Overview
The BSE scrapie discriminatory kit for strain typing of transmissible spongiform encephalopathies (TSEs) in small ruminants has been developed to discriminate BSE from scrapie and to confirm atypical scrapie strains.
Discrimination between BSE and scrapie is based on the partial resistance of BSE-infected samples to proteinase K treatment. Comparison between confirmation assay results and discriminatory assay results is possible since the protocol of the discriminatory assay has been directly adapted from the TeSeE Western Blot Assay (Catalog #3551169).
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Blot
Pkg of 10, 0.2 µm, 10 x 15 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting
Pkg of 50, 7.5 x 10 cm, absorbent filter paper, for use with Mini Trans-Blot cassette
Pkg of 5, spacer plate with 1.5 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber
Pkg of 50, 9.5 x 15.2 cm, absorbent filter paper, for use with the Criterion blotter
Pkg of 1 roll, 0.2 µm, 26 cm x 3.3 m, bulk membrane for high binding (150–160 µg/cm2) immunoblotting
1 L, 10x PBS
100 ml, enzyme immunoassay grade polysorbate surfactant (detergent)
Electrophoresis
Pkg of 5, spacer plate with 1.5 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber
Pkg of 10, 0.2 µm, 10 x 15 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting
Pkg of 50, 7.5 x 10 cm, absorbent filter paper, for use with Mini Trans-Blot cassette
Pkg of 50, 9.5 x 15.2 cm, absorbent filter paper, for use with the Criterion blotter