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iMark™ Microplate Absorbance Reader

iMark™ Microplate Absorbance Reader

The iMark microplate absorbance reader offers fast reading speeds, extensive onboard software capabilities, a preprogrammed validation protocol (for the Checkmark reader performance check plate).

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Overview

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The iMark™ Microplate Absorbance Reader, with a wavelength range of 400–750 nm, is an economical, high-performance solution for a wide range of applications, including immunoassays with colorimetric substrates, such as ELISA, and protein assays such as Bradford and Lowry. The instrument can be used as a stand-alone reader or be controlled by a PC or Mac with Microplate Manager® 6 Software

Features and Benefits of the iMark Reader

  • Fast microplate reading speeds
  • Extensive onboard assay configuration
  • Powerful data analysis capabilities
  • Convenient built-in shaker
  • Space-saving, wide-format internal thermal graphical printer
  • Simplified standalone operation
  • Compact, easy-to-use, and lightweight design
  • Compatible with different types of 96-well microplates (flat, U-, V-bottom) and 8- or 12-well strip plates; maximum plate height: 16 mm

Features and Benefits of the iMark Reader Onboard Software

  • Wide applicability for life science and clinical laboratories
  • Programmable kinetic reading
  • Security login that allows locking and unlocking of key protocol settings by the administrator
  • Generous memory capacity for protocol creation and storage
  • Comprehensive curve-fit selections
  • Extensive reporting functions
  • Preprogrammed protocols for the Checkmark™ Reader performance verification kit, to validate the IQ/OQ/PQ of the iMark Reader in compliance with GLP
  • Other life science and specialized protocols included such as for the Bio-Rad TSE kits and a growing number of food testing and clinical kits

The iMark Reader comes with six standard absorbance filters: 415, 450, 490, 595, 655, and 750 nm. Bio-Rad also supplies custom filters at 5 nm intervals from 400 to 750 nm on request.

Plate Compatibility

  • Compatible with standard 96-well microplates 128 mm L x 88 mm W
  • Plate height cannot exceed 16 mm
  • Clear flat-bottom plates  are recommended such as Bio-Rad's Costar 96–Well Flat–Bottom EIA Plate (2240096)
  • U-Bottom plates can be used, but V-bottom plates are not recommended

Specifications

Wavelength range
400–750 nm
Photometric range
0.0–3.5 OD
Linearity
≤1.0% from 0.0–2.0 OD; ≤2.0% from 0.0–3.0 OD
Accuracy
≤1.0% or 0.010 from 0.000–3.000 OD at 490 nm
Precision
1.0% or 0.005 OD from 0.0–2.0 OD; 1.5% from 2.0–3.0 OD
Resolution
0.001 OD
Filter wheel capacity
8
Plate shaking (3 speeds)
Low, mid, high
Duration, sec
0–999
Read time
6 sec at single wavelength, 10 sec at dual wavelengths
Data output
Onboard graphical thermal printer and USB2 interface with PC or Mac data stations
Data storage
Calender/clock functions; 64 assay protocols
Multilanguage support
4 languages, LCD indication supported; printout report supported
Dimensions (W x D x H), cm (in)
34.6 x 37.7 x 16.4 (13.6 x 14.8 x 6.5)
Weight, kg (lb)
5.5 (12)

Ordering

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1707009
100/240 V, microplate washer, includes bottle and tubing, 8-port manifold, aerosol protection cover, compatible with flat-, U-, and V-bottom plates

Accessories

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1682006
Pkg of 1, replacement lamp for use with iMark microplate absorbance reader
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1682007
Pkg of 3 rolls, replacement thermal paper for the built-in printer on the iMark microplate absorbance reader (#168-1130)
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1689520
Version 6.3, software intended for collection, analysis, and output of absorbance data from Bio-Rad's iMark™ microplate absorbance reader and xMark™ microplate absorbance spectrophotometer only
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1686940
Absorbance calibration plate for use with all Bio-Rad absorbance microplate readers, includes Checkmark software to allow GLP compliance

Kits & Reagents

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5000122

Colorimetric assay kit, includes RC Reagents Package, DC™ Protein Assay Reagents Package, BSA standard; 450 standard assays

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5000121

Colorimetric assay kit, includes RC Reagents Package, DC™ Protein Assay Reagents Package, bovine γ-globulin standard; 450 standard assays

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5000204

Colorimetric assay kit, includes 1x dye reagent (1 L), bovine γ-globulin standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml)

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5000202

Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml)

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5000201

Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard (5 x 2 mg/ml); 200 standard assays or 4,000 microplate assays

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5000112
Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, BSA standard; 450 assays
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5000111
Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, bovine γ-globulin standard; 450 assays
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5000002

Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine serum albumin standard; for 440 standard assays or 2,200 microplate assays

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5000001

Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine γ-globulin standard; for 440 standard assays or 2,200 microplate assays

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1722055
Panel includes 10 ml each rabbit anti-mouse subclass-specific antiserum to mouse IgG-1, IgG-2a, IgG-2b, IgG-3, IgM, IgA, κ- and λ-chains, sufficient for 100 typings
1722051
Antibody isotyping kit for ELISA, sufficient for 100 typings (800 tests), includes mouse isotyping panel (#172-2055), affinity purified goat anti-rabbit IgG (H + L)
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1721068

100 ml, single-component EIA substrate solution, contains TMB and H2O2, detection wavelength 450 nm

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1721067
For detection of HRP-labeled antibodies, kit contains 900 ml TMB solution and 100 ml hydrogen peroxide, detection wavelength 450 nm
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1721066
Peroxidase substrates, for immunodetection, kit contains 180 ml TMB solution and 20 ml hydrogen peroxide, detection wavelength 450 nm
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1721063
Alkaline phosphatase substrates, for immunodetection, includes 100 pNPP tablets, 100 ml 5x concentrate diethanolamine buffer, for 500 ml
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1721037
1 ml, EIA-grade, enzyme-antibody conjugate
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1721034
1 ml, EIA-grade, enzyme-antibody conjugate
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1721019
1 ml, EIA-grade, enzyme-antibody conjugate
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1721017
1 ml, EIA-grade, enzyme-antibody conjugate
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1721011
2 ml, EIA-grade, enzyme-antibody conjugate

Filters

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1681010
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681011
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
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1681012
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681013
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681014
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681015
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681016
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681017
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681018
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681019
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681020
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681021
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681022
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681023
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681024
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681026
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681029
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681030
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681031
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681032
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681033
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681034
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681035
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681036
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681037
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681038
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681039
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681040
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681041
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681042
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681043
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681044
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681045
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681046
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681047
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681049
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681050
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681051
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681052
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681053
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681054
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
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1681055
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681056
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
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1681057
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
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1681058
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
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1681060
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681061
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681062
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681063
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681064
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681065
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681066
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681067
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681068
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681069
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681070
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681071
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681072
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681073
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681074
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681075
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681076
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681077
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681078
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681079
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
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1681080
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers

Microplates

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2240096

Box of 4 packages, 25 high binding enzyme immunoassay plates per package (100 plates), polystyrene

Documents

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