RAPID'E.coli 2 Medium

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Overview

Medium for the direct enumeration of Escherichia coli and coliforms without confirmation. Less than 24 hr to results.
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Description

Description

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RAPID'E.coli 2 Medium is a selective chromogenic agar used for direct enumeration, without confirmation, of colonies of Escherichia coli and other coliforms in food products.

Key Benefits

  • Simultaneous enumeration of E. coli and coliforms at 37°C without further confirmation
  • Specific enumeration of E. coli at 44°C without further confirmation
  • Results in 18–24 hr
  • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Principle

The medium is based on the simultaneous detection of β-D-Glucuronidase (GLUC) and β-D-Galactosidase (GAL) activities. E. coli (GAL+/GLUC+) forms violet to pink colonies, while other coliforms (GAL+/GLUC-) form blue to green colonies.

Detection of GLUC confers a high specificity to the method because E. coli is one of the few species of Enterobacteriaceae to possess this enzyme. Growth of interfering flora is inhibited, especially at 44°C, conferring high specificity to the medium.

RAPID'E.coli 2 Chromogenic Assay

Rapid e.coli medium
Enzymatic Activity
E. coli

Other coliforms

Non-coliforms
Glucuronidase + - -
Galactosidase + + -

Two Easy Protocols

  • A single layer and a single plate per dilution for counting colonies of E. coli at 44°C
  • Counting colonies of E. coli and coliforms at 37°C within a single layer on the same plate

Ordering Information

RAPID'E.coli 2 Medium is available as a ready-to-use agar broth (3555299) or in dehydrated form (3564024).

Specifications

Specifications

Ordering

Ordering

items

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3555299
Pkg of 6, 100 ml bottles
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3555297
Pkg of 6, 200 ml bottles
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3564024
Pkg of 1, 500 g of dehydrated agar broth, enough for 13.5 L of medium
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3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
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3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions
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3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions
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3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions
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3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions
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12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions
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12013259
Pkg of 1, 500 g — for standard enrichment conditions
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12013258
Pkg of 1, 5 kg — for standard enrichment conditions

Documents

Documents

Number Description Options
10000127899
RAPID'E. coli 2, User Guide (English, French, German, Italian, Portuguese, Spanish)
FSD_16288
RAPID’Chromogenic Media Brochure
FSD_17933
Food Safety and Quality Reference Guide
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)