SEQuoia Complete Stranded RNA Library Prep Kit

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Overview

The SEQuoia Complete Stranded RNA Library Prep Kit is a new RNA-Seq library prep kit with a proprietary enzyme designed to address the sensitivity and workflow limitations of current enzymes.

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Description

Description

Frequently Asked Questions

SEQuoia FAQ

Learn more about how to use the SEQuoia RNA Library Prep Kit.

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The SEQuoia Complete Stranded RNA Library Prep Kit is a high-performance RNA-Seq kit that captures long and short RNAs in a single library, even from limited and low-quality samples. The streamlined workflow minimizes the number of pipetting steps and reduces the overall protocol time to less than 4 hours. The kit allows construction of libraries that are >99% stranded and suitable for next-generation sequencing on Illumina platforms and includes access to SEQSense Analysis Solution and Toolkit, a dedicated secondary data analysis pipeline.

Featuring SEQzyme, a proprietary engineered enzyme that couples cDNA synthesis with adapter addition in a continuous synthesis reaction. The unique enzymatic properties effectively captures all types and sizes of RNA species in a novel enzymatic reaction, significantly improving the diversity and quality of RNA libraries, even from limited or degraded RNA samples.

Experience the Power of SEQzyme

Features and Benefits

Highly Efficient and Streamlined Library Prep Workflow

The unique enzymatic properties of SEQzyme produce robust, high-quality RNA library. The kits are optimized to facilitate efficient library synthesis using a wide range of input RNA (100 pg to 1 μg) and are compatible with automated next-generation sequencing library construction. The streamlined workflow minimizes the number of pipetting steps and greatly reduces the overall protocol time to less than 4 hours.

Diverse Libraries with Low Quality and Limited Input Samples

The kit constructs a stranded (>99%) library that captures all RNA biotypes, both long and short RNAs, even from poor quality RNAs (for example, FFPE tissue samples). The feature enables researchers to discover biologically relevant differences in more areas of the transcriptome.

miRNA, piRNA, sncRNA, tRNA, snRNA, mRNA, lncRNA, lincRNA

Dedicated Data Analysis Toolkit

SEQSense Analysis Solutions and Toolkit, which is available through a web portal, or can be downloaded and run locally, enables users to trim raw sequencing data and align sequences to a reference genome. The output provides specific quality control metrics to assess the quality of the sequencing data as well as a BAM file, which can be used with tertiary analysis packages to perform advanced analyses.

Applications and Uses

  • Differential gene expression and whole transcriptome profiling of high- and low-quality RNA samples
    • Sorted or single cell
    • Micro-dissection
    • Characterization of non-polyadenylated RNAs
    • Gene fusion identification
    • Single nucleotide variation discovery
  • Biomarker discovery
  • Splice junctions identification

Included Components

Each kit comes with 2 reagent boxes. Box A includes reagents for RNA fragmentation, cDNA synthesis, and library preparation. The kit is stored at –20°C:

  • Reagent A Fragmentation Mix
  • Reagent B End Repair Enzyme
  • Reagent C Poly(A) Buffer
  • Reagent D Poly(A) Polymerase
  • Reagent E SEQzyme Mix
  • Reagent F Amplification Mix

Box B includes magnetic bead for purification. The kit is stored at 4°C

SEQuoia Dual Indexed Primers are available and can be ordered separately.

 

Configuration Options

Catalog No. Description Size
17005726 SEQuoia Complete Stranded RNA Library Prep Kit 24 reactions
17005710 SEQuoia Complete Stranded RNA Library Prep Kit 96 reactions
12011928 SEQuoia Dual Indexed Primers Set, 12 vials of Unique Dual Indexes 96 reactions
12011930 SEQuoia Dual Indexed Primers Plate, one 96-well plate of Unique Dual Indexes 96 reactions


 

Specifications

Specifications

Description
Whole-transcriptome sequencing
Input Range
100 pg–1 µg RNA
Input Types
High- and low-quality RNA samples, including degraded FFPE sample
Transcript Coverage
>99% strandedness
Total Run Time
<4 hours
Variant Class
Gene fusions, novel transcripts, single nucleotide variation, transcript variants
System Compatibility
Compatible with Illumina sequencing platforms
Product Configuration
24 or 96 reaction kit sizes
Shelf life
Guaranteed 6 months from shipment when properly stored

Ordering

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17005726
A high-performance RNA sequencing (RNA-Seq) kit that captures long and short RNAs in a single library, even from limited and low-quality samples. The kit provides all of the components required to make an RNA-Seq library for Illumina platforms. The size of the kit is for 24 reactions.
Image
17005710_th.jpg
17005710
A high-performance RNA sequencing (RNA-Seq) kit that captures long and short RNAs in a single library, even from limited and low-quality samples. The kit provides all of the components required to make an RNA-Seq library for Illumina platforms. The size of the kit is for 96 reactions
Image
12011928_th.jpg
12011928

For use with SEQuoia Complete Stranded RNA Library Prep Kits. Contains 12 unique dual indexed primers supplied in 12 separate vials, for 96 reactions

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12011930_th.jpg
12011930
For use with SEQuoia Complete Stranded RNA Library Prep Kits. Contains 96 unique dual indexed primers plated in a 96-well plate, for 96 reactions

Accessories

Accessories

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TBC0802
Pkg of 20, bag of 12 x 8 thin-wall polypropylene tube strips and 12 x 8-cap strips for PCR, natural (1,920 tubes and 1,920 caps)
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TBC1202
Pkg of 20, bag of 8 x 12 thin-wall polypropylene tube strips and 8 x 12-cap strips for PCR, natural (1,920 tubes and 1,920 caps)

Documents

Documents

Number Description Options
7273
SEQuoia Complete Stranded RNA Library Prep Kit Product Information Sheet
3220
Explore the SEQuoia Workflow for Revolutionized RNA Sequencing Product Information Sheet
10000119323
Instruction Manual, SEQuoia Complete Stranded RNA Library Prep Kit
10000119322
Instruction Manual, SEQuoia Dual Indexed Primers
3268
Capture More of the Transcriptome in Less Time