Affi-Gel Protein A Resin

Chromatography on Affi-Gel protein A media yields highly purified immunoglobulins (IgGs), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species.

Features and Benefits

• High purity of IgGs
• High affinity for mammalian IgG
• High capacities for mouse IgG₁ as well as other subclasses with MAPS optimized buffer

With Affi-Gel Protein A Media and MAPS II buffers, you can purify up to 10 mg of IgG₁/ml of gel. This is 8–10 times higher than is possible with standard methods. In addition, the MAPS process and Affi-Gel protein A media permit greater binding of mouse IgG₁ than do immobilized protein G media.