Affinity Chromatography Resins


FEATURED PRODUCT | Nuvia™ IMAC Resin
Nuvia IMAC Resin
  • For purification of histidine-tagged recombinant proteins
  • High-capacity
  • Pressure-stable polymer

Overview

Bio-Rad offers four types of affinity chromatography resin: Nuvia and Profinity for affinity-tagged protein purification; Affi-Gel for laboratory-scale affinity purification; Affi-Prep for process-scale affinity purification; and UNOsphere SUPrA for monoclonal antibody purification.


Category Products

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    Nuvia IMAC Resin
    Nuvia IMAC (immobilized metal affinity chromatography) Resin provides an ultra-high capacity matrix over a wide range of flow rates and pH. Get reproducible separation of biomolecules free of contaminants.
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    Profinity™ Epoxide Resin
    Profinity Epoxide Resin is an activated affinity chromatography support for the immobilization of biomolecules that contain nucleophiles such as amino, thiol, or hydroxyl groups.
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    Profinity IMAC Resin
    Profinity IMAC (immobilized metal affinity chromatography) Resin is an affinity chromatography support for protein purification of recombinant histidine-tagged products.
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    Affi-Gel and Affi-Prep Protein A Resins

    Chromatography on Affi-Gel and Affi-Prep Protein A Resins yield highly purified immunoglobulins (IgG), selectively removes IgG from serum, and adsorbs immune complexes for antigen affinity purification.

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    Affi-Gel® Blue Gel
    Affi-Gel Blue Gel is a crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye. The blue dye functions as an ionic, hydrophobic, aromatic, or sterically active binding site.
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    DEAE Affi-Ge Blue Gel
    DEAE Affi-Gel Blue Gel is a bifunctional matrix for both affinity purification and ion exchange chromatography prepared by coupling Cibacron Blue F3GA and diethylaminoethyl groups to Bio-Gel A.
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    CM Affi-Gel® Blue Gel
    CM Affi-Gel Blue Gel contains Cibacron Blue F3GA dye covalently coupled to CM Bio-Gel A. This bifunctional affinity chromatography gel binds both albumin and serum proteases.
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    Affi-Gel® Heparin Gel
    Affi-Gel heparin affinity chromatography gel is useful in protein purification for a range of products such as coagulation factors, plasma proteins, polynucleotide polymerases, nucleases, lipases, lipoproteins, and proteases.
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    Affi-Prep® Polymyxin Support
    Affi-Prep polymyxin affinity chromatography support consists of 2–4 mg of USP-grade polymyxin per ml of the macroporous polymeric Affi-Prep support. It binds endotoxins from a number of strains of gram-negative bacteria.
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    Affi-Gel® Boronate Gel
    Affi-Gel boronate-derivatized polyacrylamide gel has a high binding capacity and is ideal for the affinity purification of molecules with adjacent coplanar cis-hydroxyl groups (cis-diols).
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    Affi-Gel® Hz Hydrazide Gel
    Affi-Gel Hz Hydrazide Gel activated affinity chromatography support couples immunoglobulin G (IgG) molecules via carbohydrate moieties on the Fc region of antibody molecules.
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    Affi-Gel® 102 Gel

    Affi-Gel 102 affinity chromatography support is for use with 1-ethyl-3–(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) coupling reagent, which immobilizes ligands that contain primary or terminal carboxyl groups.

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    UNOsphere SUPrA Resin

    UNOsphere SUPrA affinity chromatography resins are designed for process-scale purification of monoclonal antibodies. These affinity resins are built on the proven UNOsphere matrix for predictable performance.