Science of Opioid Dependence Kit

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Overview

Have your students explore the environmental and genetic links to opioid dependence as they design a human genetic research study.

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Description

Description

Now Available
COVID-19 Hands-On Lesson

Investigate the cause of a real SARS-CoV-2 spreading event using the materials in the Science of Opioid Dependence Kit

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DNA gels

Run your DNA gels in 10 minutes!

Find Out How

The Science of Opioid Dependence Kit puts your students in the roles of doctor and researcher as they explore potential genetic links to opioid dependence. In this kit, your students will outline a human genetic research study; they will conduct gel electrophoresis on pre-amplified PCR patient DNA samples to determine whether a mutation in a human dopamine receptor gene influences opioid dependence. Your students will also consider environmental factors that contribute to dependence as a phenotype.

Features and Benefits

  • Curriculum connections to genetics, neurobiology, DNA structure, PCR, and statistical analysis
  • Pre-amplified PCR DNA samples to teach PCR without a thermal cycler
  • Five activities with optional timelines and branch points
  • Reagents and consumables for 8 lab stations (32 students)

Students will

  • Explore the links between opioid dependence, genetics, the neurobiology of reward pathways, and environment factors
  • Design a human genetic research study and experiments
  • Analyze pre-amplified PCR patient DNA samples by agarose gel electrophoresis
  • Review class and published allele frequency data with optional chi-square analysis

Product Comparison

 
17005316EDU
Science of Opioid
Dependence Kit
(17005316EDU)
170052973EDU_PD_Detail
Science of Opioid Dependence Kit plus Fast Blast Electrophoresis Reagents
(17005297EDU)
17005313EDU_PD_Detail
Science of Opioid Dependence Kit plus UView Electrophoresis Reagents
(17005313EDU)
DNA Samples
 
 
 
PCR Molecular Weight Ruler
 
 
 
Orange G Loading Dye, 5x
 
 
 
1.5 ml Microcentrifuge tubes
 
 
 
Fast Blast DNA Stain, 500x  
 
 
Electrophoresis Buffer, TAE, 50x  
 
 
Certified Molecular Biology Agarose  
 
 
UView 6x Loading Dye and Stain    
 
 

Ordering

Ordering

items

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17005316EDU
Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, loading dye, and microcentrifuge tubes for 32 students; educational use only
17005297EDU_SKU_TN.jpg
17005297EDU
Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, loading dye, Fast Blast DNA stain, agarose, TAE buffer, and microcentrifuge tubes for 32 students; educational use only
17005313EDU_SKU_TN.jpg
17005313EDU
Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, UView 6x Loading Dye and Stain, agarose, TAE buffer, and microcentrifuge tubes for 32 students; educational use only

Accessories

Accessories

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1660531EDU
Compact long-wave transilluminator with auto safety shutoff; education use only
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1664000EDU
Agarose gel electrophoresis cell, includes tank, lid, cables, 7 x 10 cm gel tray, two 8-well combs, does not include casing gates; education use only
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1645050EDU
Power supply for basic applications such as submerged horizontal gel electrophoresis, includes power cord
166-0551EDU_tn.jpg
1660551EDU
Pkg of 1, 2–20 μl, adjustable-volume micropipet, includes digital dial, tip ejector, accommodates standard pipet tips; education use only
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1660506EDU
Pkg of 1, 2–20 μl adjustable-volume micropipet, includes digital dial micrometer, tip ejector, accommodates standard pipet tips, fully autoclavable; education use only
166-0507edu_tn.jpg
1660507EDU
Pkg of 1, 20–200 μl adjustable-volume micropipet, includes digital dial micrometer, tip ejector, accommodates standard pipet tips, fully autoclavable; education use only
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1660512EDU
Pkg of 1, color-coded green micropipet, accommodates standard pipet tips; education use only
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(Discontinued) BR-35 Pipet Tips
2239035EDU
Pkg of 1,000, 2–200 µl standard pipet tip, bulk, autoclavable, yellow; education use only
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(Discontinued) Green Racks
1660481EDU
Pkg of 5, for organizing up to 80 x 1.5/2.0 ml microcentrifuge tubes in each rack; education use only

Refills

Refills

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1660450EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only
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1660462EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, UView 6x Loading Dye and Stain, buffer; education use only
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1665112EDU
1,000 µl, 6x dual-action nontoxic fluorescent nucleic acid stain and loading dye; education use only
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1660742EDU

100 ml, TAE nucleic acid electrophoresis buffer solution, pH 8.0; education use only

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1613015EDU
8-well precast gel, TAE, 1.0% agarose, 7 x 10 cm; education use only
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1613116EDU
5 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases; education use only
161-3100EDU_tn.jpg
1613100EDU
25 g, general purpose agarose for nucleic acid electrophoresis, certified free of inhibitors, DNases, RNases; education use only
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1660742EDU

100 ml, TAE nucleic acid electrophoresis buffer solution, pH 8.0; education use only

223-9480EDU_tn.jpg
2239480EDU
Pkg of 500, graduated micro test tube with pierceable top, 1.5 ml, autoclavable, natural; education use only

Documents

Documents

Number Description Options
10000113752
Science of Opioid Dependence Kit Instructor's Guide
10000113753
Science of Opioid Dependence Kit Student Guide
7176
Opioid Dependence Infographic