Mini-PROTEAN® TGX™ Precast Gels

Mini-PROTEAN® TGX™ Precast Gels

Mini-PROTEAN TGX precast gels feature long shelf life and superior resolution in standard Tris/glycine PAGE buffers, fast run times, and high transfer efficiency. Available in six polyacrylamide gel percentages and two pack sizes.

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Ordering Information

Overview

Long shelf life Mini-PROTEAN® TGX™ Precast Gels are an innovative PAGE system designed to provide very fast separation while maintaining high resolution in standard Tris-glycine buffer. Mini-PROTEAN Precast Gels are compatible with Mini-PROTEAN Tetra (1–4 gels) and Mini-PROTEAN® Dodeca™ (1–12 gels) Cells. These gels can also be used in the discontinued Mini-PROTEAN 3 Cell.

Features and Benefits of Mini-PROTEAN TGX Precast Gels

  • 12 month shelf life
  • Electrophoresis run times in as short as 15 min
  • Numbered, marked well outlines on the cassette ease sample loading and identification
  • Inexpensive buffer system means low running costs
  • Bottom-open cassette design allows easy gel handling and faster blotting setup
  • Uses same buffers and band patterns as Laemmli systems

Applications and Uses of Mini-PROTEAN TGX Precast Gels

  • 1-D polyacrylamide gel electrophoresis for protein separation
  • Accurate molecular weight estimation
  • Robust system for complex samples

Mini-PROTEAN TGX Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.

Mini-PROTEAN TGX Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.

More Information

Use the the Gel Selector to find the best precast gel for your application.

Specifications

Gel dimensions (W x L x thickness), cm
8.6 x 6.7 x 0.1
Cassette dimensions (W x L x thickness), cm
10 x 8 x 0.46
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Gel storage conditions
Store flat at 2–8°C; do not freeze
Shelf life at recommended temperature*
12 months
Recommended sample buffer (Laemmli, dilute 1:1 with sample)
62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/glycine/SDS)
25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
  Gel chemistry
TGX
Opening lever
100% aluminum and recyclable
* From date of manufacture.
 

Ordering

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4561021

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561023

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561023DC

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561024

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561024DC

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561025

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561025DC

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561026

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561026DC

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561031

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561033

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561033DC

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561034

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561034DC

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561035

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561036

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561036DC

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561041

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561043

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561043DC

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561044

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561044DC

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561045

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561045DC

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561046

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561046DC

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561081

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561083

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561084

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561084DC

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561085

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561085DC

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561086

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561086DC

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561091

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561093

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561093DC

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561094

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561095

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561096

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4561101

Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561103

Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561104

Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561105

Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4561106

Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4569031

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4569033

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4569033DC

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4569034

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4569034DC

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4569035

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4569035DC

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

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4569036

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4569036DC

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

Accessories

Kits & Reagents

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1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610734

Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
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1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications
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1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
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1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications
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1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610480

Kit for silver staining polyacrylamide gels with the large Dodeca Stainer (1653400), for 12 large gels

Documents

Number Description Options
10026447
Mini-PROTEAN® TGX™ Precast Gel Quick Start Guide, Rev C
5871
Mini-PROTEAN® TGX™ Precast Gels Product Information Sheet, Rev F
[ Add to Cart (Free) ]
6040
Electrophoresis Guide, Interactive PDF, Rev C
5932
Ready Gel to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev E
5934
NuPAGE Bis-Tris Precast Gels (MOPS Buffer) to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev G
5910
Mini-PROTEAN TGX Precast Gel: A Gel for SDS-PAGE with Improved Stability — Comparison with Standard Laemmli Gels, Rev B
5911
Mini-PROTEAN TGX Precast Gel: A Versatile and Robust Laemmli-Like Precast Gel for SDS-PAGE, Rev A.
6148
Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B
6147
Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B
6507
TGX and TGX Stain-Free Gels: Publications List