Use IEF Standards for pI calibration in analytical polyacrylamide or agarose isoelectric focusing (IEF) gels. Our IEF Standards are a mixture of nine proteins (pI 4.45–9.6) in a ready-to-use aqueous solution. Five of the nine proteins are naturally colored, allowing continuous monitoring of the focusing process.
IEF Standards Specifications
|Size Range||Quantity||Recommended Loading Volume|
|9 proteins, pI 4.45–9.6||16 mg protein in 250 µl of 50% (v/v) glycerol with 0.02% NaN3||Mini gels: 3 µl (Coomassie R-250 stain/crocein scarlet); 0.5 µl (silver stain)|
Supporting Documents and Resources
- IEF Standards Instruction Manual