Bio-Plex Pro Mouse Th17 Assays

Bio-Plex Pro Mouse Th17 Assays

Overview

Magnetic bead–based assays for the detection and measurement of 17 mouse Th17 biomarkers. Available in 3 premixed assay panels, 17 singleplex sets, or as custom x-Plex and Express assays.

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Description

Description

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The Bio-Plex Pro mouse Th17 magnetic bead–based immunoassays detect and measure the levels of 17 biomarkers for Th17 cells  in diverse matrices such as serum, plasma, and tissue culture supernatants. IL-17 producing T helper cells (Th17 cells) are a new subset of T helper cells recently found to be associated with autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, and lupus. The markers in these assays were selected for their direct relevance to the mouse Th17 pathway.

Based on 6.5 µm magnetic beads, these assays are compatible with magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as all-in-one, space-saving complete kits
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target

Flexible Ordering Options

  • Premixed panels — complete ready-to-use kits: Panel A (6-plex), or TGF-β (3-plex)
  • Singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection bodies; requires a reagent kit (12002798) for magnetic separation and a vial of standards (171I50001 for group I cytokines, 171IA0001 for Th17 cytokines, or 171X40001 for TGF-β assays) for a complete assay
  • Custom premixed assays — available as Bio-Plex x-Plex assays built using the Bio-Plex Assay Builder
  • Custom assays (mixed at the bench) —  available as Bio-Plex Express assays built using the Bio-Plex Assay Builder

Instrument Compatibility

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Ordering

Ordering

items

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12010828
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, control, reagents, and diluents for detecting 10 mouse cytokines all in one kit
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M6000007NY
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 6 mouse cytokines all in one kit
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171W4001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, and sealing tape for the detection of TGF-β1, TGF-β2, TGF-β3 all in one kit

Accessories

Accessories

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171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays
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171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
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30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles

Rgnts & Stds

Rgnts & Stds

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12002798
1 x 96-well, includes mouse HP detection antibody diluent, standard diluent, sample diluent, assay buffer, wash buffer, streptavidin-PE, flat bottom plate, and sealing tape for use with magnetic separation methods
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12005847
10 x 96-well, includes mouse HP detection antibody diluent, standard diluent, sample diluent, assay buffer, wash buffer, streptavidin-PE, flat bottom plate, and sealing tape for use with magnetic separation methods
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171I50001

Pkg of 1 vial, lyophilized cytokine standard for detecting 23 mouse cytokines, enough for 96 assays; for use with Mouse Cytokine Group I Assays (IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-17A, Eotaxin, G-CSF, GM-CSF, IFN-γ, KC, MCP-1 (MCAF), MIP-1α, MIP-1β, RANTES, TNF-α) and Reagent Kit V(12002798)

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171I10501

Pkg of 50 lot-matched vials, lyophilized cytokine standard for detecting 23 mouse cytokines, enough for 50 x 96 assays; for use with Mouse Cytokine Group I Assays ((IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-17A, Eotaxin, G-CSF, GM-CSF, IFN-γ, KC, MCP-1 (MCAF), MIP-1α, MIP-1β, RANTES, TNF-α) and Reagent Kit V(12002798)

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171IA0001

Pkg of 1 vial, lyophilized standards for detecting 11 mouse cytokines, enough for 96 assays; for use with Mouse Th17 Cytokine Assays and Reagent Kit V (12002798)

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171IA0501
Pkg of 50, lot-matched vials, lyophilized standards for detecting 11 mouse cytokines, enough for 96 assays; for use with Mouse Th17 Cytokine Assays and Reagent Kit V (12002798)
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171X40001
Pkg of 1 vial, lyophilized TGF-β standard for detecting TGF-β1, TGF-β2, and TGF-β3 analytes, enough for 96 assays; for use with TGF-β Assays and Reagent Kit III (171304090M)
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171X40501
Pkg of 50 lot-matched vials, lyophilized TGF-β standard for detecting TGF-β1, TGF-β2, and TGF-β3 analytes, enough for 50 x 96 assays; for use with TGF-β Assays and Reagent Kit III (171304090M)

Singleplexes

Singleplexes

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171G5002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IL-1β. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Cytokine Standards Group I (171I50001), which are not included.
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171G5007M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IL-6. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Cytokine Standards Group I (171I50001), which are not included.
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171G5009M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IL-10. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Cytokine Standards Group I (171I50001), which are not included.
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171G5013M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IL-17A. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Cytokine Standards Group I (171I50001), which are not included.
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171G5017M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse IFN-γ. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Cytokine Standards Group I (171I50001), which are not included.
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171G5023M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting mouse TNF-α. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Cytokine Standards Group I (171I50001), which are not included.
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171GA006M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting mouse IL-25. Requires Reagent Kit V (12002798) for magnetic separation, and 1 vial of Mouse Th17 Cytokine Standards (171IA0001), which are not included.

Documents

Documents

Number Description Options
6118
Analysis of Murine Th17 Cytokine Profiles using Bio-Plex Pro Mouse Th17 Panel
[ Add to Cart (Free) ]
6335
Bio-Plex™ Pro Multiplex Immunoassays Analyte Guide
5405
Bio-Plex® Multiplex System Brochure, Ver E
[ Add to Cart (Free) ]
6110
Bio-Plex Pro Mouse Th17 Assays Product Information Sheet
10000118395
Bio-Plex Pro Mouse Th17 Cytokine Quick Guide
2993
Bio-Plex Glossary, Rev A
3100
Bio-Plex Cytokine Assay Comparison, Rev E
5297
Bio-Plex Pro™ Assay Reference Guide, Ver D
3235
Comparison of Bio-Plex Pro Assay Performance Using the Bio-Plex 200 and Luminex xMAP INTELLIFLEX Systems