ELISA Substrate Kits

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Overview

The Bio-Rad AP substrate kit contains easy-to-use p-nitrophenyl phosphate tablets and dilution buffer concentrate to prepare 500 ml of working solution.
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Description

Description

Bio-Rad offers ELISA substrate kits suitable for use in all microtitration enzyme immunoassays. The Bio-Rad AP substrate kit contains easy-to-use p-nitrophenyl phosphate tablets and dilution buffer concentrate to prepare 500 ml of working solution. Benefits of the AP system include an extremely linear reaction rate and low background values for accurate quantitation by ELISA.

Two convenient substrate systems are available for detection of HRP-labeled antibodies. Both the ABTS (2,2'-azino-di(3-ethylbenzthiazoline)-6-sulfonic acid) peroxidase substrate kit and the TMB (3,3',5,5'-tetramethylbenzidine) peroxidase substrate kit contain liquid reagents that are mixed just prior to use. No weighing or dissolving of hazardous chemicals is necessary. The single-component TMB peroxidase EIA substrate kit makes microplate assays convenient and fast.

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(Discontinued) AP Substrate Kit
1721063
Alkaline phosphatase substrates, for immunodetection, includes 100 pNPP tablets, 100 ml 5x concentrate diethanolamine buffer, for 500 ml
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(Discontinued) HRP Substrate Kit
1721064
Horseradish peroxidase substrates, for immunodetection, includes 180 ml ABTS, 20 ml hydrogen peroxide, detection wavelength 415 nm
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1721066
Peroxidase substrates, for immunodetection, kit contains 180 ml TMB solution and 20 ml hydrogen peroxide, detection wavelength 450 nm
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1721067
For detection of HRP-labeled antibodies, kit contains 900 ml TMB solution and 100 ml hydrogen peroxide, detection wavelength 450 nm
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1721068
100 ml, single-component EIA substrate solution, contains TMB and H2O2, detection wavelength 450 nm
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1721072
1 L, single-component EIA substrate solution, contains TMB and H2O2, detection wavelength 450 nm

Documents

Documents

Number Description Options
LIT172
Instruction Manual, Alkaline Phosphatase Substrate Kit
LIT445
Instruction Manual, Peroxidase Substrate Kit, Rev D
LIT572
Instruction Manual, Single Component TMB Peroxidase EIA Substrate Kit
LIT452
Instruction Manual, Rabbit Anti-Goat IgG-Horseradish Peroxidase Conjugate, Rev C