RAPID'Staph Medium

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Overview

Selective medium for the direct enumeration of coagulase-positive Staphylococci in food and environmental samples. 24 hr to results.
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Description

Description

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RAPID'Staph Medium is a selective agar used for the direct enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) in products intended for human or animal consumption or in environmental samples.

Key Benefits

  • Results in 24 hr after 24 hr of enrichment
  • Highly selective and easy to read
  • Choice of two methods for easy confirmation

Principle

The principle of RAPID'Staph Medium is based on an optimized Baird-Parker formula to guarantee the detection and enumeration of S. aureus in 24 hr at 37°C. Typical staphylococci produce gray to black colonies that are shiny and convex (1–2 mm) with a clear 2–5 mm halo due to proteolysis of the egg yolk.

Presumptive colonies can be confirmed using one of the following methods:

  • Pastorex™ Staph-Plus Latex Agglutination Test (56356) with results within minutes
  • Spot inoculation on Baird-Parker + RPF agar (3563996) to confirm many colonies on the same plate

RAPID'Staph Chromogenic Assay

Enzymatic Activity
Staphylococcus
coagulase-positive

Staphylococcus
coagulase-negative
Potassium tellurite reduction + +
Egg yolk proteolysis + -

Ordering Information

RAPID'Staph Medium is available as a package of 20 ready-to-use agar plates (3563960) and as a dehydrated broth agar base (3564704).

When using the dehydrated broth base, Egg Yolk with Potassium Tellurite Supplement (3554201), (3554205) and Sulfamethazine Solution Supplement (3562682) need to be added to the molten agar prior to pouring plates.

Specifications

Specifications

Ordering

Ordering

items

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3563960
Pkg of 20, 90 mm agar plates
356-4704_160.jpg
3564704
Pkg of 1, 500 g of dehydrated agar base, enough for 8.7 L of medium
3578618
Pkg of 1, kit contains 6 x 90 ml bottles of base medium and 6 vials of rabbit plasma fibrinogen (RFP) supplement
3563996
Pkg of 20, prepared plates of Baird-Parker Agar with rabbit plasma fibrinogen (RFP), 90 mm diameter
3564814
Pkg of 1, 500 g of dehydrated agar base
3556356
Pkg of 50 tests
3556353
5 pkgs of 50 tests
355-4201_160px.jpg
3554201
Pkg of 1, 5 ml vial
355-4205_160px.jpg
3554205
Pkg of 1, 25 ml bottle
3562682
Pkg of 1, 2.5 ml vial
3578618
Pkg of 1, kit contains 6 x 90 ml bottles of base medium and 6 vials of rabbit plasma fibrinogen (RFP) supplement

Documents

Documents

Number Description Options
10000135568
RAPIDʹStaph, User Guide (English, French, German, Italian, Portuguese, Spanish)
FSD_16288
RAPID’Chromogenic Media Brochure
FSD_17933
Food Safety and Quality Reference Guide
5533
Evaluation of RAPID'Staph Medium for Enumeration of Coagulase-Positive Staphylococcus aureus in Selected Foods, Rev A
7274
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