Western Blotting Buffers and Reagents

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Overview

Bio-Rad offers a comprehensive line of blotting membranes available in rolls or in precut sheets and essential reagents to perform colorimetric detection of proteins on western blots.
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Description

Description

Western Blotting

Bio-Rad offers an extensive line of products for detection of proteins on western blots.

Blotting Membranes for Proteins

Bio-Rad's comprehensive line of blotting membranes are available in rolls or in sheets cut to fit the Mini-PROTEAN® Tetra blotting module. Membranes are also available in preassembled blotting membrane/filter paper sandwiches.

The 0.45 µm nitrocellulose membrane is most commonly used as a transfer medium for proteins. For transfer of low molecular weight proteins (<15,000), the 0.2 µm nitrocellulose membrane provides better sensitivity, especially with immunodetection assays.

Protein Blotting Buffers and Detection Kits

Get the essential reagents to perform colorimetric detection of proteins on western blots with the convenience of premixed buffers and enzyme substrate kits. Select from a combination of binding conjugates and color detection reagents, including alkaline phosphatase (AP)- or horseradish peroxidase (HRP)-conjugated secondary antibodies and HRP-conjugated protein A or protein G.

Ordering

Ordering

items

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1610780EDU
1 L, buffer, 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, for use as western blotting wash solution; education use only
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1610734EDU
1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3; educational use only
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1610771EDU
5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3, for educational use only
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1610782EDU
1 L, blocking solution, for western blotting, 20 mM Tris, 500 mM NaCl, 1% (w/v) casein, pH 7.4; education use only
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1703932EDU
Pkg of 50, 7.5 x 10 cm, absorbent filter paper, for use with Mini-PROTEAN® Tetra Blotting Module; education use only
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1662807EDU
0.45 µm, 7 x 8.4 cm, nitrocellulose membrane (8 sheets)/filter paper (16 sheets) sandwich; education use only
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1620214EDU
Pkg of 20, 7 x 8.5 cm, precut preassembled 0.45 μm blotting membrane/filter paper sandwich; fits Ready Gel/Mini-PROTEAN gels; education use only
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1620212EDU
Pkg of 20, 7 x 8.5 cm, precut preassembled 0.2 μm blotting membrane/filter paper sandwich; fits Ready Gel/Mini-PROTEAN gels; education use only

Documents

Documents

Number Description Options
1939
Blotting Membrane Brochure, Rev G
2317
Ready-to-Run Buffers and Solutions Brochure, Rev F