Aquadien DNA Extraction and Purification Kit

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Overview

The Aquadien DNA Extraction and Purification Kit uses tangential microfiltration technology for the best extraction performance, with optimized protocols for PCR analysis of all water and biofilm samples.

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Description

Description

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The Aquadien DNA Extraction and Purification Kit is used for reliable high-yield bacterial DNA extraction and purification from water samples to enhance sensivity and reproducibility of real-time PCR analysis. The kit must be used prior to real-time PCR assays for optimal results.

Key Benefits

  • Developed for efficient bacterial DNA extraction from all water samples, aerosols, and biofilms
  • Allows enhanced detection of bacteria in water samples, including Pseudomonas aeruginosa, Salmonella spp., and E. coli
  • Designed to work with iQ-Check Legionella Real-Time PCR Kits or other real-time PCR analysis
  • Optimized extraction from 1 L of water per sample
  • Rapid detection and quantitation of pathogenic bacteria (24 hr)
  • Standardized protocol for routine monitoring of drinking water, swimming pools, whirlpools, industrial water distribution systems, and other water sources
  • AFNOR/ISO validated by AFNOR Certification according to the NF T90-471 and ISO TS 12869 standards

Principle

The protocol uses alkaline thermal lysis after tangential microfiltration (cross flow filtration), which reduces clogging of membrane pores during sample concentration.

Additional Information

Use the Aquadien W2 Wash Solution (3578119) for samples that clog the membrane and for samples containing PCR inhibitors.

Ordering Information

The Aquadien DNA Extraction and Purification Kit (3578121) contains the components for processing 96 samples.

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3578121
Pkg of 1 kit, includes lysis reagent, tubes, columns, and elution buffer, for 96 samples
3578119
Pkg of 1, 5 ml bottle of Wash Solution W2, for use with Aquadien Bacterial DNA Extraction and Purification Kit, enough for 48 tests
aquadien-lysis-solution-3578125-thumb.jpg
3578125
Pkg of 1, 100 ml bottle, for use with the CIP10-M Air Sampler, sufficient for 32 air samples
iQ-Check-Free-DNA-Removal-16-0708-thumb.jpg
3594970
Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)
12006823
1 L bottle of Legionella DNA Free water

Documents

Documents

Number Description Options
10000134675
Aquadien+iQ-Check Legionella User Guide (English, French, German, Italian, Portuguese, Spanish)
7451
Aquadien DNA Extraction Short Protocol, Quick Guide
7453
Aquadien DNA Extraction Standard Protocol, Quick Guide
7452
Aquadien DNA Extraction Short Protocol for Dirty-Clogging Samples
7454
Aquadien DNA Extraction Standard Protocol for Dirty-Clogging Samples
10000058391
iQ-Check Free DNA Removal Solution Product Insert
7021
iQ-Check Free DNA Removal Solution for Legionella
16414
Legionella Testing Solutions Brochure, Ver E
FSD_17932
Water Safety Booklet - Guide for Products in Water Testing