AG® 501 Mixed-Bed Resins

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AG<sup>&reg;</sup> 501 Mixed-Bed Resins

Overview

AG® 501-X8 Resin Properties
  AG 501-X8 Resin AG 5010-X8 (D) Resin Bio-Rex MSZ 501(D) Resin
Chemical form H+ & OH- H+ & OH- H+ & OH-
Resin Type Mixed bed Mixed bed Mixed bed
Physical form Light and dark gold beads Blue and gold beads Blue and brown beads
Minimum total capacity 1.5 meq/ml 1.5 meq/ml 1.5 meq/ml
Particle diameter 300–1,180 µm 300–1,180 µm 600±100 µm
Mesh size 20–50 20–50 25–35
Matrix Styrene divinylbenzene Styrene divinylbenzene Styrene divinylbenzene
Analytical Grade Resin Selection Guide
Analytical Grade Resin Selection Guide
Stability of Selected Analytical Grade Resins to Various Treatments
Resin
Heat*
Solvents (Alcohols,
Hydrocarbons, etc.)
Oxidation
Reduction
AG 1 OH- form, fair to 50°C
Cl- and other forms,
good to 150°C
Very good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good**
AG 2 OH- form, fair to 30°C
Cl- form, good to 150°C
Very good Slowly dissolves in
hot 15% HNO3
Good**
AG 50W Good to 150°C Very good Slowly dissolves in
hot 15% HNO3
Very good
AG 4-X4 Good to 60°C Good Good Unknown
AG 501-X8 Good to 50°C Very good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good**
AG 11 A8 Good to 100°C Good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good
Bio-Rex 5 Good to 60°C Good Good Excellent
Bio-Rex 70 Good to 100°C Good Good Good
Bio-Rex MSZ 501(D) Good to 50°C Very good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good**
Chelex 100 Good to 75°C Good Breaks down in strong
oxidizing agents
Unknown
* All anion resins may be autoclaved except the CH- form; all cation exchange resins may be autoclaved.
** AG 1, AG 2, AG 501, and Bio-Rex MSZ 501 (D) resins will slowly break down in the presence of sulfur-containing reducing agents.
AG Resin Equivalent Diameters
Analytical Grade Resin Wet Mesh and Equivalent Diameters
Wet mesh (U.S. Standards) 16 20 40 50 80 100 140 200 270 325 400
Diameter, µm 1,180 850 425 300 180 150 106 75 53 45 38
Guidelines for Water Deionization
Column Size
(diameter x length)
Weight of Resin
(g)
Approximate Volume
of Deionized Water*
(liters)
0.5 x 20 2.6 1.5
1.0 x 50 27.0 6.7
1.5 x 100 118.0 29.0
2.5 x 100 325.0 80.0
5.0 x 100 1,300.0 325.0
15.0 x 120 13.6 3,500.0
* Based on using 100 ppm NaCl. This volume will vary depending on initial water quality.
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Mixed bed resins are used for deionizing water or other non-ionic substances such as urea, acrylamide, formamide, or glyoxal. Deionization is the complete removal of all ionic species from a solution.

AG 501-X8 and Bio-Rex MSZ 501(D) resins are mixed-bed resins containing equivalent amounts of AG 50W-X8 strong cation exchange resin (H+ form) and AG 1-X8 strong anion exchange resin (OH- form). Molecular biology grade AG 501-X8 resin is certified to contain no endo- or exonucleases and no ligase inhibitors while biotechnology grade AG 501-X8 resin is certified to contain less than 100 microorganisms per gram.

AG 501- X8(D) resin and Bio-Rex MSZ 501(D) resin have a blue dye irreversibly bound to the anion exchange resin, which turns from blue to gold when the exchange capacity is exhausted.

Applications: AG 501-X8 and Bio-Rex MSZ 501(D) mixed bed resins may be used to prepare non-ionic reagents for critical analytical applications. Either the batch or column method may be used to obtain purified urea, acrylamide, formamide, glyoxal, or PEG, although the batch technique is much more common.

Larger volumes and special packaging for industrial applications are available on request.

Number Description Options
LIT205 Instruction Manual, AG 501-X8 and Bio-Rex MSZ 501(D) Mixed Bed Resin, Rev B Click to download
6904 Speciality Beads: AG® Resins Flier, Ver A Click to download

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

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