Profinity IMAC Resins

Overview

Affinity Chromatography Resin Selection Guide

	Matrix	Functional Group	Specificity	Capacity	Working pH	Pressure Limit	Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC	High capacity, pressure-stable polymer based on UNOsphere™ beads	NTA charged with Ni²⁺	Histidine	≥40 mg/ml*	2–14	45 psi (3.1 bar)	Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC	Pressure-stable polymer based on UNOsphere beads	IDA, available charged with Ni²⁺ and uncharged	Histidine	≥15 mg/ml*	1–14	100 psi (6.8 bar)	Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST**	Pressure-stable polymer based on UNOsphere beads	Immobilized glutathione	GST-tagged proteins	≥10 mg/ml	1–14	45 psi (3.1 bar)	Purification of recombinant GST-tagged proteins
Profinity eXact™***	Crosslinked 6% agarose	Subtilisin protease	Subtilisin prodomain	≥3 mg/ml tag-free protein	2–10	10 psi (minus system pressure)	Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel^® Protein A	Crosslinked agarose	Protein A 2 mg/ml	IgG		2–10	15 psi (1 bar)	Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep^® Protein A	Pressure-stable polymer	Protein A 2 mg/ml	IgG		2–10	1,000 psi (70 bar)	Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue	Crosslinked agarose	Cibacron Blue F3GA 1.9 mg/ml	Albumin; general	≥11 mg/ml	2–10	15 psi (1 bar)	Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue	Crosslinked agarose	Cibacron Blue F3GA and DEAE	Albumin and serum proteins	0.14 ml serum/ml gel	2–10	15 psi (1 bar)	Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue	Crosslinked agarose	Cibacron Blue F3GA and CM	Albumin and serum proteins	0.17–0.5 ml serum/ml gel	2–11	15 psi (1 bar)	Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin	Pressure-stable polymer	Polymyxin 2–4 mg/ml	Endotoxins	>5 mg/ml	2–10	1,000 psi (70 bar)	Endotoxin removal
Affi-Gel Boronate	Polyacrylamide gel	Boronate 1.05 ± 0.15	cis-diols meq/g	130 µmol sorbitol/ml	2–10	15 psi (1 bar)	Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide	Pressure-stable polymer based on UNOsphere beads	Epoxy group	Nucleophiles; amini, thiol, -COOH	36–40 mg/ml lgG	1–14	Up to 80 psi (5.5 bar)	Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10	Crosslinked agarose	N-hydroxy-succinimide ≥10 µmol/ml	-NH₂	35 mg/ml	3–11	15 psi (1 bar)	For coupling proteins with pI 6.5–11
Affi-Gel 15	Crosslinked agarose	N-hydroxy-succinimide ≥9 µmol/ml	-NH₂	35 mg/ml	3–11	15 psi (1 bar)	For coupling proteins with pI 6.5–11
Affi-Gel Hz	Crosslinked agarose	Hydrazide	Oxidized carbohydrates	1–5 mg/ml	2–10	15 psi (1 bar)	Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102	Crosslinked agarose	-NH₂ 16 ± 4 meq/ml	-COOH	40 mg	2–11	15 psi (1 bar)	Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

Chemical Compatibilities

Chemical Compatibilities for Profinity IMAC Resins

Chemical compatibilities for binding of target protein to Profinity IMAC Ni-charged resin*

Reagent	Concentration**
Buffer Reagents
Tris	50 mM
HEPES	50 mM
MOPS	50 mM
Sodium or potassium phosphate	50 mM
Chelating Agents
EDTA, EGTA	0.1 mM
Sulfhydryl Reagents
β-Mercaptoethanol	30 mM
DTT	5 mM
TCEP	10 mM
Detergents
Nonionic detergents (Triton, Tween, NP-40)	5%
Cationic detergents (CTAB)	1% (care must be taken to avoid protein precipitation)
Zwitterionic detergents (CHAPS, CHAPSO)	5%
Anionic detergents (SDS, Sarkosyl)	1%
Denaturing Agents
Guanidine HCl	6-M
Urea	8-M
Other Additives
NaCl	2 M (at least 300 mM NaCl should be included in buffers)
MgCl₂	100 mM (HEPES or Tris should be used to prevent precipitation)
CaCl₂	10 mM (HEPES or Tris should be used to prevent precipitation)
Glycerol	20%
Ethanol	20%
Imidazole	5–10 mM in wash buffer 500 mM for elution
Citrate	80 mM

* In order to determine the efficacy of protein binding and recovery, tests were performed using a purified 75 kD protein in a static mode. All tests were carried out using Micro Bio-Spin columns packed with Profinity IMAC resin and eluted by centrifugation.
** Profinity IMAC binding capacities are unaffected with typical reagents used for histidine-tagged protein purification, up to the concentrations given.

Maximum Operating Pressures

Comparison of Maximum Operating Pressures

Comparison of maximum operating pressure for different IMAC resins. The pressure values shown are the estimated maximum operating pressures for each resin. Beyond these pressures, the column beds are seriously compressed and damaged. Resins were converted to 50% (v/v) slurry in 20 mM sodium phosphate buffer and packed in a 1.1 x 20 cm column to a bed height of 20 cm up to 42 psi (3 bar). Flow rates were then increased stepwise by 200 cm/hr and held for 2 min at each step. The pressure-flow curve for Profinity IMAC resin became nonlinear only at pressures above 109 psi (7.5 bar), the point defined as the intersection of the two tangents on the pressure-flow curve.

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Profinity IMAC resin is an affinity chromatography support for the purification of recombinant histidine (His)-tagged proteins. Immobilized metal affinity chromatography (IMAC) is a powerful purification technique that relies on the affinity that His has for immobilized transition metals. Profinity IMAC resin, based on UNOsphere beads, contains iminodiacetic acid (IDA) as the chelating ligand for di- or trivalent metal ions. Its chemical structure allows highly selective binding of recombinant His-tagged proteins when charged with Ni²⁺ or other transition metals, such as Zn²⁺ or Cu²⁺.

Profinity IMAC resin offers high capacity at high flow rates. It can be used under either nondenaturing or denaturing conditions and is suitable for purification using liquid chromatographic instrumentation, gravity-flow columns, or spin columns.

The resin is available in two forms: uncharged and precharged with Ni²⁺. The uncharged form can be charged with the metal ion of your choice for even greater purification flexibility.

Features of Profinity IMAC resins include:

Optimal ligand density for higher purity of target protein
Superb mechanical strength
Excellent pressure-flow properties for high maximum operating pressures and high flow rates, which allow rapid purification as well as rapid column cleaning and re-equilibration
Stable from pH 1 to 14
Compatible with denaturing agents, detergents, and reducing agents

For more information, request bulletin 5456.

Larger volumes and special packaging for industrial applications are available upon request.

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Profinity™ IMAC Resin, uncharged, 10 ml

1560121
Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

2 1560121 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0121_0921120658495.xml 1560121 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0teo5 2017-11-14 07:08:02 true true internet/product_SKU jao0teo5 templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0121_0921120658495.xml LSR 2019-05-04 18:20:15 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556917348251 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:20:15 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0121_0921120658495.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0121_0921120658495.xml 1560121-profinity-imac-resin-uncharged-10-ml 1560121 Profinity IMAC Uncharged Resin Profinity™ IMAC Resin, uncharged, 10 ml Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography 10 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0121_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0121_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0121_feature.jpg preview ,VN RepEx 156-0121 10 ml, ready-to-use support for immobilized metal affinity chromatography 1560121, profinity, imac, uncharged, resin, immobilized, metal, affinity, chromatography, purification, ready-to-use, support, media, ml 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1–14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top">1560121</td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href="/en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale™ Mini Profinity™ IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale™ Mini Nuvia™ IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>®</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC™ Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic™ Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia™ Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-uncharged-10ml-1560121.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-uncharged-10ml-1560121.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, uncharged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false $90.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560121&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560121&CartQuote=QT&quantity= 1332 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560121&AddToDefaultWishList=true&quantity= 13111000 90.00 1560121

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Profinity™ IMAC Resin, uncharged, 50 ml

1560123
Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

3 1560123 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0123_0921120658518.xml 1560123 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0temv 2017-11-14 07:08:02 true true internet/product_SKU jao0temv templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0123_0921120658518.xml LSR 2019-05-04 18:20:27 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556917348304 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:20:27 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0123_0921120658518.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0123_0921120658518.xml 1560123-profinity-imac-resin-uncharged-50-ml 1560123 Profinity IMAC Uncharged Resin Profinity™ IMAC Resin, uncharged, 50 ml Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography 50 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0123_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0123_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0123_feature.jpg preview ,VN RepEx 156-0123 50 ml, ready-to-use support for immobilized metal affinity chromatography 1560123, profinity, imac, uncharged, resin, immobilized, metal, affinity, chromatography, purification, ready-to-use, support, media, 50, ml 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1–14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top">1560123</td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href="/en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale™ Mini Profinity™ IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale™ Mini Nuvia™ IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>®</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC™ Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic™ Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia™ Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-uncharged-50ml-1560123.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-uncharged-50ml-1560123.html Ligand 1775 1 0 Form 1659 50% slurry in 20% ethanol, uncharged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false $389.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560123&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560123&CartQuote=QT&quantity= 1332 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560123&AddToDefaultWishList=true&quantity= 13111000 389.00 1560123

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(Discontinued) Profinity™ IMAC Resin, uncharged, 500 ml

1560125
Pkg of 1, 500 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

Profinity™ IMAC Resin, uncharged, 10 ml (1560121)
Profinity™ IMAC Resin, uncharged, 50 ml (1560123)

Profinity™ IMAC Resin, uncharged, 1 L

1560127
Pkg of 1, 1 L, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

5 1560127 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0127_0921120658537.xml 1560127 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0teni 2017-11-14 07:08:02 true true internet/product_SKU jao0teni templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0127_0921120658537.xml LSR 2019-05-04 18:20:21 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556917348463 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:20:21 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0127_0921120658537.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0127_0921120658537.xml 1560127-profinity-imac-resin-uncharged-1-l 1560127 Profinity IMAC Uncharged Resin Profinity™ IMAC Resin, uncharged, 1 L Pkg of 1, 1 L, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography 1 L, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 1 L, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0127_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0127_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0127_feature.jpg preview ,VN RepEx 156-0127 1 L, ready-to-use support for immobilized metal affinity chromatography 1560127, profinity, imac, uncharged, resin, immobilized, metal, affinity, chromatography, purification, ready-to-use, support, media, 1L 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Use with batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1–14)</li> <li>Use under native or denaturing conditions</li> <li>Single-step purification from crude samples, or use for contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top">1560127</td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale™ Mini Profinity™ IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale™ Mini Nuvia™ IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>®</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC™ Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic™ Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia™ Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-uncharged-1l-1560127.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-uncharged-1l-1560127.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, uncharged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false null false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560127&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560127&CartQuote=QT&quantity= 1332 false true false 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Profinity™ IMAC Resin, Ni-charged, 10 ml

1560131
Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

6 1560131 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0131_0921120658411.xml 1560131 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0temg 2017-11-14 07:08:02 true true internet/product_SKU jao0temg templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0131_0921120658411.xml LSR 2019-05-04 18:20:26 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556917348534 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:20:26 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0131_0921120658411.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0131_0921120658411.xml 1560131-profinity-imac-resin-ni-charged-10-ml 1560131 Profinity IMAC Ni-Charged Resin Profinity™ IMAC Resin, Ni-charged, 10 ml Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography 10 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0131_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0131_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0131_feature.jpg preview ,VN RepEx 156-0131 10 ml, ready-to-use support for immobilized metal affinity chromatography 1560131, profinity, imac, nickel-charged, ni-charged, ni, nickel, charged, resin, immobilized, metal, affinity, chromatography, ready-to-use, support, media, hisitidine, his, tagged, his-tagged, protein, puritifcation, ml 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1–14)</li> <li> Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top">1560131</td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href="/en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale™ Mini Profinity™ IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale™ Mini Nuvia™ IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>®</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC™ Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic™ Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia™ Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-ni-charged-10ml-1560131.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-ni-charged-10ml-1560131.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, Ni-charged or uncharged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false $113.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560131&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560131&CartQuote=QT&quantity= 1332 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560131&AddToDefaultWishList=true&quantity= 13111000 113.00 1560131

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Profinity™ IMAC Resin, Ni-charged, 25 ml

1560133
Pkg of 1, 25 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

7 1560133 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0133_0921120658478.xml 1560133 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0ten4 2017-11-14 07:08:02 true true internet/product_SKU jao0ten4 templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0133_0921120658478.xml LSR 2019-05-04 18:20:12 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556917348581 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:20:12 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0133_0921120658478.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0133_0921120658478.xml 1560133-profinity-imac-resin-ni-charged-25-ml 1560133 Profinity IMAC Ni-Charged Resin Profinity™ IMAC Resin, Ni-charged, 25 ml Pkg of 1, 25 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography 25 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 25 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0133_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0133_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0133_feature.jpg preview ,VN RepEx 156-0133 25 ml, ready-to-use support for immobilized metal affinity chromatography 1560133, profinity, imac, nickel-charged, ni-charged, ni, nickel, charged, resin, immobilized, metal, affinity, chromatography, ready-to-use, support, media, hisitidine, his, tagged, his-tagged, protein, puritifcation, 25, ml 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1–14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top">1560133</td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href="/en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale™ Mini Profinity™ IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale™ Mini Nuvia™ IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>®</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC™ Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic™ Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia™ Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-ni-charged-25ml-1560133.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-ni-charged-25ml-1560133.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, Ni-charged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false $276.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560133&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560133&CartQuote=QT&quantity= 1332 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560133&AddToDefaultWishList=true&quantity= 13111000 276.00 1560133

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Profinity™ IMAC Resin, Ni-charged, 100 ml

1560135
Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

8 1560135 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0135_0921120658449.xml 1560135 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0teoq 2017-11-14 07:08:02 true true internet/product_SKU jao0teoq templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0135_0921120658449.xml LSR 2019-05-04 18:20:33 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998737977 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:20:33 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0135_0921120658449.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0135_0921120658449.xml 1560135-profinity-imac-resin-ni-charged-100-ml 1560135 Profinity IMAC Ni-Charged Resin Profinity™ IMAC Resin, Ni-charged, 100 ml Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography 100 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography /webroot/web/images/ps/products/affinity_media/sku_thumb/global/156-0135_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/156-0135_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/156-0135_feature.jpg preview ,VN RepEx 156-0135 100 ml, ready-to-use support for immobilized metal affinity chromatography 1560135, profinity, imac, nickel-charged, ni-charged, ni, nickel, charged, resin, immobilized, metal, affinity, chromatography, ready-to-use, support, media, hisitidine, his, tagged, his-tagged, protein, puritifcation, 100, ml 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1–14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top">1560135</td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href="/en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale™ Mini Profinity™ IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale™ Mini Nuvia™ IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>®</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC™ Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic™ Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia™ Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-ni-charged-100ml-1560135.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-ni-charged-100ml-1560135.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, Ni-charged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false $1,011.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560135&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560135&CartQuote=QT&quantity= 1332 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560135&AddToDefaultWishList=true&quantity= 13111000 1011.00 1560135

List Price:		$1,011.00 Loading
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Profinity™ IMAC Resin, Ni-charged, 500 ml

1560137
Pkg of 1, 500 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

9 1560137 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0137_0921120658390.xml 1560137 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0temt 2017-11-14 07:08:02 true true internet/product_SKU jao0temt templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0137_0921120658390.xml LSR 2019-05-04 18:20:10 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998738020 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:20:10 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0137_0921120658390.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0137_0921120658390.xml 1560137-profinity-imac-resin-ni-charged-500-ml 1560137 Profinity IMAC Ni-Charged Resin Profinity™ IMAC Resin, Ni-charged, 500 ml Pkg of 1, 500 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography 500 ml, ready-to-use support for immobilized metal affinity chromatography Pkg of 1, 500 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography /webroot/web/images/ps/products/affinity_media/sku_thumb/global/156-0137_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/156-0137_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/156-0137_feature.jpg preview ,VN RepEx 156-0137 500 ml, ready-to-use support for immobilized metal affinity chromatography 1560137, profinity, imac, nickel-charged, ni-charged, ni, nickel, charged, resin, immobilized, metal, affinity, chromatography, ready-to-use, support, media, hisitidine, his, tagged, his-tagged, protein, puritifcation, 500, ml 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1–14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top">1560137</td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml?ID=1560121">1560121 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href="/en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale™ Mini Profinity™ IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale™ Mini Nuvia™ IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>®</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC™ Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic™ Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia™ Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-ni-charged-500ml-1560137.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-ni-charged-500ml-1560137.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, Ni-charged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false $4,559.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560137&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560137&CartQuote=QT&quantity= 1332 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560137&AddToDefaultWishList=true&quantity= 13111000 4559.00 1560137

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Profinia IMAC Buffer Kit

6200221
Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system

10 6200221 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0221_0921120618995.xml 6200221 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0tenr 2017-11-14 07:08:04 true true internet/product_SKU jao0tenr templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0221_0921120618995.xml LSR 2019-05-04 20:55:44 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998741778 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:44 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0221_0921120618995.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0221_0921120618995.xml 6200221-profinia-imac-buffer-kit 6200221 Profinia Native IMAC Buffer Kit Profinia IMAC Buffer Kit Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system /webroot/web/images/lsr/products/chromatography/sku_thumb/global/620-0221_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/620-0221_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/620-0221_feature.jpg ,VN RepEx 620-0221 Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false $382.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=6200221&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=6200221&CartQuote=QT&quantity= 1278 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=6200221&AddToDefaultWishList=true&quantity= 12161500 382.00 6200221

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Profinia IMAC Purification Kit

6200235
Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia IMAC buffer kits (#620-0221)

13 6200235 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0235_0921120619439.xml 6200235 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0temi 2017-11-14 07:08:04 true true internet/product_SKU jao0temi templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0235_0921120619439.xml LSR 2019-05-04 20:55:41 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998742240 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:41 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0235_0921120619439.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0235_0921120619439.xml 6200235-profinia-imac-purification-kit 6200235 Profinia Native IMAC Purification Kit Profinia IMAC Purification Kit Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia IMAC buffer kits (#620-0221) Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia native IMAC buffer kits (#620-0221) Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia IMAC buffer kits (#620-0221) /webroot/web/images/lsr/products/chromatography/sku_thumb/global/620-0235_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/620-0235_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/620-0235_feature.jpg ,VN RepEx 620-0235 Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia native IMAC buffer kits (#620-0221) 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false $965.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=6200235&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=6200235&CartQuote=QT&quantity= 1278 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=6200235&AddToDefaultWishList=true&quantity= 41115700 965.00 6200235

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Profinia IMAC Purification Kit

6200225
Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges

14 6200225 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0225_0921120618815.xml 6200225 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0teml 2017-11-14 07:08:04 true true internet/product_SKU jao0teml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0225_0921120618815.xml LSR 2019-05-04 20:55:55 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998741945 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:55 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0225_0921120618815.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/620-0225_0921120618815.xml 6200225-profinia-imac-purification-kit 6200225 Profinia Native IMAC Purification Kit Profinia IMAC Purification Kit Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges Kit for use with the Profinia protein purification system, includes Profinia native IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges /webroot/web/images/lsr/products/chromatography/sku_thumb/global/620-0225_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/620-0225_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/620-0225_feature.jpg ,VN RepEx 620-0225 Kit for use with the Profinia protein purification system, includes Profinia native IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false $562.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=6200225&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=6200225&CartQuote=QT&quantity= 1278 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=6200225&AddToDefaultWishList=true&quantity= 12161500 562.00 6200225

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Bio-Scale Mini Profinity IMAC Cartridges

7324614
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

15 7324614 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4614_0921120618916.xml 7324614 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0teol 2017-11-14 07:08:06 true true internet/product_SKU jao0teol templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4614_0921120618916.xml LSR 2019-05-04 20:55:47 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998747229 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:47 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4614_0921120618916.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4614_0921120618916.xml 7324614-bio-scale-mini-profinity-imac-cartridges 7324614 Bio-Scale Mini Profinity IMAC Cartridges Bio-Scale Mini Profinity IMAC Cartridges Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4614_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4614_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/732-4614_feature.jpg RepEx 732-4614 Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false $606.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324614&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324614&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324614&AddToDefaultWishList=true&quantity= 41115700 606.00 7324614

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Bio-Scale Mini Profinity IMAC Cartridge

7324612
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

16 7324612 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4612_0921120619067.xml 7324612 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0teo6 2017-11-14 07:08:06 true true internet/product_SKU jao0teo6 templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4612_0921120619067.xml LSR 2019-05-04 20:55:59 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998747187 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:59 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4612_0921120619067.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4612_0921120619067.xml 7324612-bio-scale-mini-profinity-imac-cartridge 7324612 Bio-Scale Mini Profinity IMAC Cartridge Bio-Scale Mini Profinity IMAC Cartridge Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4612_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4612_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/732-4612_feature.jpg RepEx 732-4612 Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false $173.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324612&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324612&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324612&AddToDefaultWishList=true&quantity= 41115700 173.00 7324612

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Bio-Scale Mini Profinity IMAC Cartridges

7324610
Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

17 7324610 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4610_0921120619018.xml 7324610 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0teoa 2017-11-14 07:08:06 true true internet/product_SKU jao0teoa templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4610_0921120619018.xml LSR 2019-05-04 20:56:07 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998747145 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:07 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4610_0921120619018.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4610_0921120619018.xml 7324610-bio-scale-mini-profinity-imac-cartridges 7324610 Bio-Scale Mini Profinity IMAC Cartridges Bio-Scale Mini Profinity IMAC Cartridges Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4610_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4610_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/732-4610_feature.jpg RepEx 732-4610 Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 7324610, bio-scale, mini, profinity, imac, cartridges, prepacked, nickel, ni-charged, column, 40 mmx5.6 mm, biologic, chromatography, affinity, media, resin, protein, separation, purification false $254.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324610&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324610&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324610&AddToDefaultWishList=true&quantity= 41115700 254.00 7324610

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Bio-Scale Mini Affinity Sampler Pack

7324651
Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue

18 7324651 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4651_0921120612199.xml 7324651 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0tenn 2017-11-14 07:08:06 true true internet/product_SKU jao0tenn templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4651_0921120612199.xml LSR 2019-05-04 20:56:04 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998747568 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:04 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4651_0921120612199.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4651_0921120612199.xml 7324651-bio-scale-mini-affinity-sampler-pack 7324651 Bio-Scale Mini Affinity Sampler Pack Bio-Scale Mini Affinity Sampler Pack Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue /webroot/web/images/ps/products/affinity_media/sku_thumb/global/732-4651_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/732-4651_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/732-4651_feature.jpg RepEx 732-4651 Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false $735.00 false $ null false false true true false USD true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324651&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324651&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324651&AddToDefaultWishList=true&quantity= 41116100 735.00 7324651

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Number	Description	Options
5456	Profinity™ IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification, Rev B	Click to download
3193	Profinity IMAC Resin Product Information Sheet	Click to download
5283	Profinity IMAC Ni-Charged Resin Flier, Rev A	Click to download
5251	Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins, Rev A	Click to download
5501	Rapid, Efficient Purification and Evaluation of His-Tagged Proteins, Rev A	Click to download
5444	Bio-Scale Mini Profinity IMAC Cartridge Product Information Sheet, Rev A	Click to download
5514	Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A	Click to download
10001677	Instruction Manual, Profinity IMAC Resins, Rev B	Click to download
10005455	Instruction Manual, Bio-Scale™ Mini Profinity™ IMAC Cartridges, 1 and 5 ml, Rev D	Click to download
6810	Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A	Click to download

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Profinity IMAC Resins

Overview

Profinity™ IMAC Resin, uncharged, 10 ml

Profinity™ IMAC Resin, uncharged, 50 ml

(Discontinued) Profinity™ IMAC Resin, uncharged, 500 ml

Profinity™ IMAC Resin, uncharged, 1 L

Profinity™ IMAC Resin, Ni-charged, 10 ml

Profinity™ IMAC Resin, Ni-charged, 25 ml

Profinity™ IMAC Resin, Ni-charged, 100 ml

Profinity™ IMAC Resin, Ni-charged, 500 ml

Profinia IMAC Buffer Kit

Profinia IMAC Purification Kit

Profinia IMAC Purification Kit

Bio-Scale Mini Profinity IMAC Cartridges

Bio-Scale Mini Profinity IMAC Cartridge

Bio-Scale Mini Profinity IMAC Cartridges

Bio-Scale Mini Affinity Sampler Pack

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Profinity IMAC Resins

Overview

I cannot find the Certificate of Analysis I am looking for. Help!

Where can I find the catalog number, SKU number, or product number?

Where can I find the lot or control number?

Why are Certificates of Analysis not on the Documents tab?

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

Can I get a Certificate of Analysis even if my item is expired?

Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?

Why does it say "Not Available" for a Certificate of Analysis listed in the search results?

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Documents

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