Profinity IMAC Resin

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Overview

Profinity IMAC (immobilized metal affinity chromatography) Resin is an affinity chromatography support for protein purification of recombinant histidine-tagged products.
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Description

Description

Profinity IMAC resin is an affinity chromatography support for the purification of recombinant histidine (His)-tagged proteins. Immobilized metal affinity chromatography (IMAC) is a powerful purification technique that relies on the affinity that His has for immobilized transition metals. Profinity IMAC resin, based on UNOsphere beads, contains iminodiacetic acid (IDA) as the chelating ligand for di- or trivalent metal ions. Its chemical structure allows highly selective binding of recombinant His-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.

Profinity IMAC offers high capacity at high flow rates. It can be used under either nondenaturing or denaturing conditions, and is suitable for purification using liquid chromatographic instrumentation, gravity-flow columns, or spin columns.

The resin is available in two forms: uncharged and precharged with Ni2+. The uncharged form can be charged with the metal ion of your choice for even greater purification flexibility.

Features of Profinity IMAC resins include:

  • Optimal ligand density for higher purity of target protein
  • Superb mechanical strength
  • Excellent pressure-flow properties for high maximum operating pressures and high flow rates, which allow rapid purification as well as rapid column cleaning and re-equilibration
  • Stable from pH 1 to 14
  • Compatible with denaturing agents, detergents, and reducing agents

Ordering

Ordering

items

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1560131
Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography
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1560133
Pkg of 1, 25 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography
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1560135
Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography
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1560137
Pkg of 1, 500 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography
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1560121
Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography
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1560123
Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography
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1560125
Pkg of 1, 500 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography
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1560127
Pkg of 1, 1 L, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography
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12009298
Pkg of 5, 1 ml, prepacked Ni-charged column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system
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12009299
Pkg of 1, 5 ml, prepacked Ni-charged column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system
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12009300
Pkg of 5, 5 ml, prepacked Ni-charged column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

Documents

Documents

Number Description Options
5456
Profinity™ IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification, Rev B
10001677
Instruction Manual, Profinity IMAC Resins, Rev B
3193
Profinity IMAC Resin Product Information Sheet
5283
Profinity IMAC Ni-Charged Resin Flier, Rev A