Nuvia™ IMAC Metal Affinity Resin

Image
nuvia-imac-resin-detail.jpg

Overview

 Nuvia IMAC (immobilized metal affinity chromatography) Resin provides an ultra-high capacity matrix over a wide range of flow rates and pH for process scale chromatography. Get reproducible separation of biomolecules free of contaminants.
Read full description Ordering Information

Description

Description

Nuvia IMAC Resin is a metal chelate affinity chromatography resin that contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The inert hydrophilic surface of the base bead and the chemical structure of NTA ensure highly selective binding of recombinant histidine-tagged proteins when charged with Ni2+, or with other transition metals such as Zn2+ or Cu2+. The optimized particle size and high porosity allows operation at increasingly higher flow rates without compromising binding capacity. Nuvia IMAC can be used at any scale from laboratory to manufacturing, providing robust, reproducible, and efficient purifications each time.

Nuvia IMAC Resin is stable in a broad range of chemicals. It is compatible with all reagents traditionally used for histidine-tagged protein purification, including reducing agents, detergents, denaturing agents, and additives.

Features and Benefits

  • Easy and efficient scale up from lab to manufacturing scale purifications
  • Chelating ligand nitrilotriacetic acid (NTA) may be charged with transition metals such as Cu2+, Ni2+, Co2+, or Zn2+
  • Optimized particle size for high flow rate and low backpressure
  • Superior dynamic binding capacity at high flow rates
  • Withstands repeated clean-in-place/recharging cycles
  • Robust recovery and reproducibility
  • Full regulatory support

Applications and Uses of Nuvia IMAC Resin

For purifying biomolecules in a variety of workflows:

  • Excellent single-step purity via affinity chromatography
  • Quick, efficient separation of biomolecules
  • Contaminant removal
  • Robust base matrix

Nuvia IMAC Resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.

Product Options

Package Size Catalog Number
Nuvia IMAC Ni-Charged Support, bulk resin  
25 ml 7800800
100 ml 7800801
500 ml 7800802
5 L 12003233
10 L 12002782
Nuvia IMAC Ni-Charged Support, prepacked  
Prepacked column, 1 ml 12004038
Prepacked column, 5 ml 12004037
Prepacked filter plates, 20 µl 12004035
Prepacked RoboColumns, 200 µl 12004051
Prepacked RoboColumns, 600 µl 12004052
Nuvia IMAC Uncharged Support, bulk resin  
5 L 12004040
10 L 12004039
 

More Information

Download the Nuvia IMAC Resin Instruction Manual for detailed column packing recommendations and protocols for optimal protein isolation and column regeneration, cleaning, sanitizing, and storage. Prepacked Bio-Scale™ Mini Nuvia IMAC Cartridges with Ni-charged NTA are available.

Specifications

Specifications

Particle size
38–53 µm
Ligand density
≥ 18 µmol/ml
Dynamic binding capacity*
> 40 mg/ml at 300 cm/hr
*10% breakthrough capacity determined with 1.2 mg/ml of a 40 kD histidine-tagged protein in 50 mM sodium phosphate, 5 mM imidazole, 300 mM NaCl (pH 7.5).
Compression factor
1.20–1.25
Recommended linear flow rate range
50–300 cm/hr
Pressure vs. flow performance
Under 2 bar at flow rate of 300 cm/hr in DI water (20 x 20 cm packed bed, 1.2 compression factor)
pH stability
2–14
Shipping solution
2% benzyl alcohol or 20% ethanol
Regeneration
50 mM EDTA, pH 8.0 (Stripping)
1N NaOH (CIP/SIP)
0.1 M Ni2SO4 (Recharging)
Cleaning-in-place (CIP) solutions
1N NaOH
Sanitization
1N NaOH
Storage condition
20% Ethanol
Chemical compatibility
Reducing agents (β-ME, TCEP, DTT)
Denaturing agents (GnHCl)
Detergents (Triton X-100, NP-40, CHAPS, CHAPSO)
Additives (glycerol)
**No recharge required. Refer to the instruction manual for a complete list.
Chemical stability
48 hr with 1M NaOH, 1 week with 0.01M HCl
***Recharge required. Refer to the instruction manual for a complete list.

Ordering

Ordering

items

Use the filters below to refine results!

nuvia-imac-nicharged-resin-780-0800-thumb.jpg
7800800
Pkg of 1, 25 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
nuvia-imac-nicharged-resin-780-0801-thumb.jpg
7800801
Pkg of 1, 100 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
nuvia-imac-nicharged-resin-780-0802-thumb.jpg
7800802
Pkg of 1, 500 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
12004040-nuvia-imac-uncharged-resin-5-l-tn.jpg
12003233
Pkg of 1, 5 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
12004039-nuvia-imac-uncharged-resin-10-l-tn.jpg
12002782
Pkg of 1, 10 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
12004040-nuvia-imac-uncharged-resin-5-l-tn.jpg
12004040
Pkg of 1, 5 L, Nuvia IMAC Support with uncharged nitrilotriacetic acid (NTA) for immobilized metal affinity chromatography
12004039-nuvia-imac-uncharged-resin-10-l-tn.jpg
12004039
Pkg of 1, 10 L, Nuvia IMAC Support with uncharged nitrilotriacetic acid (NTA) for immobilized metal affinity chromatography
12004038-foresight-nuvia-ni-charged-imac-1-ml-column-tn.jpg
12004038
Pkg of 1, 1 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin
12004037-foresight-nuvia-ni-charged-imac-5-ml-column-tn.jpg
12004037
Pkg of 1, 5 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin
12004035-foresight-nuvia-ni-charged-imac-filter-plates-20-ul-tn.jpg
12004035
2 x 96-well plates, 20 µl, filter plate prepacked with Nuvia IMAC Affinity Chromatography Resin
12004051-foresight-nuvia-ni-charged-imac-robocolumn-unit-200-ul-tn.jpg
12004051
1 row of 8 columns, 200 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin
12004052-foresight-nuvia-foresight-nuvia-ni-charged-imac-robocolumn-unit-600-ul-tn.jpg
12004052
1 row of 8 columns, 600 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin
bio-scale-mini-nuvia-imac-nicharged-column-780-0811-thumb.jpg
7800811
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system
bio-scale-mini-nuvia-imac-nicharged-columns-780-0812-thumb.jpg
7800812
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system

Documents

Documents

Number Description Options
D116698
Instruction Manual, Nuvia™ IMAC Resin
6859
Nuvia™ IMAC Affinity Chromatography Resin Product Information Sheet, Ver B
10041106
Instruction Manual, Bio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges, 1 and 5 ml, Rev A
10042131
Instruction Manual, Bio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges, 1 and 5 ml Quick Start Guide, Rev B
10024831
Instruction Manual, Foresight™ Chromatography Media Columns 1 and 5 ml, Rev A
10027565
Instruction Manual, Foresight™ RoboColumn Units 200 and 600 µl, Rev A
10024413
Instruction Manual, Foresight™ Chromatography Media Filter Plates, Rev A
6297
Foresight Prepacked Plates and Columns Product Information Sheet
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6860
A Scalable Immobilized Metal Affinity Chromatography Resin for Process Purification
6769
From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification (Poster), Ver A
6964
A Nuvia IMAC Resin–Based Purification Workflow for the Production of Highly Pure Recombinant Proteins
7028
Nuvia IMAC Packing Protocol Application Note, Ver A