iQ-Check Vibrio PCR Detection Kit

Complete method to reliably detect Vibrio species: Vibrio parahaemolyticus, Vibrio cholera, and Vibrio vulnificus.

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Validation

Ordering Information

Overview

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The iQ-Check Vibrio PCR Detection Kit is a multiplex test based on gene amplification and detection by real-time PCR.

This test allows the qualitative detection of V. cholerae, V. parahaemolyticus, and V. vulnificus in food samples previously enriched by culture. The main targeted matrices are seafood, including fish and shellfish (mollusks and crustaceans).

Highly Specific and Reliable

The iQ-Check Vibrio Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result (negative result validation). The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA in the sample.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Vibrio Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Multiple iQ-Check Assays can be run in parallel on the same plate.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

Lysis reagent for DNA extraction
Fluorescent probes
PCR amplification mix
Positive and negative PCR controls

Specifications

Organism

Vibrio species: V. cholerae, V. parahaemolyticus, and V. vulnificus

Ordering

items

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(Discontinued) iQ-Check Vibrio PCR Detection Kit

12006574

Pkg of 1, kit includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls. For a fast and accurate method to reliably detect the three main Vibrio species: Vibrio parahaemolyticus, Vibrio cholera, and Vibrio vulnificus

(Discontinued) iQ-Check Purification Reagent

12012383

Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests

(Discontinued) Vibrio Enrichment Broth

12007405

Pkg of 1, 500 g dehydrated selective medium used for enrichment of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus in food products

Accessories

(Discontinued) Strip Cap Tool for PCR Plates and PCR Tubes

ECT2000

Pkg of 1, tool for seating 8- and 12-cap strips on PCR plates or tubes

(Discontinued) iQ-Check Deep Well Microplates

3594900

Pkg of 50, 96-well plates plates, deep well

(Discontinued) Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655XTU

Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

(Discontinued) X-Pierce Films

3593977

Pkg of 100, PCR plate sealing film, pre-pierced

(Discontinued) Pre-Pierced Plate Sealing Film

3600040

Pkg of 100, PCR plate sealing film, pre-pierced

(Discontinued) PCR Tube 8-Cap Strips, optical, flat, 8 caps

TCS0803XTU

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)

(Discontinued) Multiplate™ 48-Well PCR Plates, low-profile, unskirted, white

MLL4851XTU

Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples

(Discontinued) Multiplate 96-Well PCR Plates, low-profile, unskirted

MLL9651XTU

Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

(Discontinued) Microseal 'B' PCR Plate Sealing Film, adhesive, optical

17010701

Pkg of 100, optically clear seal for PCR plates for real-time PCR

(Discontinued) 1.5 ml Conical Tubes, screwcap, sterile

17010678

Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps

(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style D), 20–200 µl

17010688

Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style H), 100–1,000 µl

2112036XTU

Pkg of 1,000, 100–1,000 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNas

(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style E), 100–1,000 µl

2112021XTU

Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase, For food application only

(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style B), 2–20 µl

2112006XTU

Pkg of 960, 2–20 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

Equipment

(Discontinued) CFX96 Touch Deep Well Real-Time PCR Detection System

3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.