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ZOE™ Fluorescent Cell Gallery

Images in this gallery were generated on the ZOE Cell Imager from a large selection of samples using a wide array of compatible fluorophores. Click on the images for experimental details.

  • HeLa cells were fixed in 3% PFA, permeabilized with 0.3% Triton X-100 and stained with rabbit anti-tubulin alpha 1B antibody (green), mouse anti-Lamin A/C antibody (orange/red) and counterstained with DAPI (blue).
  • HeLa cells fixed in 3% PFA, permeabilized with 0.3% Triton X-100, and stained with mouse anti-Lamin A/C antibody (green) and counterstained with DAPI (blue).
  • F-actin stained with Texas Red in BPAE cells.
  • HEK293 cells transfected with enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP).
  • Nuclei stained with Bio-Rad's PureBluTM DAPI Nuclear Staining Dye in HeLa Cells transfected with enhanced green fluorescent protein (EGFP).
  • Mitochondria stained with MitoTracker Red CMXRos, F-actin stained with Alexa Fluor 488 Phalloidin, and nuclei stained with DAPI in BPAE cells.
  • Mitochondria stained with MitoTracker Red CMXRos, F-actin stained with Alexa Fluor 488 Phalloidin, and nuclei stained with DAPI in BPAE cells.
  • Human colon adenocarcinoma on paraffin – pretreated for heat-induced epitope retrieval (HIER) using Tris/EDTA pH 9 and blocked with 10% FCS – was stained with goat anti-stem cell factor (kit-ligand) antibody and donkey anti-sheep/goat IgG:DyLight 549 secondary antibody (red) in conjunction with mouse anti-p53 antibody and rabbit anti-mouse IgG:DyLight 488 secondary antibody (green), and DAPI (blue).
  • Human colon adenocarcinoma on paraffin – pretreated for heat-induced epitope retrieval (HIER) using Tris/EDTA pH 9 and blocked with 10% FCS – was stained with goat anti-stem cell factor (kit-ligand) antibody and donkey anti-sheep/goat IgG:DyLight 549 secondary antibody (red), mouse anti-p53 antibody and rabbit anti-mouse IgG:DyLight 488 secondary antibody (green), and DAPI (blue).
  • F-actin labeled with Texas Red-X Phalloidin, microtubules labeled with mouse anti-bovine α-tubulin monoclonal antibody in conjunction with BODIPY FL Goat Anti-Mouse IgG, and nuclei labeled with DAPI in BPAE cells.
  • F-actin with Texas Red-X Phalloidin, microtubules labeled with mouse anti-bovine α-tubulin monoclonal antibody in conjunction with BODIPY FL Goat Anti-Mouse IgG, and nuclei labeled with DAPI in BPAE cells.
  • Nuclei counterstained with DAPI in a section of mouse kidney.
  • HEK293 cells from a wound-healing assay.
  • Section of mouse intestine.
  • HeLa cells.
  • HEK293 cells.
  • Onion root tip cell mitosis
  • Microtubules labeled with mouse anti-bovine α-tubulin monoclonal antibody in conjunction with BODIPY FL Goat Anti-Mouse IgG in BPAE cells.
  • F-actin stained with Alexa Fluor 488 Phalloidin in BPAE cells.
  • Fly leg.
  • Wood stem.
  • Human colon adenocarcinoma on paraffin – pretreated for heat-induced epitope retrieval (HIER) using Tris/EDTA pH 9 and blocked with 10% FCS – was stained with goat anti-stem cell factor (kit-ligand) antibody and donkey anti-sheep/goat IgG:DyLight 549 secondary antibody (red), mouse anti-p53 antibody and rabbit anti-mouse IgG:DyLight 488 secondary antibody (green), and DAPI (blue).
  • Human placenta cells on paraffin – pretreated for heat-induced epitope retrieval (HIER) using citrate pH 6 and blocked with 10% FCS – stained with goat anti-Notch 3 antibody with donkey anti-sheep/goat IgG:DyLight 549 (red) secondary antibody, mouse anti-CD34 antibody with rabbit anti-mouse IgG:DyLight 488 secondary antibody (green), and DAPI (blue).
  • Wood stem.
  • Elements of the glomeruli and convoluted tubes stained with Alexa Fluor 488 Wheat Germ Agglutinin (green fluorescent lectin), filamentous actin in the glomeruli and the brush border stained with red-orange fluorescent Alexa Fluor 568 Phalloidin, and nuclei stained with DAPI in a mouse kidney section.
  • Mucus of goblet cells stained with Alexa Fluor 350 Wheat Germ Agglutinin (blue fluorescent lectin), filamentous actin stained with red-orange fluorescent Alexa Fluor 568 Phalloidin, and nuclei stained with SYTOX Green Nucleic Acid Stain in a section of mouse intestine.
ZOE cell imager
1x105 HeLa cells were seeded on Millicell EZ Slide 4-well glass slides (sterile) and cultured in 0.5 ml RPMI medium supplemented with 2 mM CaCl2. Cells were treated with 1 μM of the apoptosis-inducing agent, staurosporine (E, F, G, H), or left untreated (A, B, C, D). pSIVA conjugated with IANBD in the presence of propidium iodide (PI) was added to the media according to kit instructions. Cells were imaged posttreatment with the ZOE Fluorescent Cell Imager at 0, 60, 120, and 270 minutes. Images AH are merged images of the red and green fluorescence channels. Early apoptotic cells were stained with pSIVA-IANBD and are shown in green, while late apoptotic/dead cells were stained with PI and are shown in red.