Nuvia HP-Q Resin

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Overview

Nuvia HP-Q Anion Exchange Resin is a high-capacity ion exchange resin that can be used for downstream purification of large molecules such as high molecular weight (HMW) plasma proteins IgA and IgM, viruses, VLPs, and PEGylated proteins.

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Description

Description

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Nuvia HP-Q is a high-performance strong anion exchange resin. It is the latest product in the Nuvia family of high-capacity ion exchange resins and can be used for downstream purification of large molecules such as high molecular weight (HMW) plasma proteins IgA and IgM, viruses, VLPs, and PEGylated proteins. The stability of this base bead and its broad chemical compatibility allows repeated uses with a long resin lifetime. Nuvia HP-Q particle size is designed to offer high dynamic binding capacity (DBC) at fast flow rates without excessive backpressure, thereby delivering excellent process economics. Its pore size is optimized for easy accessibility and adsorption of large biomolecules, and the internal spacer length and ligand density facilitate efficient binding of the biomolecules even at high flow rates.

Features and Benefits

  • Ideal for purification of large biomolecules
  • Excellent pressure flow properties
  • Recover more target biomolecule with less resin
  • Increase productivity and decrease cycle time with high flow rates
  • Easily scalable to meet manufacturing demands
  • Improve overall process economics

Large Molecule Applications

  • Plasma proteins IgA and IgM
  • Viruses
  • VLPs
  • PEGylated proteins

Superior DBC of Nuvia HP-Q at a High Flow Rate Relative to Other Commercially Available Resins.

IgM obtained from plasma fractionation showed DBC in the range of 20-25 mg/ml with Nuvia HP-Q Resin. When compared to other commercially available resins, Nuvia HP-Q Resin demonstrated higher DBC at faster flow rates.  

Vendor/Product Matrix Material Particle Size, µm Pressure, bar Recommended Flow Rate, cm/hr1 DBC (IgM)
Nuvia HP-Q UNOsphere 50 <3 300 +++2
Resin 1 Dextran beads 50 <3 30 +++2
Resin 2 Agarose 75 <3 300 +3
Resin 3 PS/DVB 50 <3 300 +2
Resin 4 PMMA 50 <2 300 +2

1 Recommended flow rate for industrial scale column (D > 30 cm).
2 DBC data at 10% breakthrough
3 Data obtained from vendor presentation.

Product Formats

Product Catalog Number
Nuvia HP-Q Media, 25 ml 12006693
Nuvia HP-Q Media, 100 ml 12006691
Nuvia HP-Q Media, 500 ml 12006660
Nuvia HP-Q Media, 5 L 12006659
Nuvia HP-Q Media, 10 L 12007023

Screening Tools

  • Foresight CHT XT Column, 1 ml

    Foresight™ Nuvia HP-Q Column, 1 ml

    12007020

  • Foresight CHT XT Column, 5 ml

    Foresight Nuvia HP-Q Column, 5 ml

    12007021

  • Foresight CHT XT, 200 μl Robocolumn

    Foresight Nuvia HP-Q RoboColumn Unit, 200 μl

    12007013

  • Foresight CHT XT, 600 μl Robocolumn

    Foresight Nuvia HP-Q RoboColumn Unit, 600 μl

    12007014

  • Foresight CHT XT, 20 μl Plates

    Foresight Nuvia HP-Q Plates, 20 μl

    12006908

More Information

Specifications

Specifications

Property
Value
Type of ion exchanger
Strong anion
Functional group
–N+(CH3)3
Particle size range
38–53 μm
Dynamic binding capacity*
>50 mg/ml at 100 cm/hr
Recommended linear flow rate
50–300 cm/hr
Maximum operating pressure
3 bar
Compression factor
~1.2
Shipping solution
20% ethanol or
2% benzyl alcohol
Regeneration
1–2 M NaCl
Sanitization
1 N NaOH
Storage conditions
20% ethanol or 0.01 N NaOH
Storage temperature
Room temperature
Chemical stability
1 N NaOH (20°C), up to 1 week
pH stability**
 
   Long term**
2–11
Shelf life
5 years

Ordering

Ordering

items

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12006693-tn.jpg
12006693
25 ml, strong anion exchange chromatography media, 50 µm particle size
12006691_tn.jpg
12006691
100 ml, strong anion exchange chromatography media, 50 µm particle size
12006660-tn.jpg
12006660
500 ml, strong anion exchange chromatography media, 50 µm particle size
12006659-tn.jpg
12006659
5 L, strong anion exchange chromatography media, 50 µm particle size
12007023-tn.jpg
12007023
10 L, strong anion exchange chromatography media, 50 µm particle size
12007020-tn.jpg
12007020
1 ml, column prepacked with Nuvia HP-Q Strong Anion Exchange Chromatography Media, 50 µm particle size
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12007021
Pkg of 1, 5 ml, column prepacked with Nuvia HP-Q Strong Anion Exchange Chromatography Media, 50 µm particle size
12007013-th.jpg
12007013
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with Nuvia HP-Q Strong Anion Exchange Chromatography Media, 50 µm particle size
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12007014
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with Nuvia HP-Q Strong Anion Exchange Chromatography Media, 50 µm particle size
12006908-tn.jpg
12006908
2 x 96-well plates, 20 μl, filter plate prepacked with Nuvia HP-Q Strong Anion Exchange Chromatography Media, 50 µm particle size

Documents

Documents

Number Description Options
7112
Acidic IgM Purification with the Strong Anion Exchanger, Nuvia-HP-Q Resin (Poster)
7078
Nuvia™ HP-Q Anion Exchange Resin Product Information Sheet, Ver B
7098
Improve Large Biomolecule Purification Productivity and Speed
[ Add to Cart (Free) ]
10000097131
Instruction Manual, Nuvia™ HP-Q Strong Anion Exchange Media
7127
Purification of an Acidic IgM with a Strong Anion Exchange Resin Tailored for Large Biomolecule Purification
[ Add to Cart (Free) ]
7375
Quick Virus Purification Strategy: H1N1 Purification