Profinity Exact Purification Resin

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Overview

Profinity eXact Affinity Purification Resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix.
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Description

Description

Profinity eXact Purification Resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. The resulting purity of the eluted protein is typically higher than that for other affinity-tag systems due to the specific recognition (KD < 100 pM) that subtilisin has for its prodomain sequence (the Profinity eXact Tag). Once the Profinity eXact fusion protein has been captured by the Profinity eXact Purification Resin — and the cleavage reaction has been triggered with a sodium fluoride-containing buffer — the affinity tag is precisely cleaved at the C-terminus of the cleavage recognition sequence (EEDKLFKAL). The result is a quickly purified protein product that lacks any amino acid residues derived from the Profinity eXact Tag.

Features and Benefits

  • Purification and processing of fusion-tagged proteins in a single-step
  • On-column cleavage in as little as 30 min
  • No protease addition required
  • Precise cleavage at N-terminus to generate native protein sequence

The following convenient packaging configurations allow easy evaluation of Profinity eXact technology:

  • Profinity eXact Resin — 10 ml affinity resin supplied in 100 mM sodium phosphate, pH 7.2 containing 0.02% sodium azide
  • Profinity eXact Mini Spin Columns — 10-pack of single-use columns that contain 0.1 ml settled resin in addition to all required collection tubes for binding, washing, and elution steps. Fit all benchtop centrifuges
  • Profinity eXact Mini Spin Starter Kit — comprehensive 10-pack kit that ensures purification success upon initial trial. Includes 10 prepacked spin columns, collection tubes, lysis reagent, buffers for purification, and lyophilized MBP control protein lysate
  • Bio-Scale Mini Profinity eXact Cartridges — 1 ml and 5 ml cartridges that can be used with BioLogic chromatography systems, the Profinia Protein Purification System, a peristaltic pump, or with appropriate column adaptors with any other chromatography system
  • Profinity eXact Expression and Purification Starter Kit — includes two kits:
    • 1 Profinity eXact Cloning and Expression Starter Kit for the cloning and expression of a target gene using pPAL vectors and competent cells
    • 1 Profinity eXact Mini Spin Purification Starter Kit for single-step purification and on-column cleavage of the tagged protein

More Information

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1563005
10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide
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1563008
Kit includes 1 Profinity eXact cloning and expression starter kit (20 reactions) and 1 Profinity eXact mini spin purification starter kit (10 spin columns)
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1563006
Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers

Kits & Reagents

Kits & Reagents

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1563001
Includes 25 μl of 20 ng/μl pPAL7 restriction-independent cloning (RIC)-ready expression vector; sufficient for 20 reactions
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1708237
Colorimetric detection (µg) kit, includes secondary antibody, optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots)
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1708235
Colorimetric detection (µg) kit, includes optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots)
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1563004
Pkg of 1 vial, mouse monoclonal antibody, anti subtilisin protease prodomain antibody, recognizes Profinity eXact-tagged fusion proteins
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1563000
Includes 25 µl of 20 ng/µl pPAL RIC-ready and 100 µl of 100 ng/µl pPAL supercoiled expression vectors, chemi-competent cells, SOC growth media; sufficient for 20 reactions
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1563003
Pkg of 10 vials, 0.05 ml BL21 (DE3) E. coli cells, also includes pUC 19 control plasmid, 10 ml vial of SOC growth media
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1563002
Includes 100 μl of 100 ng/μl pPAL7 supercoiled plasmid expression vector; sufficient for 20 reactions
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1563007
Pkg of 10, mini spin columns packed with 0.1 ml Profinity eXact Resin, includes 50 capped, collection tubes
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7324648
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324647
Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324646
Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

Documents

Documents

Number Description Options
5655
Profinity eXact™ Resin Product Information Sheet, Rev A
5668
Profinity eXact Fusion-Tag System Brochure, Rev A
5646
Profinity eXact™ Cloning and Expression Kits, PIS, Rev B
5656
Purification of Tag-Free Recombinant Proteins Using the Profinity eXact Fusion-Tag System, Rev B
5652
Profinity eXact Fusion-Tag System Performs On-Column Cleavage and Yields Pure Native Protein From Lysate in Less Than an Hour, Rev A
10011163
Profinity eXact™ Mini Spin Columns Manual, Rev A
5766
Purification of Functional Heterotrimeric G-Protein a-Subunits Using the Profinity eXact Fusion-Tag System on the Profinia Protein Purification System, Rev A
5725
Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A
10011260
Profinity eXact Protein Purification System Manual, Rev A