IEF Standards #1610310



250 μl, pI range: 4.45–9.6, phycocyanin, β-lactoglobulin B, bovine and human CA, equine myoglobin, human hemoglobin A and C, lentil lectin, and cyt C
Safety Data Sheet

Safety Data Sheet



Use IEF Standards for pI calibration in analytical polyacrylamide or agarose isoelectric focusing (IEF) gels. Our IEF Standards are a mixture of nine proteins (pI 4.45–9.6) in a ready-to-use aqueous solution. Five of the nine proteins are naturally colored, allowing continuous monitoring of the focusing process.

IEF Standards Specifications

Size Range Quantity Recommended Loading Volume
9 proteins, pI 4.45–9.6 16 mg protein in 250 µl of 50% (v/v) glycerol with 0.02% NaN3 Mini gels: 3 µl (Coomassie R-250 stain/crocein scarlet); 0.5 µl (silver stain)

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