Profinity IMAC Resins

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Overview

Profinity IMAC resin is an affinity chromatography support for the purification of recombinant histidine (His)-tagged proteins.
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Description

Description

Profinity IMAC resin is an affinity chromatography support for the purification of recombinant histidine (His)-tagged proteins. Immobilized metal affinity chromatography (IMAC) is a powerful purification technique that relies on the affinity that His has for immobilized transition metals. Profinity IMAC resin, based on UNOsphere beads, contains iminodiacetic acid (IDA) as the chelating ligand for di- or trivalent metal ions. Its chemical structure allows highly selective binding of recombinant His-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.

Profinity IMAC resin offers high capacity at high flow rates. It can be used under either nondenaturing or denaturing conditions and is suitable for purification using liquid chromatographic instrumentation, gravity-flow columns, or spin columns.

The resin is available in two forms: uncharged and precharged with Ni2+. The uncharged form can be charged with the metal ion of your choice for even greater purification flexibility.

Features of Profinity IMAC resins include:

  • Optimal ligand density for higher purity of target protein
  • Superb mechanical strength
  • Excellent pressure-flow properties for high maximum operating pressures and high flow rates, which allow rapid purification as well as rapid column cleaning and re-equilibration
  • Stable from pH 1 to 14
  • Compatible with denaturing agents, detergents, and reducing agents

For more information, request bulletin 5456.

Larger volumes and special packaging for industrial applications are available upon request.

Ordering

Ordering

items

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1560121
Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography
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1560123
Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography
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1560125
Pkg of 1, 500 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography
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1560127
Pkg of 1, 1 L, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography
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1560131
Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography
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1560133
Pkg of 1, 25 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography
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1560135
Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography
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1560137
Pkg of 1, 500 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

Kits & Reagents

Kits & Reagents

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6200235
Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia IMAC buffer kits (#620-0221)
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6200225
Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges
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7324614
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324612
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324610
Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324651
Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue

Documents

Documents

Number Description Options
5456
Profinity™ IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification, Rev B
3193
Profinity IMAC Resin Product Information Sheet
5283
Profinity IMAC Ni-Charged Resin Flier, Rev A
5251
Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins, Rev A
5501
Rapid, Efficient Purification and Evaluation of His-Tagged Proteins, Rev A
5444
Bio-Scale Mini Profinity IMAC Cartridge Product Information Sheet, Rev A
5514
Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A
10001677
Instruction Manual, Profinity IMAC Resins, Rev B
10005455
Instruction Manual, Bio-Scale™ Mini Profinity™ IMAC Cartridges, 1 and 5 ml, Rev D
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A