high capacity over a range of pH and flow rates, that provides a wide experimental design space for many applications.

Nuvia IMAC Resin, based on Nuvia Beads, contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The chemical structure of NTA permits highly selective binding of recombinant histidine-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.

Due to superior mass transfer characteristics, Nuvia IMAC Resin offers high capacity at high flow rates. The resin can be used under either nondenaturing or denaturing conditions.

Features and Benefits of Nuvia IMAC Resin

  • Best-in-class binding capacity at fast flow rates
  • Rapid mass transfer kinetics
  • Efficient resolution of biomolecules from crude feedstreams
  • Stable at pH 2–14, providing excellent NaOH stability
  • Chelating ligand nitrilotriacetic acid (NTA) may be charged with transition metals such as Cu2+, Ni2+, Co2+, or Zn2+

Applications of Nuvia IMAC Resin

For purifying biomolecules in a variety of workflows:

  • Excellent single-step purity via affinity chromatography
  • Quick, efficient separation of biomolecules
  • Contaminant removal
  • Robust base matrix

This IMAC Resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.

Packaging Options

Package Size Catalog Number
Nuvia IMAC Ni-Charged Support, bulk resin  
25 ml 7800800
100 ml 7800801
500 ml 7800802
5 L 12003233
10 L 12002782
Nuvia IMAC Ni-Charged Support, prepacked  
Prepacked column, 1 ml 12004038
Prepacked column, 5 ml 12004037
Prepacked filter plates, 20 µl 12004035
Prepacked RoboColumns, 200 µl 12004051
Prepacked RoboColumns, 600 µl 12004052
Nuvia IMAC Uncharged Support, bulk resin  
5 L 12004040
10 L 12004039

 

Prepacked Nuvia IMAC Columns
Use these 5 ml cartridges with the NGC™ Chromatography System or any low- to medium-pressure chromatography system.

 

More Information

Download the Nuvia IMAC Resin Instruction Manual for detailed column packing recommendations and protocols for optimal protein isolation and column regeneration, cleaning, sanitizing, and storage.

Related Products

View all Affinity Chromatography Resins.

Empty chromatography columns

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Ordering

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items

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nuvia-imac-nicharged-resin-780-0800-thumb.jpg
7800800
Pkg of 1, 25 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
nuvia-imac-nicharged-resin-780-0801-thumb.jpg
7800801
Pkg of 1, 100 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
nuvia-imac-nicharged-resin-780-0802-thumb.jpg
7800802
Pkg of 1, 500 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
12004040-nuvia-imac-uncharged-resin-5-l-tn.jpg
12003233
Pkg of 1, 5 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
12004039-nuvia-imac-uncharged-resin-10-l-tn.jpg
12002782
Pkg of 1, 10 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
12004040-nuvia-imac-uncharged-resin-5-l-tn.jpg
12004040
Pkg of 1, 5 L, Nuvia IMAC Support with uncharged nitrilotriacetic acid (NTA) for immobilized metal affinity chromatography
12004039-nuvia-imac-uncharged-resin-10-l-tn.jpg
12004039
Pkg of 1, 10 L, Nuvia IMAC Support with uncharged nitrilotriacetic acid (NTA) for immobilized metal affinity chromatography
12004038-foresight-nuvia-ni-charged-imac-1-ml-column-tn.jpg
12004038
Pkg of 1, 1 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin
12004037-foresight-nuvia-ni-charged-imac-5-ml-column-tn.jpg
12004037
Pkg of 1, 5 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin
12004035-foresight-nuvia-ni-charged-imac-filter-plates-20-ul-tn.jpg
12004035
2 x 96-well plates, 20 µl, filter plate prepacked with Nuvia IMAC Affinity Chromatography Resin
12004051-foresight-nuvia-ni-charged-imac-robocolumn-unit-200-ul-tn.jpg
12004051
1 row of 8 columns, 200 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin
12004052-foresight-nuvia-foresight-nuvia-ni-charged-imac-robocolumn-unit-600-ul-tn.jpg
12004052
1 row of 8 columns, 600 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin
bio-scale-mini-nuvia-imac-nicharged-column-780-0811-thumb.jpg
7800811
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system
bio-scale-mini-nuvia-imac-nicharged-columns-780-0812-thumb.jpg
7800812
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system
EconoFit_Columns-1x1ml-Sku_TN.png
12009288

Pkg of 1, 1 ml, prepacked Ni-charged IMAC column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system

EconoFit_Columns-1x5ml-Sku_TN.png
12009286

Pkg of 1, 5 ml, prepacked Ni-charged IMAC column, 16 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system

EconoFit_Columns-5x1ml-Sku_TN.png
12009285

Pkg of 5, 1 ml, prepacked Ni-charged IMAC column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system

EconoFit_Columns-5x5ml-Sku_TN.png
12009287

Pkg of 5, 5 ml, prepacked Ni-charged IMAC column, 16 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system

EconoFit_Columns-1x1ml-Sku_TN.png
12009289

Pkg of 1, 1 ml, prepacked uncharged IMAC column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system

Documents

Documents

Number Description Options
D116698
Instruction Manual, Nuvia™ IMAC Resin
6859
Nuvia™ IMAC Affinity Chromatography Resin Product Information Sheet, Ver B
10041106
Instruction Manual, Bio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges, 1 and 5 ml, Rev A
10042131
Instruction Manual, Bio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges, 1 and 5 ml Quick Start Guide, Rev B
6860
A Scalable Immobilized Metal Affinity Chromatography Resin for Process Purification
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6964
A Nuvia IMAC Resin–Based Purification Workflow for the Production of Highly Pure Recombinant Proteins
6769
From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification (Poster), Ver A
7028
Nuvia IMAC Packing Protocol Application Note, Ver A