Got Protein? Kit

Based on the Bio-Rad Quick Start™ Bradford protein assay, the Got Protein™ Kit is designed to introduce students to proteomics and provides the tools for them to develop their own protein-based experiments. In this laboratory activity, students use absorbance values from a set of protein samples with known concentrations to create a standard curve on linear graph paper. Protein concentrations of their test samples, such as milk, egg, sports drink, or saliva, can then be extrapolated by hand or plotted using a graphing utility such as Microsoft Excel software.

Features and Benefits

• Inquiry-based activity
• Integrates biology, chemistry, and physics in relation to protein structure and function

With this kit, students are able to:

• Measure protein concentrations
• Apply Beer's law
• Learn to use a spectrophotometer
• Learn to use a micropipet

How It Works

The Bradford method is based on the color development formed when the dye Coomassie Blue G–250 binds to protein. There is a correlation between the amount of blue color and the amount of protein in the sample: the more protein, the more intense the blue color. The simplicity of the assay allows the results to be measured qualitatively by eye or quantitatively with a spectrophotometer.

More Information

• One 45 min laboratory activity
• Provides sufficient materials for eight student workstations, up to four students per workstation.

Additional Information

This kit is a resource for the HOSA competitive event in Biotechnology. To learn more, visit HOSA.org or bio-rad.com/HOSA.

This hands-on activity is integrated with the Lt Biology Collection, the online learning platform from ADInstruments.