iQ-Check Vibrio PCR Detection Kit

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iQ-Check Vibrio PCR Detection Kit

Overview

Validation

Bio-Rad's solutions are validated by international certification bodies against standard methods, so the results you get are results you can trust.

 

AOAC

NF VALIDATION

NordVal

Health Canada
iQ-Check Real-Time PCR Methods
Campylobacter spp. Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
E.coli O157:H7 Open in new window Open in new window
Listeria monocytogenes Open in new window Open in new window Open in new window Open in new window
Listeria spp. Open in new window Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window Open in new window
Salmonella Enteritidis Open in new window
Salmonella Typhimurium Open in new window
STEC 7 major O-groups Open in new window
STEC virulence genes Open in new window
Vibrio spp. Open in new window
RAPID'Chromogenic Methods
Bacillus cereus Open in new window
Campylobacter spp. Open in new window
Coliforms Open in new window Open in new window Open in new window Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
Escherichia coli Open in new window Open in new window Open in new window Open in new window
E. coli O157:H7 Open in new window Open in new window
Listeria monocytogenes

Detection
Enumeration
AL


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Open in new window
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Listeria spp. Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window
Staphylococcus aureus Open in new window

* Alternative analytical methods for agrifood — http://nf-validation.afnor.org/en

Complete method to reliably detect Vibrio species: Vibrio parahaemolyticus, Vibrio cholera, and Vibrio vulnificus.


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  • Description
  • Specifications
  • Ordering
  • Accessories
  • Equipment
  • Documents

Description

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The iQ-Check Vibrio PCR Detection Kit is a multiplex test based on gene amplification and detection by real-time PCR.

This test allows the qualitative detection of V. cholerae, V. parahaemolyticus, and V. vulnificus in food samples previously enriched by culture. The main targeted matrices are seafood, including fish and shellfish (mollusks and crustaceans).

Highly Specific and Reliable

The iQ-Check Vibrio Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result (negative result validation). The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA in the sample.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Vibrio Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Multiple iQ-Check Assays can be run in parallel on the same plate.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Related Products


Specifications

Organism Vibrio species: V. cholerae, V. parahaemolyticus, and V. vulnificus

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iQ-Check Vibrio PCR Detection Kit

12006574
Pkg of 1, kit includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls. For a fast and accurate method to reliably detect the three main Vibrio species: Vibrio parahaemolyticus, Vibrio cholera, and Vibrio vulnificus

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iQ-Check Purification Reagent

12012383
Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests

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$103.00 
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Vibrio Enrichment Broth

12007405
Pkg of 1, 500 g dehydrated selective medium used for enrichment of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus in food products

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TCBS (Thiosulfate Citrate Bile Saccharose)/Agar

3555933
100 ml x 6 bottles

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$91.00 
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Accessories

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list price. If the problem persists, please call customer service at or send an
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Strip Cap Tool for PCR Plates and PCR Tubes

ECT2000
Pkg of 1, tool for seating 8- and 12-cap strips on PCR plates or tubes

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$46.00 
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iQ-Check Deep Well Microplates

3594900
Pkg of 50, 96-well plates plates, deep well

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$285.00 
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Microseal 'B' PCR Plate Sealing Film, adhesive, optical

MSB1001XTU
Pkg of 100, optically clear seal for PCR plates for real-time PCR

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Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

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$298.00 
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X-Pierce Films

3593977
Pkg of 100, PCR plate sealing film, pre-pierced

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$189.00 
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Pre-Pierced Plate Sealing Film

3600040
Pkg of 100, PCR plate sealing film, pre-pierced

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$201.00 
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1.5 ml Conical Tubes, screwcap, sterile

2240110XTU
Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps

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$157.00 
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PCR Tube Strips without Caps, low-profile, 0.2 ml, 8-tube strips

TLS0851XTU
Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)

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$130.00 
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PCR Tube 8-Cap Strips, optical, flat, 8 caps

TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)

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$43.00 
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Multiplate™ 48-Well PCR Plates, low-profile, unskirted, white

MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples

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$163.00 
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Multiplate 96-Well PCR Plates, low-profile, unskirted

MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

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$141.00 
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Equipment

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CFX96 Touch Deep Well Real-Time PCR Detection System

3600037
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

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iQ-Check Prep System

3594911
Automated system for DNA extraction and real-time PCR plate set-up, 1 unit

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Heating Thermoshaker

3594995
Pkg of 1, temperature-controlled shaker for cell lysis, for use with iQ-Check PCR Detection Kits

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Deep Well Block for Thermoshaker

3594996
Pkg of 1, deep well block for thermoshaker

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CFX Manager Software, Industrial Diagnostic Edition (IDE)

3593893
1 unit

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$3,178.00 
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Documents

Number Description Options
7097 Vibrio Flier, Ver A Click to download
10000095779 iQ-Check Vibrio User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
7414 Performance Summary, iQ-Check Vibrio Method Click to download
7094 iQ-Check® Vibrio Quick Guide Click to download
6960 iQ-Check Prep Solution Brochure Click to download
10000058391 iQ-Check Free DNA Removal Solution Product Insert Click to download
7322 iQ-Check Free DNA Removal Solution — Quick Guide Click to download
6834 iQ-Check Free DNA Removal Solution Brochure Click to download
10000119734 iQ-Check Purification Reagent Technical sheet Click to download
FSD_16439 iQ-Check Real-Time PCR Solution Brochure Click to download
P1-226 Poster: Alternative Method for Detection of Vibrio Click to download
7274 Complete Solutions for Service Laboratories Click to download

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