Overview
The Vericheck ddPCR Replication Competent Lentivirus (RCL) kit provides a rapid, cost effective, sensitive, and fully validated solution for the detection of RCL.
Safety Data Sheet
Description
Description
Lentiviruses are commonly used as viral vectors in cell and gene therapy production, delivering and steadily integrating desired genes into the genome of the host cell. However, they may also present health hazards to humans due to integration-mediated transformation and the creation of a RCL that can infect nontarget cells. While the current regulatory guidelines recommend a cell culture–based method for RCL testing, it is cumbersome and takes 30–45 days from sample to results. Bio-Rad’s Vericheck ddPCR RCL Kit is fast, cost-effective, specific, and a fully validated solution for lentivirus testing.
Features and Benefits
- Fast turnaround time: Results in as little as 8 hours
- High sensitivity: LOD of 0.35 copies/µl
- Reproducible results: Low CV of <10% between lots, users, plates and instruments
- Automated data analysis: Simplified workflow with positive controls included in the kit to aid automated results
- Comprehensive ddPCR workflow: Supports and provides flexibility to run multiple samples per run
Product Contents
| Description | Catalog | Quantity |
| ddPCR RCL Assay | 16011455 | 1 |
| ddPPCR RCL Positive Control | 12019541 | 1 |
| ddPCR RCL Internal Control | 12019530 | 1 |
| Nuclease-Free Water | 8006877 | 1 |
| Residual DNA Quantification Supermix | 12017072 | 2 |
Configuration Options
| (Bundles or different component sets) | Catalog Number |
| QXONE Droplet Digital PCR System | 12006536 |
| QX200 AutoDG Droplet Digital PCR System consisting of: QX200 Droplet Reader Automated Droplet Generator |
1864100 1864003 1864101 |
| QX600 Droplet Digital PCR Reader | 12013328 |
| QX Manager Software, Regulatory Edition | 12012172 |
| QX Manager Software, Standard Edition | 12012171 |
| QX ONE Software, Regulatory Edition | 12012078 |
| QX Manager, Premium Edition | 12018108 |