Aurum™ Total RNA 96 Kit
The Aurum total RNA 96 kit isolates high-quality DNA-free total RNA from cultured cells, bacteria, and yeast in a 96-well format for high-throughput total RNA isolation.
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Overview
The Aurum total RNA 96 kit isolates high-quality DNA-free total RNA from cultured cells, bacteria, and yeast in a 96-well format for high-throughput total RNA isolation. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a 96-well plate format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.
Features include:
- High-throughput total RNA isolation in less than 60 minutes
- RNase-free reagents and plasticware, including DNase I for removal of genomic DNA at no additional cost
- Rapid silica binding-based purification technology in a 96-well plate format
- Easy-to-use spin- or vacuum-compatible protocol
Specifications
Format
96-well plate
Method
Spin- or vacuum-mediated silica binding
Number of preps
192 (2 x 96-well plate)
Maximum starting material amounts
1 x 106 cultured cells
8 x 108 bacterial cells
2 x 107 yeast cells
8 x 108 bacterial cells
2 x 107 yeast cells
Binding capacity
>40 µg purified total RNA
Purity
A260/A280 of 1.9–2.1
RNA size distribution
>200 nucleotides
Preparation time
<60 min (including DNase I digest)
Maximum elution volume
80 µl
Minimum elution volume
30 µl*
10 µg/1 x 106 NIH 3T3 cells
17 µg/1 x 109 HeLa cells
11 µg/2 x 107 S. cerevisiae cells
10 µg/1 x 106 NIH 3T3 cells
17 µg/1 x 109 HeLa cells
11 µg/2 x 107 S. cerevisiae cells
Yield**
5 µg/8 x 108 E. Coli cells
5 µg/8 x 108 B. cereus cells
5 µg/8 x 108 B. cereus cells
*The elution volume is reduced to 30µl when
isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).
isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).
**Yields will vary depending on the
developmental stage, tissue type or cell line,
and growth conditions used.
developmental stage, tissue type or cell line,
and growth conditions used.
Ordering
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(Discontinued) Aurum™ Total RNA 96 Kit
7326800
DNA-free RNA isolation kit, includes RNA binding plates, collection microplates, lyophilized DNase I, RNase-free reagents, sealing tape, for 2 x 96-well preps
Accessories
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(Discontinued) DNase I
7326828
Pkg of 1 vial, refill reagent for Aurum total RNA kits, RNase-free, lyophilized
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(Discontinued) Aurum DNase Dilution Solution
7326805
20 ml, refill reagent for Aurum total RNA kits
Image
(Discontinued) Aurum Total RNA Wash Low-Stringency Solution
7326804
60 ml, refill reagent for Aurum total RNA kits
Image
(Discontinued) Aurum Total RNA Wash High-Stringency Solution
7326803
150 ml, refill reagent for Aurum total RNA kits, RNase-free
Image
(Discontinued) Aurum Total RNA Lysis Solution
7326802
85 ml, refill reagent for Aurum total RNA kits, RNase-free
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(Discontinued) Aurum Total RNA Elution Solution
7326801
20 ml, refill reagent for Aurum total RNA kits, RNase-free
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