RAPID'E.coli 2 Medium

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RAPID'<em>E.coli </em>2 Medium

Overview



Key Benefits
  • Simultaneous enumeration of E.coli and coliforms at 37°C without further confirmation
  • Enumeration of E.coli at 44°C without further confirmation
  • Complete results in 18–24 hr
  • Gas production not necessary for differentiation of colonies

Protocol
Validation

Bio-Rad's solutions are validated by international certification bodies against standard methods, so the results you get are results you can trust.

 

AOAC

NF VALIDATION

NordVal

Health Canada
iQ-Check Real-Time PCR Methods
Campylobacter spp. Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
E.coli O157:H7 Open in new window Open in new window
Listeria monocytogenes Open in new window Open in new window Open in new window Open in new window
Listeria spp. Open in new window Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window Open in new window
Salmonella Enteritidis Open in new window
Salmonella Typhimurium Open in new window
STEC 7 major O-groups Open in new window
STEC virulence genes Open in new window
Vibrio spp. Open in new window
RAPID'Chromogenic Methods
Bacillus cereus Open in new window
Campylobacter spp. Open in new window
Coliforms Open in new window Open in new window Open in new window Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
Escherichia coli Open in new window Open in new window Open in new window Open in new window
E. coli O157:H7 Open in new window Open in new window
Listeria monocytogenes

Detection
Enumeration
AL


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Listeria spp. Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window
Staphylococcus aureus Open in new window

* Alternative analytical methods for agrifood — http://nf-validation.afnor.org/en

Medium for the direct enumeration of Escherichia coli and coliforms without confirmation. Less than 24 hr to results.
Read full description

Ordering Information


Description

Talk with a Specialist

RAPID'E.coli 2 Medium is a selective chromogenic agar used for direct enumeration, without confirmation, of colonies of Escherichia coli and other coliforms in food products.

Key Benefits

  • Simultaneous enumeration of E. coli and coliforms at 37°C without further confirmation
  • Specific enumeration of E. coli at 44°C without further confirmation
  • Results in 18–24 hr
  • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Principle

The medium is based on the simultaneous detection of β-D-Glucuronidase (GLUC) and β-D-Galactosidase (GAL) activities. E. coli (GAL+/GLUC+) forms violet to pink colonies, while other coliforms (GAL+/GLUC-) form blue to green colonies.

Detection of GLUC confers a high specificity to the method because E. coli is one of the few species of Enterobacteriaceae to possess this enzyme. Growth of interfering flora is inhibited, especially at 44°C, conferring high specificity to the medium.

RAPID'E.coli 2 Chromogenic Assay

Rapid e.coli medium
Enzymatic Activity
E. coli

Other coliforms

Non-coliforms
Glucuronidase + - -
Galactosidase + + -

Two Easy Protocols

  • A single layer and a single plate per dilution for counting colonies of E. coli at 44°C
  • Counting colonies of E. coli and coliforms at 37°C within a single layer on the same plate

Ordering Information

RAPID'E.coli 2 Medium is available as a ready-to-use agar broth (3555299) or in dehydrated form (3564024).


Specifications

Assay type Chromogenic media
Organism E. coli, coliforms

Ordering

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RAPID'E.coli 2 Agar, ready to use

3555299
Pkg of 6, 100 ml bottles

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$43.00 
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RAPID'E.coli 2 Agar, ready to use

3555297
Pkg of 6, 200 ml bottles

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$96.00 
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RAPID'E.coli 2 Agar, dehydrated

3564024
Pkg of 1, 500 g of dehydrated agar broth, enough for 13.5 L of medium

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$454.00 
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Buffered Peptone Water Plus

3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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$28.00 
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Buffered Peptone Water Plus

3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions

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$113.00 
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Buffered Peptone Water Plus

3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions

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$114.00 
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Buffered Peptone Water Plus

3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

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$78.00 
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Buffered Peptone Water Plus

3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions

List Price: 
 
$716.00 
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Buffered Peptone Water Standard

12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions

List Price: 
 
$99.00 
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Buffered Peptone Water Standard, dehydrated

12013259
Pkg of 1, 500 g — for standard enrichment conditions

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$68.00 
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Buffered Peptone Water Standard, dehydrated

12013258
Pkg of 1, 5 kg — for standard enrichment conditions

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$622.00 
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Documents

Number Description Options
10000127899 RAPID'E. coli 2, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
FSD_16288 RAPID’Chromogenic Media Brochure Click to download
FSD_17933 Food Safety and Quality Reference Guide Click to download
7274 Complete Solutions for Service Laboratories Click to download
10000128740 Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
10000128738 Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download

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