Mini-PROTEAN® TGX™ Precast Gels

Description

Description

Long shelf life Mini-PROTEAN® TGX™ Precast Gels are an innovative PAGE system designed to provide very fast separation while maintaining high resolution in standard Tris-glycine buffer. Mini-PROTEAN Precast Gels are compatible with Mini-PROTEAN Tetra (1–4 gels) and Mini-PROTEAN® Dodeca™ (1–12 gels) Cells. These gels can also be used in the discontinued Mini-PROTEAN 3 Cell.

Features and Benefits of Mini-PROTEAN TGX Precast Gels

  • 12 month shelf life
  • Electrophoresis run times in as short as 15 min
  • Numbered, marked well outlines on the cassette ease sample loading and identification
  • Inexpensive buffer system means low running costs
  • Bottom-open cassette design allows easy gel handling and faster blotting setup
  • Uses same buffers and band patterns as Laemmli systems

Applications and Uses of Mini-PROTEAN TGX Precast Gels

  • 1-D polyacrylamide gel electrophoresis for protein separation
  • Accurate molecular weight estimation
  • Robust system for complex samples

Mini-PROTEAN TGX Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.

Mini-PROTEAN TGX Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.

More Information

Use the the Gel Selector to find the best precast gel for your application.

Specifications

Specifications

Gel dimensions (W x L x thickness), cm
8.6 x 6.7 x 0.1
Cassette dimensions (W x L x thickness), cm
10 x 8 x 0.46
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Gel storage conditions
Store flat at 2–8°C; do not freeze
Shelf life at recommended temperature*
12 months
Recommended sample buffer (Laemmli, dilute 1:1 with sample)
62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/glycine/SDS)
25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
  Gel chemistry
TGX
Opening lever
100% aluminum and recyclable
* From date of manufacture.
 

Ordering

Ordering

items

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4561021
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561023
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561023DC
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561024
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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7.5-percent-mini-protean-tgx-gel-10-well-50-ul-with-dual-color-standards-456-1024dc-thumb.jpg
4561024DC
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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456-1093_tn.jpg
4561025
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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7.5-percent-mini-protean-tgx-gel-12-well-20-ul-with-dual-dolor-standards-456-1025dc-thumb.jpg
4561025DC
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561026
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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7.5-percent-mini-protean-tgx-gel-15-well-15-ul-with-dual-color-standards-456-1026dc-thumb.jpg
4561026DC
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561031
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561033
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561033DC
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561034
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561034DC
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561035
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561036
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561036DC
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561041
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561043
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561043DC
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561044
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561044DC
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561045
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561045DC
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561046
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561046DC
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561081
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561083
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561083DC
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561084
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561084DC
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561085
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4-15-percent-mini-protean-tgx-gel-12-well-20-ul-with-dual-color-standards-456-1085dc-thumb.jpg
4561085DC
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561086
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561086DC
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561091
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561093
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561093DC
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561094
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561095
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561096
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561096DC
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4561101
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561103
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561104
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561105
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4561106
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4569031
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4569033
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4569033DC
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4569034
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4569034DC
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4569035
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4569035DC
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards
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4569036
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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any-kd-mini-protean-tgx-gel-15-well-15-ul-with-dual-color-standards-456-9036dc-thumb.jpg
4569036DC
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein™ Dual Color Standards

Accessories

Accessories

Kits & Reagents

Kits & Reagents

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1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
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1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications
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1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
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1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications
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1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610480
Kit for silver staining polyacrylamide gels with the large Dodeca Stainer (1653400), for 12 large gels

Documents

Documents

Number Description Options
10026447
Mini-PROTEAN® TGX™ Precast Gel Quick Start Guide, Rev C
5871
Mini-PROTEAN® TGX™ Precast Gels Product Information Sheet, Rev F
[ Add to Cart (Free) ]
6040
Electrophoresis Guide, Interactive PDF, Rev C
5932
Ready Gel to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev E
5934
NuPAGE Bis-Tris Precast Gels (MOPS Buffer) to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev G
5910
Mini-PROTEAN TGX Precast Gel: A Gel for SDS-PAGE with Improved Stability — Comparison with Standard Laemmli Gels, Rev B
5911
Mini-PROTEAN TGX Precast Gel: A Versatile and Robust Laemmli-Like Precast Gel for SDS-PAGE, Rev A.
6148
Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B
6147
Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B
6507
TGX and TGX Stain-Free Gels: Publications List