ELISA Substrate Kits

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ELISA Substrate Kits

Overview

Comparison of ELISA Substrate Systems
  AP HRP
 
ELISA Type pNPP ABTS TMB
Qualitative +++ ++++ ++++
Stop reagent Sodium hydroxide Oxalic acid Sulfuric acid
Color Yellow Blue-green Yellow
Detection wavelength 405 nm 415 nm 450 nm
Quantitative (Endpoint) ++++ +++ +++
Stop reagent Sodium hydroxide Oxalic acid Sulfuric acid
Color Yellow Blue-green Yellow
Detection wavelength 405 nm 415 nm 450 nm
Quantitative (Kinetic) ++ +++ ++++
Color Yellow Blue-green Blue
Detection wavelength 405 nm 415 nm 655 nm
Substrate performance: ++++ excellent; +++, good; ++, fair.
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Bio-Rad offers ELISA substrate kits suitable for use in all microtitration enzyme immunoassays. The Bio-Rad AP substrate kit contains easy-to-use p-nitrophenyl phosphate tablets and dilution buffer concentrate to prepare 500 ml of working solution. Benefits of the AP system include an extremely linear reaction rate and low background values for accurate quantitation by ELISA.

Two convenient substrate systems are available for detection of HRP-labeled antibodies. Both the ABTS (2,2'-azino-di(3-ethylbenzthiazoline)-6-sulfonic acid) peroxidase substrate kit and the TMB (3,3',5,5'-tetramethylbenzidine) peroxidase substrate kit contain liquid reagents that are mixed just prior to use. No weighing or dissolving of hazardous chemicals is necessary. The single-component TMB peroxidase EIA substrate kit makes microplate assays convenient and fast.

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AP Substrate Kit

1721063
Alkaline phosphatase substrates, for immunodetection, includes 100 pNPP tablets, 100 ml 5x concentrate diethanolamine buffer, for 500 ml

0 1721063 /templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1063_0921120752129.xml 1721063 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US jbza3k8m 2017-11-14 07:08:53 true true internet/product_SKU jbza3k8m templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1063_0921120752129.xml LSR 2019-05-04 21:50:41 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556043343093 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 21:50:41 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1063_0921120752129.xml templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1063_0921120752129.xml 1721063-ap-substrate-kit 1721063 AP Substrate Kit AP Substrate Kit Alkaline phosphatase substrates, for immunodetection, includes 100 pNPP tablets, 100 ml 5x concentrate diethanolamine buffer, for 500 ml Alkaline phosphatase substrates, for immunodetection, includes 100 pNPP tablets, 100 ml 5x concentrate diethanolamine buffer, for 500 ml Alkaline phosphatase substrates, for immunodetection, includes 100 pNPP tablets, 100 ml 5x concentrate diethanolamine buffer, for 500 ml /webroot/web/images/lsr/products/microplate_systems/sku_thumb/global/172-1063_tn.jpg /webroot/web/images/lsr/products/microplate_systems/sku_view/global/172-1063_view.jpg /webroot/web/images/lsr/products/microplate_systems/sku_feature/global/172-1063_feature.jpg RepEx 172-1063 Alkaline phosphatase substrates, for immunodetection, includes 100 pNPP tablets, 100 ml 5x concentrate diethanolamine buffer, for 500 ml 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Microplate-Systems/Microplate-Immunoassay-Reagent N 0 1721063, ap, substrate, pnpp, alkaline, phosphatase, elisa, immunoassays, imuunodetection, detection false $254.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721063&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721063&CartQuote=QT&quantity= 1179 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721063&AddToDefaultWishList=true&quantity= 41116100 254.00 1721063
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HRP Substrate Kit

1721064
Horseradish peroxidase substrates, for immunodetection, includes 180 ml ABTS, 20 ml hydrogen peroxide, detection wavelength 415 nm

1 1721064 /templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1064_0921120752171.xml 1721064 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US jbza3kcl 2017-11-14 07:08:53 true true internet/product_SKU jbza3kcl templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1064_0921120752171.xml LSR 2019-05-04 21:50:37 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556043343144 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 21:50:37 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1064_0921120752171.xml templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1064_0921120752171.xml 1721064-hrp-substrate-kit 1721064 HRP Substrate Kit HRP Substrate Kit Horseradish peroxidase substrates, for immunodetection, includes 180 ml ABTS, 20 ml hydrogen peroxide, detection wavelength 415 nm Horseradish peroxidase substrates, for immunodetection, includes 180 ml ABTS, 20 ml hydrogen peroxide, detection wavelength 415 nm Horseradish peroxidase substrates, for immunodetection, includes 180 ml ABTS, 20 ml hydrogen peroxide, detection wavelength 415 nm /webroot/web/images/lsr/products/microplate_systems/sku_thumb/global/172-1064_tn.jpg /webroot/web/images/lsr/products/microplate_systems/sku_view/global/172-1064_view.jpg /webroot/web/images/lsr/products/microplate_systems/sku_feature/global/172-1064_feature.jpg RepEx 172-1064 Horseradish peroxidase substrates, for immunodetection, includes 180 ml ABTS, 20 ml hydrogen peroxide, detection wavelength 415 nm 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Microplate-Systems/Microplate-Immunoassay-Reagent N 0 1721064, hrp, substrate, horseradish, peroxidase, abts, elisa, immunoassays, immunodetection, detection false $191.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721064&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721064&CartQuote=QT&quantity= 1179 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721064&AddToDefaultWishList=true&quantity= 41116100 191.00 1721064
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TMB Peroxidase EIA Substrate Kit

1721066
Peroxidase substrates, for immunodetection, kit contains 180 ml TMB solution and 20 ml hydrogen peroxide, detection wavelength 450 nm

2 1721066 /templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1066_0921120752150.xml 1721066 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US jbza3k5e 2017-11-14 07:08:53 true true internet/product_SKU jbza3k5e templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1066_0921120752150.xml LSR 2019-05-04 21:50:29 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556043343195 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 21:50:29 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1066_0921120752150.xml templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1066_0921120752150.xml 1721066-tmb-peroxidase-eia-substrate-kit 1721066 TMB Peroxidase EIA Substrate Kit TMB Peroxidase EIA Substrate Kit Peroxidase substrates, for immunodetection, kit contains 180 ml TMB solution and 20 ml hydrogen peroxide, detection wavelength 450 nm Peroxidase substrates, for immunodetection, kit contains 180 ml TMB solution and 20 ml hydrogen peroxide, detection wavelength 450 nm Peroxidase substrates, for immunodetection, kit contains 180 ml TMB solution and 20 ml hydrogen peroxide, detection wavelength 450 nm /webroot/web/images/lsr/products/microplate_systems/sku_thumb/global/172-1066_tn.jpg /webroot/web/images/lsr/products/microplate_systems/sku_view/global/172-1066_view.jpg /webroot/web/images/lsr/products/microplate_systems/sku_feature/global/172-1066_feature.jpg RepEx 172-1066 Peroxidase substrates, for immunodetection, kit contains 180 ml TMB solution and 20 ml hydrogen peroxide, detection wavelength 450 nm 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Microplate-Systems/Microplate-Immunoassay-Reagent N 0 1721066, tmb, peroxidase, eia, substrate, elisa, immunoassay, immunoassays, immunodetection, detection, horseradish, hrp false $197.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721066&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721066&CartQuote=QT&quantity= 1179 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721066&AddToDefaultWishList=true&quantity= 41116100 197.00 1721066
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TMB Peroxidase EIA Substrate Kit

1721067
For detection of HRP-labeled antibodies, kit contains 900 ml TMB solution and 100 ml hydrogen peroxide, detection wavelength 450 nm

3 1721067 /templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1067_0921120752089.xml 1721067 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US jbza3ka2 2017-11-14 07:08:53 true true internet/product_SKU jbza3ka2 templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1067_0921120752089.xml LSR 2019-05-04 21:50:29 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556043343253 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 21:50:29 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1067_0921120752089.xml templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1067_0921120752089.xml 1721067-tmb-peroxidase-eia-substrate-kit 1721067 TMB Peroxidase EIA Substrate Kit TMB Peroxidase EIA Substrate Kit For detection of HRP-labeled antibodies, kit contains 900 ml TMB solution and 100 ml hydrogen peroxide, detection wavelength 450 nm For detection of HRP-labeled antibodies, kit contains 900 ml TMB solution and 100 ml hydrogen peroxide, detection wavelength 450 nm For detection of HRP-labeled antibodies, kit contains 900 ml TMB solution and 100 ml hydrogen peroxide, detection wavelength 450 nm /webroot/web/images/lsr/products/microplate_systems/sku_thumb/global/172-1067_tn.jpg /webroot/web/images/lsr/products/microplate_systems/sku_view/global/172-1067_view.jpg /webroot/web/images/lsr/products/microplate_systems/sku_feature/global/172-1067_feature.jpg RepEx 172-1067 For detection of HRP-labeled antibodies, kit contains 900 ml TMB solution and 100 ml hydrogen peroxide, detection wavelength 450 nm 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Microplate-Systems/Microplate-Immunoassay-Reagent N 0 1721067, tmb, peroxidase, eia, substrate, elisa, immunoassay, immunoassays, immunodetection, detection, horseradish, hrp false $530.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721067&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721067&CartQuote=QT&quantity= 1179 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721067&AddToDefaultWishList=true&quantity= 41116100 530.00 1721067
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Single-Component TMB Peroxidase EIA Substrate Kit, 100 ml

1721068
100 ml, single-component EIA substrate solution, contains TMB and H2O2, detection wavelength 450 nm

4 1721068 /templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1068_0921120752071.xml 1721068 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US jbza3k8g 2017-11-14 07:08:53 true true internet/product_SKU jbza3k8g templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1068_0921120752071.xml LSR 2019-05-04 21:50:41 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556909733079 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 21:50:41 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1068_0921120752071.xml templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1068_0921120752071.xml 1721068-single-component-tmb-peroxidase-eia-substrate-kit-100-ml 1721068 Single-Component TMB Peroxidase EIA Substrate Kit Single-Component TMB Peroxidase EIA Substrate Kit, 100 ml 100 ml, single-component EIA substrate solution, contains TMB and H<sub>2</sub>O<sub>2</sub>, detection wavelength 450 nm Single-component, contains 100 ml TMB solution, detection wavelenth 450 nm /webroot/web/images/lsr/products/microplate_systems/sku_thumb/global/172-1068_tn.jpg /webroot/web/images/lsr/products/microplate_systems/sku_view/global/172-1068_view.jpg /webroot/web/images/lsr/products/microplate_systems/sku_feature/global/172-1068_feature.jpg ,VN RepEx 172-1068 100 ml, single-component EIA substrate solution, contains TMB and H2O2, detection wavelength 450 nm 1721068, single-component, tmb, peroxidase, eia, substrate, single, component, horseradish, hrp, elisa, immunoassay, immunoassays, immunodetection, detection 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Microplate-Systems/Microplate-Immunoassay-Reagent N 0 <p>Use Single-Component TMB Peroxidase EIA Substrate for horseradish peroxidase immunoassays. This substrate develops a deep blue color in the presence of peroxidase-labeled conjugate.</p> <ul> <li>One-bottle stabilized chromogenic substrate with long term-stability and lower sensitivity</li> <li>Contains both 3,3&rsquo;,5,5&rsquo; tetramethylbenzidine (TMB) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)</li> <li>Detection wavelength 450 nm</li> <li>Does not contain DMF or DMSO</li> <li>Not suitable for use with assays requiring a precipitating substrate</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 300px;" border="0"> <tbody> <tr> <td style="padding-left:40px" align="left" valign="top"><strong>Volume<br /></strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left:40px" align="left" valign="top">&nbsp; 100 ml<br /></td> <td align="center" valign="top"><span style="font-size: 11px;">1721068</span></td> </tr> <tr> <td style="padding-left:40px" align="left" valign="top">&nbsp; 1 L<br /></td> <td align="center" valign="top"><a href="/en-us/sku/1721072-single-component-tmb-peroxidase-elisa-substrate-kit-1-l"><span style="font-size: 11px;">1721072</span></a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li>Disposable Polystyrene Cuvettes: Standard (<a href="/en-us/sku/2239950-standard-disposable-polystyrene-cuvettes">2239950</a>) or Semimicrovolume (<a href="/en-us/sku/2239955-semimicrovolume-disposable-polystyrene-cuvettes">2239955</a>)</li> <li>Costar 96&ndash;Well Flat&ndash;Bottom EIA Plate (<a href="/en-us/sku/2240096-costar-96-well-flat-bottom-eia-plate">2240096</a>)</li> <li><a href="/en-us/product/microplate-readers/imark-microplate-absorbance-reader">iMark&trade; Microplate Absorbance Reader</a></li> <li><a href="/en-us/product/elisa-substrate-kits">ELISA Substrate Kits</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-assay-kits-cuvettes">Protein Assay Kits</a></li> <li><a href="/en-us/category/microplate-readers">Microplate Readers</a></li> <li><a href="/en-us/category/protein-sample-preparation">Protein Sample Preparation</a></li> <li><a href="/en-us/category/products/chromatography">Chromatography</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> <li><a href="/en-us/category/bio-plex-multiplex-immunoassay-system?ID=85824182-db2a-4c6e-ac82-4e07dd9ab904">Bio-Plex<sup>&reg;</sup> Multiplex Immunoassay System</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Single Component TMB Peroxidase EIA Substrate Kit <a href="/webroot/web/pdf/lsr/literature/Bulletin_9418.pdf" target="_blank">Instruction Manual</a></li> <li>TMB Peroxidase EIA Substrate Kit <a href="/webroot/web/pdf/lsr/literature/Lit-176c.pdf">Instruction Manual</a><a href="/webroot/web/pdf/lsr/literature/Bulletin_1069.pdf"></a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/protein-purification-isolation">Protein Purification and Isolation</a></li> <li><a href="/en-us/applications-technologies/multiplex-immunoassays">Multiplex Immunoassays</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 172-1068.html /webroot/web/html/lsr/products/SKUs/autopopulated/172-1068.html false $88.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721068&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721068&CartQuote=QT&quantity= 1179 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721068&AddToDefaultWishList=true&quantity= 41116100 88.00 1721068
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Single-Component TMB Peroxidase ELISA Substrate Kit, 1 L

1721072
1 L, single-component EIA substrate solution, contains TMB and H2O2, detection wavelength 450 nm

5 1721072 /templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1072_0921120752108.xml 1721072 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US jebrbid4 2018-02-28 05:10:56 true true internet/product_SKU jebrbid4 templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1072_0921120752108.xml LSR 2019-05-04 21:50:30 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556909733123 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 21:50:30 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1072_0921120752108.xml templatedata/internet/product_SKU/data/LSR/Microplate-Systems/Microplate-Immunoassay-Reagent/172-1072_0921120752108.xml 1721072-single-component-tmb-peroxidase-elisa-substrate-kit-1-l 1721072 Single-Component TMB Peroxidase ELISA Substrate Kit Single-Component TMB Peroxidase ELISA Substrate Kit, 1 L 1 L, single-component EIA substrate solution, contains TMB and H<sub>2</sub>O<sub>2</sub>, detection wavelength 450 nm Single-component, contains 1 L TMB solution, detection wavelenth 450 nm /webroot/web/images/lsr/products/microplate_systems/sku_thumb/global/172-1072_tn.jpg /webroot/web/images/lsr/products/microplate_systems/sku_thumb/global/172-1072_tn.jpg /webroot/web/images/lsr/products/microplate_systems/sku_view/global/172-1072_view.jpg ,VN Rick Abruzzo 172-1072 1 L, single-component EIA substrate solution, contains TMB and H2O2, detection wavelength 450 nm 1721072, single-component, tmb, peroxidase, eia, substrate, single, component, horseradish, hrp, elisa, immunoassay, immunoassays, immunodetection, detection 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Microplate-Systems/Microplate-Immunoassay-Reagent N 0 <div id="pane_box_homepage" style="margin-left: 5px; width: 635px;"> <div class="leftcolumn_homepage" style="width: 20px; margin-top: 10px;"><img src="/evportal/framework/skins/evolution/images/info_icon.png" alt="" /></div> <div class="rightcolumn_homepage" style="margin: 10px 0px; width: 604px;"> <p class="infoMsgfontWt">There is no longer a minimum order quantity for this item. Please allow 5&ndash;8 weeks for delivery.</p> </div> <div class="clear" style="height: 1px;">&nbsp;</div> </div> <p>Use Single-Component TMB Peroxidase EIA Substrate for horseradish peroxidase immunoassays. This substrate develops a deep blue color in the presence of peroxidase-labeled conjugate.</p> <ul> <li>One-bottle stabilized chromogenic substrate with long term-stability and lower sensitivity</li> <li>Contains both 3,3&rsquo;,5,5&rsquo; tetramethylbenzidine (TMB) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)</li> <li>Detection wavelength 450 nm</li> <li>Does not contain DMF or DMSO</li> <li>Not suitable for use with assays requiring a precipitating substrate</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 300px;" border="0"> <tbody> <tr> <td style="padding-left:40px" align="left" valign="top"><strong>Volume<br /></strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left:40px" align="left" valign="top">&nbsp; 100 ml<br /></td> <td align="center" valign="top"><a href="/en-us/sku/1721068-single-component-tmb-peroxidase-eia-substrate-kit-100-ml"><span style="font-size: 11px;">1721068</span></a></td> </tr> <tr> <td style="padding-left:40px" align="left" valign="top">&nbsp; 1 L<br /></td> <td align="center" valign="top"><span style="font-size: 11px;">1721072</span></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li>Disposable Polystyrene Cuvettes: Standard (<a href="/en-us/sku/2239950-standard-disposable-polystyrene-cuvettes">2239950</a>) or Semimicrovolume (<a href="/en-us/sku/2239955-semimicrovolume-disposable-polystyrene-cuvettes">2239955</a>)</li> <li>Costar 96&ndash;Well Flat&ndash;Bottom EIA Plate (<a href="/en-us/sku/2240096-costar-96-well-flat-bottom-eia-plate">2240096</a>)</li> <li><a href="/en-us/product/microplate-readers/imark-microplate-absorbance-reader">iMark&trade; Microplate Absorbance Reader</a></li> <li><a href="/en-us/product/elisa-substrate-kits">ELISA Substrate Kits</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-assay-kits-cuvettes">Protein Assay Kits</a></li> <li><a href="/en-us/category/microplate-readers">Microplate Readers</a></li> <li><a href="/en-us/category/protein-sample-preparation">Protein Sample Preparation</a></li> <li><a href="/en-us/category/products/chromatography">Chromatography</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> <li><a href="/en-us/category/bio-plex-multiplex-immunoassay-system?ID=85824182-db2a-4c6e-ac82-4e07dd9ab904">Bio-Plex<sup>&reg;</sup> Multiplex Immunoassay System</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Single Component TMB Peroxidase EIA Substrate Kit <a href="/webroot/web/pdf/lsr/literature/Bulletin_9418.pdf" target="_blank">Instruction Manual</a></li> <li>TMB Peroxidase EIA Substrate Kit <a href="/webroot/web/pdf/lsr/literature/Lit-176c.pdf">Instruction Manual</a><a href="/webroot/web/pdf/lsr/literature/Bulletin_1069.pdf"></a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/protein-purification-isolation">Protein Purification and Isolation</a></li> <li><a href="/en-us/applications-technologies/multiplex-immunoassays">Multiplex Immunoassays</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 172-1072.html /webroot/web/html/lsr/products/SKUs/autopopulated/172-1072.html false $780.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721072&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721072&CartQuote=QT&quantity= 1179 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721072&AddToDefaultWishList=true&quantity= 12000000 780.00 1721072
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Number Description Options
LIT172 Instruction Manual, Alkaline Phosphatase Substrate Kit Click to download
LIT445 Instruction Manual, Peroxidase Substrate Kit, Rev D Click to download
LIT572 Instruction Manual, Single Component TMB Peroxidase EIA Substrate Kit Click to download
LIT452 Instruction Manual, Rabbit Anti-Goat IgG-Horseradish Peroxidase Conjugate, Rev C Click to download

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