PDS-1000 | He™ and Hepta™ Systems

Image
lsr_pds1000.jpg

Overview

The PDS-1000/He system uses a burst of high-pressure helium gas to accelerate nucleic acid–coated gold or tungsten microparticles to velocities necessary to transfect cells, tissues, or organelles.
Read full description Ordering Information

Description

Description

PDS-1000/He™ System

The PDS-1000/He system accelerates nucleic acid–coated gold or tungsten microparticles (0.6–1.6 µm) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.

The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they travel toward the target cells

Key Features

    • A reproducible method for transforming intact cells in culture, requiring little manipulation of cells
    • Transformation of cells with unique growth requirements that are not amenable to other methods of transfection
    • Maximum control
    • High reproducibility

PDS-1000/He System with Hepta™ Adaptor

The Hepta adaptor for the PDS-1000/He biolistic system enables 7–10 times more cells to be transformed than the standard system. It fits into the shocking chamber of the PDS-1000/He system where it splits the helium shock wave over seven macrocarriers. By uniformly spreading the DNA-coated gold or tungsten particles over this larger area, the system maximizes the number of cells transformed during one bombardment. As the helium is split seven ways, pressure and particle velocities are reduced, making the system ideal for plants and cell cultures requiring less forceful penetration.


PDS-1000 / He™ and Hepta™ Training Video. This video provides an overview of the PDS-1000 / He™ and Hepta™ Biolistic Particle Transfection Systems, including instrument setup, consumable preparation, instrument operation and shut down.

Specifications

Specifications

Dimensions (W x D x H)
29 x 25.5 x 47.5 cm
Construction
Aluminum, ABS plastic, and acrylic chassis
Weight
15 kg
Electrical input voltage
100–120 VAC, 50–60 Hz
Maximum current
<5 A
Mechanical
 
Fuse
6.3 A, 250 V, 5 x 20 mm
Vacuum
<0.4 inches mercury/min leakage
Overpressure
0.5 psi relief valve, self-resetting
Environmental
 
Operating
Temperature: 0–35°C (32–95°F)
Humidity: 0–95% noncondensing
Storage
Temperature: 0–70°C (32–158°F)
Humidity: 0–95% noncondensing

Ordering

Ordering

items

Use the filters below to refine results!

165-2257_tn.jpg
(Discontinued) PDS-1000/He System
1652257
100/120 V, biolistic transformation system, includes helium pressure regulator, solenoid, spacer rods, microcarrier launch assembly, target shelf, 5 macrocarrier holders, tubing
165-2258_tn.jpg
1652258
100/120 V, biolistic transformation system, includes Hepta adaptor, helium pressure regulator, solenoid, spacer rods, microcarrier launch assembly, target shelf, 5 macrocarrier holders, tubing
165-2225_tn.jpg
1652225
Pkg of 1, biolistic shock wave splitter for use with the PDS-1000/He system (#165-2257), includes 5 stopping screens
165-2259_tn.jpg
(Discontinued) Voltage Converter
1652259
Pkg of 1, voltage converter, for use with PDS-1000/He system on 220–240 V lines

Accessories

Accessories

165-2278_tn.jpg
(Discontinued) 500 Optimization Kit
1652278
Pkg of 1, optimization kit for the PDS-1000/He system, includes 0.25 g each of 0.6 μm, 1.0 μm, and 1.6 μm gold microcarriers, 100 each of 9 rupture disks, for 500 bombardments
165-2335_tn.jpg
(Discontinued) Macrocarriers
1652335
Pkg of 500, microparticles carrier disk, for use with PDS-1000/He and Hepta systems (#165-2257, #165-2258)
165-2322_tn.jpg
(Discontinued) Macrocarrier Holders
1652322
Pkg of 5, reusable part, for use with the PDS-1000/He biolistic systems (#165-2257, #165-2258)
165-2326_tn.jpg
1652326
Pkg of 100, bursts at 450 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
165-2327_tn.jpg
1652327
Pkg of 100, bursts at 650 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
165-2328_tn.jpg
1652328
Pkg of 100, bursts at 900 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
165-2329_tn.jpg
1652329
Pkg of 100, bursts at 1,100 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
165-2330_tn.jpg
1652330
Pkg of 100, bursts at 1,350 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
165-2331_tn.jpg
1652331
Pkg of 100, bursts at 1,550 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
165-2332_tn.jpg
1652332
Pkg of 100, bursts at 1,800 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
165-2333_tn.jpg
1652333
Pkg of 100, bursts at 2,000 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
165-2334_tn.jpg
1652334
Pkg of 100, bursts at 2,200 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
165-2336_tn.jpg
(Discontinued) Stopping Screens
1652336
Pkg of 500, for use with the PDS-1000/He system (#165-2257)
165-2226_tn.jpg
1652226
Pkg of 50, stopping screens for use with the Hepta adaptor of the PDS-1000/He systems (#165-2257, #165-2258)
165-2262_tn.jpg
1652262
0.25 g, 0.6 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
165-2263_tn.jpg
1652263
0.25 g, 1.0 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
165-2264_tn.jpg
1652264
0.25 g, 1.6 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
165-2266_tn.jpg
1652266
6 g, ~0.7 μm diameter, tungsten microcarriers for use with the PDS-1000/He system
165-2267_tn.jpg
1652267
6 g, ~1.1 μm diameter, tungsten microcarriers for use with the PDS-1000/He system
165-2268_tn.jpg
1652268
6 g, ~1.3 μm diameter, tungsten microcarriers for use with the PDS-1000/He system
165-2269_tn.jpg
1652269
6 g, ~1.7 μm diameter, tungsten microcarriers for use with the PDS-1000/He system

Documents

Documents

Number Description Options
5443
Biolistic Particle Delivery Systems Brochure, Rev A
5447
Biolistic PDS-1000/He System Flier, Rev A
10000070900
Instruction Manual, PDS-1000/He Biolistic Particle Delivery System
4100150
Instruction Manual, Safety Addendum for the Biolistic PDS-1000/He Particle Delivery System, Rev A
4106154
Instruction Manual, Hepta Adaptor, PDS-1000/He Biolistic Particle Delivery System, Rev A
2015
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cell Types
2007
Bombardment-Mediated Transformation Methods for Barley
2051
Transformation of Filamentous Fungi by Microprojectile Bombardment
2658
Single-Cell Complementation of Barley mlo Mutants Using a PDS-1000/He Hepta System
2087
Biolistic Transfection of Organotypic Brain Slices and Dissociated Cells
2453
Optimization of Gene Delivery Into Arabidopsis, Tobacco, and Birch Using the Helios Gene Gun System
1688
Optimization of Biolistic® Transformation Using the Helium-Driven PDS-1000/He System
1689
Comparison of Performance Characteristics of Different Biolistic® Devices
2768
Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A