Profinity IMAC Resins

Profinity IMAC Resins

Profinity IMAC resin is an affinity chromatography support for the purification of recombinant histidine (His)-tagged proteins.

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Overview

Profinity IMAC resin is an affinity chromatography support for the purification of recombinant histidine (His)-tagged proteins. Immobilized metal affinity chromatography (IMAC) is a powerful purification technique that relies on the affinity that His has for immobilized transition metals. Profinity IMAC resin, based on UNOsphere beads, contains iminodiacetic acid (IDA) as the chelating ligand for di- or trivalent metal ions. Its chemical structure allows highly selective binding of recombinant His-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.

Profinity IMAC resin offers high capacity at high flow rates. It can be used under either nondenaturing or denaturing conditions and is suitable for purification using liquid chromatographic instrumentation, gravity-flow columns, or spin columns.

The resin is available in two forms: uncharged and precharged with Ni2+. The uncharged form can be charged with the metal ion of your choice for even greater purification flexibility.

Features of Profinity IMAC resins include:

  • Optimal ligand density for higher purity of target protein
  • Superb mechanical strength
  • Excellent pressure-flow properties for high maximum operating pressures and high flow rates, which allow rapid purification as well as rapid column cleaning and re-equilibration
  • Stable from pH 1 to 14
  • Compatible with denaturing agents, detergents, and reducing agents

For more information, request bulletin 5456.

Larger volumes and special packaging for industrial applications are available upon request.

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1560121

Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

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1560123

Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

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1560127

Pkg of 1, 1 L, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

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1560131

Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

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1560133

Pkg of 1, 25 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

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1560135

Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

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1560137

Pkg of 1, 500 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

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6200221
Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system
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12009298

Pkg of 5, 1 ml, prepacked Ni-charged column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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12009299

Pkg of 1, 5 ml, prepacked Ni-charged column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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12009300

Pkg of 5, 5 ml, prepacked Ni-charged column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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