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Description
Description
Nuvia IMAC Resin is a metal chelate affinity chromatography resin that contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The inert hydrophilic surface of the base bead and the chemical structure of NTA ensure highly selective binding of recombinant histidine-tagged proteins when charged with Ni2+, or with other transition metals such as Zn2+ or Cu2+. The optimized particle size and high porosity allows operation at increasingly higher flow rates without compromising binding capacity. Nuvia IMAC can be used at any scale from laboratory to manufacturing, providing robust, reproducible, and efficient purifications each time.
Nuvia IMAC Resin is stable in a broad range of chemicals. It is compatible with all reagents traditionally used for histidine-tagged protein purification, including reducing agents, detergents, denaturing agents, and additives.
Features and Benefits
- Easy and efficient scale up from lab to manufacturing scale purifications
- Chelating ligand nitrilotriacetic acid (NTA) may be charged with transition metals such as Cu2+, Ni2+, Co2+, or Zn2+
- Optimized particle size for high flow rate and low backpressure
- Superior dynamic binding capacity at high flow rates
- Withstands repeated clean-in-place/recharging cycles
- Robust recovery and reproducibility
- Full regulatory support
Applications and Uses of Nuvia IMAC Resin
For purifying biomolecules in a variety of workflows:
- Excellent single-step purity via affinity chromatography
- Quick, efficient separation of biomolecules
- Contaminant removal
- Robust base matrix
Nuvia IMAC Resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.
Product Options
| Package Size | Catalog Number |
| Nuvia IMAC Ni-Charged Support, bulk resin | |
| 25 ml | 7800800 |
| 100 ml | 7800801 |
| 500 ml | 7800802 |
| 5 L | 12003233 |
| 10 L | 12002782 |
| Nuvia IMAC Ni-Charged Support, prepacked | |
| Prepacked column, 1 ml | 12004038 |
| Prepacked column, 5 ml | 12004037 |
| Prepacked filter plates, 20 µl | 12004035 |
| Prepacked RoboColumns, 200 µl | 12004051 |
| Prepacked RoboColumns, 600 µl | 12004052 |
| Nuvia IMAC Uncharged Support, bulk resin | |
| 5 L | 12004040 |
| 10 L | 12004039 |
More Information
Download the Nuvia IMAC Resin Instruction Manual for detailed column packing recommendations and protocols for optimal protein isolation and column regeneration, cleaning, sanitizing, and storage. Prepacked Bio-Scale™ Mini Nuvia IMAC Cartridges with Ni-charged NTA are available.
Specifications
Specifications
Particle size
38–53 µm
Ligand density
≥ 18 µmol/ml
Dynamic binding capacity*
> 40 mg/ml at 300 cm/hr
*10% breakthrough capacity determined with 1.2 mg/ml of a 40 kD histidine-tagged protein in 50 mM sodium phosphate, 5 mM imidazole, 300 mM NaCl (pH 7.5).
*10% breakthrough capacity determined with 1.2 mg/ml of a 40 kD histidine-tagged protein in 50 mM sodium phosphate, 5 mM imidazole, 300 mM NaCl (pH 7.5).
Compression factor
1.20–1.25
Recommended linear flow rate range
50–300 cm/hr
Pressure vs. flow performance
Under 2 bar at flow rate of 300 cm/hr in DI water (20 x 20 cm packed bed, 1.2 compression factor)
pH stability
2–14
Shipping solution
2% benzyl alcohol or 20% ethanol
Regeneration
50 mM EDTA, pH 8.0 (Stripping)
1N NaOH (CIP/SIP)
0.1 M Ni2SO4 (Recharging)
1N NaOH (CIP/SIP)
0.1 M Ni2SO4 (Recharging)
Cleaning-in-place (CIP) solutions
1N NaOH
Sanitization
1N NaOH
Storage condition
20% Ethanol
Chemical compatibility
Reducing agents (β-ME, TCEP, DTT)
Denaturing agents (GnHCl)
Detergents (Triton X-100, NP-40, CHAPS, CHAPSO)
Additives (glycerol)
**No recharge required. Refer to the instruction manual for a complete list.
Denaturing agents (GnHCl)
Detergents (Triton X-100, NP-40, CHAPS, CHAPSO)
Additives (glycerol)
**No recharge required. Refer to the instruction manual for a complete list.
Chemical stability
48 hr with 1M NaOH, 1 week with 0.01M HCl
***Recharge required. Refer to the instruction manual for a complete list.
***Recharge required. Refer to the instruction manual for a complete list.
Ordering
Ordering
items
Use the filters below to refine results!
(Discontinued) Nuvia™ IMAC Resin, 25 ml
7800800
Pkg of 1, 25 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
(Discontinued) Nuvia™ IMAC Resin, 100 ml
7800801
Pkg of 1, 100 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
(Discontinued) Nuvia™ IMAC Resin, 500 ml
7800802
Pkg of 1, 500 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
(Discontinued) Nuvia™ IMAC Resin, 5 L
12003233
Pkg of 1, 5 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
(Discontinued) Nuvia™ IMAC Resin, 10 L
12002782
Pkg of 1, 10 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography
(Discontinued) Nuvia™ IMAC Uncharged Resin, 5 L
12004040
Pkg of 1, 5 L, Nuvia IMAC Support with uncharged nitrilotriacetic acid (NTA) for immobilized metal affinity chromatography
(Discontinued) Nuvia™ IMAC Uncharged Resin, 10 L
12004039
Pkg of 1, 10 L, Nuvia IMAC Support with uncharged nitrilotriacetic acid (NTA) for immobilized metal affinity chromatography
(Discontinued) Foresight™ Nuvia™ Ni-Charged IMAC, 1 ml column
12004038
Pkg of 1, 1 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin
(Discontinued) Foresight™ Nuvia™ Ni-Charged IMAC, 5 ml column
12004037
Pkg of 1, 5 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin
(Discontinued) Foresight™ Nuvia™ Ni-Charged IMAC Filter Plates, 20 µl
12004035
2 x 96-well plates, 20 µl, filter plate prepacked with Nuvia IMAC Affinity Chromatography Resin
(Discontinued) Foresight™ Nuvia™ Ni-Charged IMAC RoboColumn Unit, 200 µl
12004051
1 row of 8 columns, 200 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin
(Discontinued) Foresight™ Nuvia™ Ni-Charged IMAC RoboColumn Unit, 600 µl
12004052
1 row of 8 columns, 600 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin
(Discontinued) Bio-Scale™ Mini Nuvia™ IMAC Cartridges
7800811
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system
(Discontinued) Bio-Scale™ Mini Nuvia™ IMAC Cartridges
7800812
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system
Documents
Documents
Login Required Number Description Options
D116698
Instruction Manual, Nuvia™ IMAC Resin
6859
Nuvia™ IMAC Affinity Chromatography Resin Product Information Sheet, Ver B
10041106
Instruction Manual, Bio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges, 1 and 5 ml, Rev A
10042131
Instruction Manual, Bio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges, 1 and 5 ml Quick Start Guide, Rev B
10024831
Instruction Manual, Foresight™ Chromatography Media Columns 1 and 5 ml, Rev A
10027565
Instruction Manual, Foresight™ RoboColumn Units 200 and 600 µl, Rev A
10024413
Instruction Manual, Foresight™ Chromatography Media Filter Plates, Rev A
6297
Foresight Prepacked Plates and Columns Product Information Sheet
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6860
A Scalable Immobilized Metal Affinity Chromatography Resin for Process Purification
6769
From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification (Poster), Ver A
6964
A Nuvia IMAC Resin–Based Purification Workflow for the Production of Highly Pure Recombinant Proteins
7028
Nuvia IMAC Packing Protocol Application Note, Ver A
