iQ-Check Vibrio PCR Detection Kit

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Overview

Complete method to reliably detect Vibrio species: Vibrio parahaemolyticus, Vibrio cholera, and Vibrio vulnificus.

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Description

Description

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The iQ-Check Vibrio PCR Detection Kit is a multiplex test based on gene amplification and detection by real-time PCR.

This test allows the qualitative detection of V. cholerae, V. parahaemolyticus, and V. vulnificus in food samples previously enriched by culture. The main targeted matrices are seafood, including fish and shellfish (mollusks and crustaceans).

Highly Specific and Reliable

The iQ-Check Vibrio Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result (negative result validation). The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA in the sample.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Vibrio Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Multiple iQ-Check Assays can be run in parallel on the same plate.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Related Products

Specifications

Specifications

Organism
Vibrio species: V. cholerae, V. parahaemolyticus, and V. vulnificus

Ordering

Ordering

items

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12006574
Pkg of 1, kit includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls. For a fast and accurate method to reliably detect the three main Vibrio species: Vibrio parahaemolyticus, Vibrio cholera, and Vibrio vulnificus
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12007405
Pkg of 1, 500 g dehydrated selective medium used for enrichment of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus in food products

Accessories

Accessories

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HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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TLS0851XTU

Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)

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TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
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MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

Equipment

Equipment

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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

Documents

Documents

Number Description Options
7097
Vibrio Flier, Ver A
10000095779
iQ-Check Vibrio User Guide (English, French, German, Italian, Portuguese, Spanish)
7414
Performance Summary, iQ-Check Vibrio Method
7094
iQ-Check® Vibrio Quick Guide
3240
iQ-Check PCR Kits Easy I and II Extraction TUBE Protocol Quick Guide
6960
iQ-Check Prep Solution Brochure
10000058391
iQ-Check Free DNA Removal Solution Product Insert
7322
iQ-Check Free DNA Removal Solution — Quick Guide
6834
iQ-Check Free DNA Removal Solution Brochure
10000119734
iQ-Check Purification Reagent Technical sheet
FSD_16439
iQ-Check Real-Time PCR Solution Brochure
3311
CFX Opus 96 Real-Time PCR System brochure
3309
CFX Opus 96 and CFX Opus Deepwell Real-Time PCR system product information sheet
P1-226
Poster: Alternative Method for Detection of Vibrio
7274
Complete Solutions for Service Laboratories
3276
CFX Manager Software (IDE) iQ-Check Prep sample setup Protocol, Quick Guide