Affi-Gel and Affi-Prep Protein A Resins

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Overview

Chromatography on Affi-Gel and Affi-Prep Protein A Resins yield highly purified immunoglobulins (IgG), selectively removes IgG from serum, and adsorbs immune complexes for antigen affinity purification.

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Description

Description

Chromatography on Affi-Gel and Affi-Prep protein A resins yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species.

Advantages include:

  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer

In addition, Affi-Prep resins offer high linear flow rates up to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar), and high chemical stability, which allows sanitization with 0.1 M NaOH.

With Affi-Gel and Affi-Prep protein A resins and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than with standard methods. In addition, the MAPS process and Affi-Gel protein A permit greater binding of mouse IgG1 than do immobilized protein G resin.

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1536153
5 ml, ready-to-use protein A crosslinked agarose support
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1536154
50 ml, ready-to-use protein A crosslinked agarose support
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1536159
Ready-to-use affinity media kit, includes 5 ml Affi-Gel protein A support, Affi-Gel Protein A MAPS II buffers, 1 x 10 cm Econo-Column column; for 500 mg of mouse IgG1
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1560006
5 ml, ready-to-use pressure-stable polymer support
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1560005
25 ml, ready-to-use pressure-stable polymer support
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1536161
Optimized buffer concentrate, for use with Affi-Gel and Affi-Prep protein A media, makes 5 L
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12009236
Pkg of 5, 1 ml, prepacked affinity columns, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system
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12009237
Pkg of 1, 5 ml, prepacked affinity column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

Documents

Documents

Number Description Options
LIT153
Instruction Manual, Econo-Pac® Protein A Kit, Protein A Columns, Rev B
LIT88
Instruction Manual, Affi-Gel® Protein A MAPS II Kit
LIT79
Instruction Manual, Affi-Prep Protein A MAPS II Kit
LIT230
Instruction Manual, Affi-Prep Protein A Matrix
LIT379
Affi-Gel Protein A Agarose for IgG Purification