Affi-Gel and Affi-Prep Protein A Resins

Chromatography on Affi-Gel and Affi-Prep protein A resins yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species.

Advantages include:

• High purity of IgGs
• High affinity for mammalian IgG
• High capacities for mouse IgG₁ as well as other subclasses with MAPS optimized buffer

In addition, Affi-Prep resins offer high linear flow rates up to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar), and high chemical stability, which allows sanitization with 0.1 M NaOH.

With Affi-Gel and Affi-Prep protein A resins and MAPS II buffers, you can purify up to 10 mg of IgG₁/ml of gel. This is 8–10 times higher than with standard methods. In addition, the MAPS process and Affi-Gel protein A permit greater binding of mouse IgG₁ than do immobilized protein G resin.