Profinia Protein Purification Instrument

Image
lsr_profinia_protein_pur_instrument.jpg

Overview

Discontinued Product
The Profinia Protein Purification Intrument is no longer available. Please consider the NGC Medium-Pressure Chromatography System or the BioLogic Low-Pressure Chromatography System as alternative products.
Ordering Information

Specifications

Specifications

Operating Limits
 
Flow rate range
0.2–20 ml/min
Maximum backpressure
45 psi/3.4 bar
Operating temperature range
4–30ºC
Detection
 
UV absorbance wavelength
280 nm
Conductivity monitor range
0–500 ms/cm
pH monitor (optional) pH range
1–14
Hardware
Sample and fraction 15 or 50 ml conical tubes; can use larger external reservoirs connected by tubing
Collection containers
Buffer selection rotary selector valves for up to 8 application-specific buffers
Pressure
Sensor monitors system pressure
Solvent compatibility
All commonly used chromatographic solvents
Data
 
Output USB
Flash drive or direct USB cable to PC connection
Reporting software (optional)
Windows 2000 or XP; chromatographic data display, reporting, and analysis in real time or using stored run files
User interface
Touch screen 115 x 86 mm; select method, sample, and cartridge options by stylus or finger
Optional accessories (sold separately)
Sample and fraction cooling accessory; desalting sample loops; pH monitor; Profinia software
Physical Specifications
 
Dimensions (W x D x H)
58 x 33 x 67 cm

Ordering

Ordering

items

Use the filters below to refine results!

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6201005
Discontinued item – replaced by the NGC™ Medium-Pressure System or BioLogic™ Low-Pressure System.
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6201006
Discontinued item – replaced by the NGC™ Medium-Pressure System or BioLogic™ Low-Pressure System.
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6201010
Discontinued item – replaced by the NGC™ Medium-Pressure System or BioLogic™ Low-Pressure System.
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6201011
Discontinued item – replaced by the NGC™ Medium-Pressure System or BioLogic™ Low-Pressure System.
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6201015
100–240 V, automated chromatography system, includes Profinia instrument and software, computer, Profinia instrument accessory kit (#620-0410) and Profinia native IMAC starter kit (#620-0229)
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6201016
100–240 V, automated chromatography system, includes Profinia instrument and software, computer, Profinia instrument accessory kit (#620-0410) and Profinia GST starter kit (#620-0230)

Accessories

Accessories

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(Discontinued) Profinia Software
6200010
PC, version 2, software for monitoring and reporting data from the Profinia protein purification instrument, CD-ROM, includes USB cable
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6200401
Pkg of 2, cooling unit for sample loading and collection, for use with the Profinia protein purification instrument
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6200411
Inline pH monitor for use with the Profinia protein purification instrument, includes pH electrode, flow cell, mounting accessories
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6200402
Pkg of 1, 2 ml, desalting cartridge accessory for use with the Profinia protein purification system, includes 10 ml syringe and fittings
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6200403
Pkg of 1, 10 ml, desalting cartridge accessory for use with the Profinia protein purification system, includes 10 ml syringe and fittings
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6200404
Pkg of 12 filters, replacement filters to reduce line clogging and minimize high backpressure on the Profinia protein purification instrument
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6200405
Pkg of 10 pieces, precut 13.5 cm (5.3") polypropylene tubing for buffer and sample inlet compartments on the Profinia protein purification instrument
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6200410
Accessory kit for the Profinia protein purification instrument, includes cleaning tray, inline filter pack, sample lids, bottle starter pack, waste/diluent bottle set
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(Discontinued) Bottle Starter Pack
6200231
Pkg of 8, includes 4 x 125 ml buffer bottles, 4 x 250 ml buffer bottles, 8 buffer bottle lids; for use with the Profinia protein purification system

Kits & Reagents

Kits & Reagents

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6200225
Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges
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6200235
Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia IMAC buffer kits (#620-0221)
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6200226
Kit for use with the Profinia protein purification system, includes Profinia GST buffer kit (#620-0223), 2 each of 1 ml GST and 10 ml desalting cartridges
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6200236
Kit for use with the Profinia protein purification system, includes 1 each of 5 ml GST and 50 ml desalting cartridges, 2 Profinia GST buffer kits (#620-0223)
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6200228
Kit for use with the Profinia protein purification system, includes Profinia desalting buffer kit (#620-0224), 2 x 10 ml desalting cartridges
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6200238
Kit for use with the Profinia protein purification system, includes 50 ml desalting cartridge, 2 Profinia desalting buffer kits (#620-0224)
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6200221
Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system
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6200223
Buffers, solutions, and glutathione sufficient for 10 applications, for use with the Profinia protein purification system
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6200224
Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system
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6200229
Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 1 each of 1 ml IMAC and 10 ml desalting cartridges, E. coli lysate
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6200230
Kit for use with the Profinia protein purification system, includes Profinia GST buffer kit (#620-0223), 1 each of 1 ml GST and 10 ml desalting cartridges, E. coli lysate, glutathione
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(Discontinued) His Antibody
6200203
Pkg of 1 vial, 1 mg/ml, monoclonal antibody against histidine (His)-tagged proteins, for use with the Profinia protein purification system
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(Discontinued) GST Antibody
6200204
Pkg of 1 vial, 1 mg/ml, monoclonal antibody against GST-tagged proteins, for use with the Profinia protein purification system
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6200233
Lyophilized, dual-tagged 51 kD protein for system setup and initial purification, for use with the Profinia protein purification system
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(Discontinued) Glutathione Pack
6200202
1.23 g, powdered reagent, for denaturing GST applications on the Profinia protein purification system

Cartridges

Cartridges

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7324610
Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324612
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324614
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324620
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324622
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324624
Pkg of 5, 5 ml, prepacked affinity columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324646
Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324647
Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324648
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324600
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324602
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7325304
Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7325312
Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7325314
Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

Documents

Documents

Number Description Options
10006036
Instruction Manual, Profinia Protein Purification System, Rev F
10015932
Profinia™ Protein Purification System FAQs, Rev B
10015931
Understanding Chromatograms Generated by the Profinia Protein Purification System Reference Guide, Rev B
10006081
GST Antibody Instruction Manual, Rev C
5514
Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A
5513
Automated Purification of a GST-Tagged Protein With the Profinia Protein Purification System: Comparison to Manual Protein Purification Using Commercial Kits, Rev A
5539
Automated Desalting of Proteins With the Profinia Protein Purification System: Comparison to Manual Desalting by Dialysis, Rev A
5712
The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A
5726
Profinia Protein A and G methods Product Information Sheet, Rev A
5725
Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A
5741
Profinia Buffers for Maltose Binding Protein (MBP) Chromatography Product Information Sheet, Rev A
5742
Profinity eXact Purification Methods on the Profinia System, Rev. A
5744
Profinia Buffers for Strep-Tag Protein Chromatography Product Information Sheet, Rev A
5766
Purification of Functional Heterotrimeric G-Protein a-Subunits Using the Profinity eXact Fusion-Tag System on the Profinia Protein Purification System, Rev A
5573
The Profinia Protein Purification System: An Automated Solution for Purifying Affinity-Tagged Proteins, Rev A
5541
Profinia Protein Purification System Product Information Sheet, Rev A
5510
Profinia Protein Purification System Brochure, Rev A
5701
Profinia Buffers for Protein A and Protein G Chromatography PIS, Rev B
5926
Modular Protein Purification, Rev A