Profinia Protein Purification System Kits

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Overview

Profinia kits are used in a variety of protein purification methods, such as IMAC, GST, and desalting, carried out on the Profinia protein purification system.
Read full description Ordering Information

Description

Description

Profinia kits are used in a variety of protein purification methods, such as IMAC, GST, and desalting, carried out on the Profinia protein purification system. Three main kit configurations are available:

  • Profinia purification kits
  • Profinia starter kits
  • Profinia buffer kits

Profinia purification kits are supplied with a set of prepacked Bio-Scale Mini cartridges (for IMAC, GST, or desalting), application-specific purification buffers, and cleaning and storage reagents. Profinia starter kits contain a set of purification cartridges and application-specific buffers and reagents, but are also supplied with a control lysate to facilitate system setup and initial purification. Profinia buffer kits are an appropriate choice if you require only additional buffers and cleaning and storage reagents.

Profinia consumables are optimally formulated and ready to use, minimizing the time required to obtain a purified sample. Typical yields and recovery from a single purification run on a 1 ml cartridge are:

  • ≥10 mg protein in ~35 min for native IMAC
  • ≥10 mg protein in ~20 min for denaturing IMAC
  • ≥10 mg protein in ~50 min for GST

All reagents included in these kits are made with chromatography-grade solutions and have been prefiltered through a 0.2 µm filter. These reagents are specially formulated to work with the Profinia instrument. They will allow you to automatically carry out equilibration, binding, washing, and elution steps as well as necessary cartridge maintenance procedures, such as cartridge cleaning and storage.

Ordering

Ordering

items

Use the filters below to refine results!

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6200225
Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges
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6200235
Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia IMAC buffer kits (#620-0221)
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6200226
Kit for use with the Profinia protein purification system, includes Profinia GST buffer kit (#620-0223), 2 each of 1 ml GST and 10 ml desalting cartridges
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6200236
Kit for use with the Profinia protein purification system, includes 1 each of 5 ml GST and 50 ml desalting cartridges, 2 Profinia GST buffer kits (#620-0223)
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6200228
Kit for use with the Profinia protein purification system, includes Profinia desalting buffer kit (#620-0224), 2 x 10 ml desalting cartridges
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6200238
Kit for use with the Profinia protein purification system, includes 50 ml desalting cartridge, 2 Profinia desalting buffer kits (#620-0224)
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6200221
Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system
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6200223
Buffers, solutions, and glutathione sufficient for 10 applications, for use with the Profinia protein purification system
620-0224_tn.jpg
6200224
Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system
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6200229
Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 1 each of 1 ml IMAC and 10 ml desalting cartridges, E. coli lysate
620-0230_tn.jpg
6200230
Kit for use with the Profinia protein purification system, includes Profinia GST buffer kit (#620-0223), 1 each of 1 ml GST and 10 ml desalting cartridges, E. coli lysate, glutathione

Cartridges

Cartridges

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7324648
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324647
Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324646
Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7325314
Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7325312
Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7325304
Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7324602
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324600
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324624
Pkg of 5, 5 ml, prepacked affinity columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4622_tn.jpg
7324622
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4620_tn.jpg
7324620
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4614_tn.jpg
7324614
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324612
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324610
Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

Documents

Documents

Number Description Options
M10006044
Instruction Manual, Profinia Purification and Buffer Kits, Rev D
M10006057
Instruction Manual, Profinia IMAC and GST Starter Kits, Rev E
10015932
Profinia™ Protein Purification System FAQs, Rev B