Profinity Exact Purification Resin

Profinity eXact Purification Resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. The resulting purity of the eluted protein is typically higher than that for other affinity-tag systems due to the specific recognition (K_D < 100 pM) that subtilisin has for its prodomain sequence (the Profinity eXact Tag). Once the Profinity eXact fusion protein has been captured by the Profinity eXact Purification Resin — and the cleavage reaction has been triggered with a sodium fluoride-containing buffer — the affinity tag is precisely cleaved at the C-terminus of the cleavage recognition sequence (EEDKLFKAL). The result is a quickly purified protein product that lacks any amino acid residues derived from the Profinity eXact Tag.

Features and Benefits

• Purification and processing of fusion-tagged proteins in a single-step
• On-column cleavage in as little as 30 min
• No protease addition required
• Precise cleavage at N-terminus to generate native protein sequence

The following convenient packaging configurations allow easy evaluation of Profinity eXact technology:

• Profinity eXact Resin — 10 ml affinity resin supplied in 100 mM sodium phosphate, pH 7.2 containing 0.02% sodium azide
• Profinity eXact Mini Spin Columns — 10-pack of single-use columns that contain 0.1 ml settled resin in addition to all required collection tubes for binding, washing, and elution steps. Fit all benchtop centrifuges
• Profinity eXact Mini Spin Starter Kit — comprehensive 10-pack kit that ensures purification success upon initial trial. Includes 10 prepacked spin columns, collection tubes, lysis reagent, buffers for purification, and lyophilized MBP control protein lysate
• Bio-Scale Mini Profinity eXact Cartridges — 1 ml and 5 ml cartridges that can be used with BioLogic chromatography systems, the Profinia Protein Purification System, a peristaltic pump, or with appropriate column adaptors with any other chromatography system
• Profinity eXact Expression and Purification Starter Kit — includes two kits:
  • 1 Profinity eXact Cloning and Expression Starter Kit for the cloning and expression of a target gene using pPAL vectors and competent cells
  • 1 Profinity eXact Mini Spin Purification Starter Kit for single-step purification and on-column cleavage of the tagged protein

More Information

• Profinity eXact Resin Product Information Sheet (Bulletin 5655)
• Profinity eXact Fusion-Tag System Brochure (Bulletin 5668)
• Profinity eXact Protein Purification System Manual (Bulletin 10011260)