Overview
The Vericheck ddPCR Empty-Full Capsid Kit provides enables a quick and simple check of the empty-full capsid ratio that is needed for the production of produce AAV vectors. The Empty full capsid ratio is a CQA in AAV vector production, as since high amounts of empty capsids may cause unwanted immunotoxicity. This kit provides consistent and reliable capsid content measurements across the AAV production process with high precision, multi-sample type compatibility, low low-sample input, fast turnaround time, and easy data analysis
Features & Benefits
- Sample Type Compatibility — crude lysate and purified sample.
- Low Sample Input — only 106 virus particles required to get results
- Fast Turnaround — up to 14 samples per plate in less than 1 day
- High Precision — CVs closer to 2%
- Data Analysis — simplified workflow with results for genome titer, capsid titer, and empty full ratio
Product Contents
| Catalog Number | Description | Quantity |
| 12022102 | Vericheck ddPCR Empty Full Capsid Reagent Box | 1 |
| 16012910 | 20x ddPCR ITR2 Assay | 1 |
| 16012911 | 20x ddPCR Capsid Detection Assay | 1 |
| 12022016 | Ligase | 1 |
| 12022074 | 10x Ligase Buffer | 1 |
| 12022017 | Dilution Buffer | 1 |
| 12022018 | Stop Solution | 1 |
| 16012872 | 10x PLA Splint | 1 |
| 10042051 | Dnase | 1 |
| 12022020 | 10x Dnase Buffer | 1 |
| 12007991 | ddPCR Supermix for Probes No dUTP 0.7ML | 2 |
| Catalog Number | Description | Quantity |
| 12022035 | Vericheck ddPCR Empty Full Capsid Kit AAV8 Box | 1 |
| 12022037 | AAV8 Antibody 1 | 1 |
| 12022044 | AAV2 Antibody 2 | 1 |
| 12022377 | AAV8 Positive Control | 1 |
Instrument Compatibility
| Instrument | Catalog Number |
| QX ONE Droplet Digital PCR System | 12006536 |
| QX200 AutoDG Droplet Digital PCR System consisting of: QX200 Droplet Reader Automated Droplet Generator | 1864100 1864003 1864101 |
| QX600 Droplet Digital PCR Reader | 12013328 |
| QX Manager Software, Regulatory Edition | 12012172 |
| QX ONE Software, Regulatory Edition | 12012078 |
| QX Manager, Premium Edition | 12018108 |