Nuvia HR-S Resin

Nuvia HR-S Resin

Overview

Nuvia HR-S Resin is a small-particle-size, high-resolution cation exchange resin designed for intermediate and final polishing purification steps where the separation of closely related biomolecules is challenging.

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Description

Description

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Nuvia HR-S Resin is a high-resolution cation exchange resin designed for intermediate and final polishing purification steps where separating closely related product impurities or charged variants presents a challenge for other chromatography resins. The exceptional selectivity and high recovery of Nuvia HR-S Resin is due to optimized particle size and chemistry, designed to meet biomolecule purification needs at both the laboratory scale and large bioprocess-scale levels.

Nuvia HR-S Resin joins the family of Nuvia S and Nuvia Q Ion Exchange Resins, and may be used for the separation of proteins, nucleic acids, viruses, plasmids, and other macromolecules. The unique properties of Nuvia HR-S Resin, including smaller particle size and fast mass transfer, allow this cation exchange resin to be particularly effective for the resolution of various biomolecules.

Nuvia HR-S Resin delivers value by allowing users to:

  • Obtain purer product from sharper elution profiles
  • Achieve higher yields, reducing cost of goods
  • Maintain robustness through consistent results even with variable initial feed conditions
  • Benefit from chemical stability that withstands repetitive clean-in-place cycles
  • Obtain full support for regulatory submission

More Information

Specifications

Specifications

Type of ion exchanger
Strong cation
Functional group
–SO3
Total ionic capacity
100–180 µeq/ml
Dynamic binding capacity*
>70 mg/ml at 300 cm/hr
Shipping counterion
Na+
Median particle size
50 ± 10 µm
Recommended linear flow rate range
50–200 cm/hr
Chemical stability
 
   1.0 N NaOH (20°C)
up to 5 weeks
   0.1 N NaOH (20°C)**
up to 5 years
Compression factor
1.25 (settled bed volume/packed bed volume)
pH stability**
 
   Short term
2–14
   Long term
4–13
Shipping solution
20% ethanol or 2% benzyl alcohol (BA)
Regeneration
1–2 M NaCl
Sanitization
0.5–1.0 N NaOH
Storage conditions
20% ethanol or 0.1 N NaOH
 
*10% breakthrough capacity determined with 5.0 mg/ml human IgG in 20 mM NaOAc pH 5.0.
 
** Data derived under accelerated conditions at 60°C.

Ordering

Ordering

items

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12003712

25 ml, strong cation exchange chromatography media, 50 µm particle size, shipped in 2% benzyl alcohol

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12003713

100 ml, strong cation exchange chromatography media, 50 µm particle size, shipped in 2% benzyl alcohol

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7324707
2 x 96-well plates, 20 µl, filter plate prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin
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7324831
1 row of 8 columns, 200 µl, RoboColumn unit prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin
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7324832
1 row of 8 columns, 600 µl, RoboColumn unit prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin
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7324723
Pkg of 1, 1 ml, column prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin
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7324743
Pkg of 1, 5 ml, column prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin
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12009284

Pkg of 1, 1 ml, prepacked cation exchange column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system

Documents

Documents

Number Description Options
7255
Investigating the Important Parameters for Packing Nuvia HR-S Resin at Process Scale
7027
Minimize On-Column Aggregates During mAb Purification
7000
Minimizing On-Column Monoclonal Antibody Aggregate Formation
6448
Nuvia HR-S Cation Exchange Media Exceptional Resolution of Closely Related Species for Process Purification, Rev A
6439
Improving Aggregrate Removal from a Monoclonal Antibody Feedstream Using High-Resolution Cation Exchange Chromatography, Rev B
10033316
Instruction Manual, Nuvia HR-S Strong Cation Exchange Media, Rev A
6875
Practical Guide: Selecting the Optimal Resins for Monoclonal Antibody Process Purification, Ver A
6808
Practical Guide: Selecting the Optimal Resins for Aggregate Removal, Ver A
6790
Practical Guide: Selecting the Optimal Resins for Mammalian Virus Process Purification and Clearance, Ver A
6786
Monoclonal Antibody Purification Resin Selection Card, Ver A
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6792
Aggregate Purification Resin Selection Card, Ver A
6862
Monoclonal Antibody Purification: Intermediate Purification Resins Brochure, Ver A
6888
Quick Monoclonal Antibody Purification Strategy: Resolving Charge Variants Flier , Ver A