PDS-1000 | He™ System

PDS-1000 | He™ System

Overview

The PDS-1000/He system uses a burst of high-pressure helium gas to accelerate nucleic acid–coated gold or tungsten microparticles to velocities necessary to transfect cells, tissues, or organelles.

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Description

Description

PDS-1000/He™ System

The PDS-1000/He system accelerates nucleic acid–coated gold or tungsten microparticles (0.6–1.6 µm) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.

The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they travel toward the target cells

Key Features

  • A reproducible method for transforming intact cells in culture, requiring little manipulation of cells
  • Transformation of cells with unique growth requirements that are not amenable to other methods of transfection
  • Maximum control
  • High reproducibility

 

PDS-1000 / He™ and Hepta™ Training Video. This video provides an overview of the PDS-1000 / He™ and Hepta™ Biolistic Particle Transfection Systems, including instrument setup, consumable preparation, instrument operation and shut down.

Specifications

Specifications

Dimensions (W x D x H)
29 x 25.5 x 47.5 cm
Construction
Aluminum, ABS plastic, and acrylic chassis
Weight
15 kg
Electrical input voltage
100–120 VAC, 50–60 Hz
Maximum current
<5 A
Mechanical
 
Fuse
6.3 A, 250 V, 5 x 20 mm
Vacuum
<0.4 inches mercury/min leakage
Overpressure
0.5 psi relief valve, self-resetting
Environmental
 
Operating
Temperature: 0–35°C (32–95°F)
Humidity: 0–95% noncondensing
Storage
Temperature: 0–70°C (32–158°F)
Humidity: 0–95% noncondensing

Use

For Research Use Only. Not for use in diagnostic procedures.

Ordering

Ordering

items

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(Discontinued) PDS-1000/He System
1652257
100/120 V, biolistic transformation system, includes helium pressure regulator, solenoid, spacer rods, microcarrier launch assembly, target shelf, 5 macrocarrier holders, tubing

Accessories

Accessories

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(Discontinued) 500 Optimization Kit
1652278

Pkg of 1, optimization kit for the PDS-1000/He system, includes 0.25 g each of 0.6 μm, 1.0 μm, and 1.6 μm gold microcarriers, 100 each of 7 rupture disks, for 500 bombardments

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1652326
Pkg of 100, bursts at 450 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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1652327
Pkg of 100, bursts at 650 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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1652328
Pkg of 100, bursts at 900 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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1652329
Pkg of 100, bursts at 1,100 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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1652330
Pkg of 100, bursts at 1,350 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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1652331
Pkg of 100, bursts at 1,550 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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1652332
Pkg of 100, bursts at 1,800 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)

Documents

Documents

Number Description