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Vericheck ddPCR Empty-Full Capsid AAV9 Kit

Vericheck ddPCR Empty-Full Capsid AAV9 Kit #17010082

With the absolute quantification of Droplet Digital™ PCR, the Vericheck ddPCR Empty-Full Capsid Kit requires only small sample volume of crude lysate or purified samples to simultaneously measure capsid and viral titer for AAV serotype 9.

Available Safety Data Sheets

Overview

The Vericheck ddPCR Empty-Full Capsid AAV9 Kit enables a quick and simple measurement of the empty-full capsid ratio that is needed to produce AAV vectors. The empty-full capsid ratio is a Critical Quality Attribute (CQA) in AAV vector production, as high amounts of empty capsids may cause unwanted immunotoxicity. This kit provides consistent and reliable capsid content measurements across the AAV production process with high precision, multi-sample type compatibility, low-sample input, fast turnaround time, and easy data analysis.

Features & Benefits

  • Sample type compatibility — Crude lysate and purified
  • Low sample input — Only 106 virus particles required to get results
  • Fast turnaround — Up to 14 samples per plate in less than 1 day
  • High precision — Coefficient of variation (CV) less than 5%
  • Simplified data analysis – Streamlined process providing simultaneous results for genome titer, capsid titer, and empty-full ratio

Product Contents

The Vericheck ddPCR Empty-Full Capsid AAV9 Kit (17010082) contains the following products:

Catalog NumberDescriptionQuantity
12022102Vericheck ddPCR Empty Full Capsid Reagent Box1
1601291020x ddPCR ITR2 Assay1
1601291120x ddPCR Capsid Detection Assay1
12022016Ligase1
1202207410x Ligase Buffer1
12022017Dilution Buffer1
12022018Stop Solution1
1601287210x PLA Splint1
10042051Dnase1
1202202010x Dnase Buffer1
12007991ddPCR Supermix for Probes No dUTP 0.7ML2
Catalog NumberDescriptionQuantity
12022098Vericheck ddPCR Empty Full Capsid Kit AAV9 Box1
12022088AAV9 Antibody 11
12022075AAV9 Antibody 21
12022369AAV9 Positive Control Labelled1

Instrument Compatibility

InstrumentCatalog Number
QX ONE Droplet Digital PCR System12006536
QX200 AutoDG Droplet Digital PCR System consisting of:
QX200 Droplet Reader
Automated Droplet Generator
1864100
1864003
1864101
QX600 Droplet Digital PCR Reader12013328
QX Manager Software, Regulatory Edition12012172
QX ONE Software, Regulatory Edition12012078
QX Manager, Premium Edition12018108

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