Science of Opioid Dependence Kit

Have your students explore the environmental and genetic links to opioid dependence as they design a human genetic research study.

Ordering Information

Overview

The Science of Opioid Dependence Kit puts your students in the roles of doctor and researcher as they explore potential genetic links to opioid dependence. In this kit, your students will outline a human genetic research study; they will conduct gel electrophoresis on pre-amplified PCR patient DNA samples to determine whether a mutation in a human dopamine receptor gene influences opioid dependence. Your students will also consider environmental factors that contribute to dependence as a phenotype.

Features and Benefits

Curriculum connections to genetics, neurobiology, DNA structure, PCR, and statistical analysis
Pre-amplified PCR DNA samples to teach PCR without a thermal cycler
Five activities with optional timelines and branch points
Reagents and consumables for 8 lab stations (32 students)

Students will

Explore the links between opioid dependence, genetics, the neurobiology of reward pathways, and environment factors
Design a human genetic research study and experiments
Analyze pre-amplified PCR patient DNA samples by agarose gel electrophoresis
Review class and published allele frequency data with optional chi-square analysis

Product Comparison

	Science of Opioid Dependence Kit (17005316EDU)	Science of Opioid Dependence Kit plus Fast Blast Electrophoresis Reagents (17005297EDU)	Science of Opioid Dependence Kit plus UView Electrophoresis Reagents (17005313EDU)
DNA Samples
PCR Molecular Weight Ruler
Orange G Loading Dye, 5x
1.5 ml Microcentrifuge tubes
Fast Blast DNA Stain, 500x
Electrophoresis Buffer, TAE, 50x
Certified Molecular Biology Agarose
UView 6x Loading Dye and Stain

Specifications

# Workstations

# Students per workstation

# Lab sessions (50-minute labs)

2-5

Storage temperature (upon delivery)

-20°C as indicated

Kit Contents

DNA sample 1

215 µl

DNA sample 2

250 µl

Molecular weight ruler

200 µl

Orange G loading Dye (5x)

1 ml

Microcentrifuge tubes, 1.5 ml

Instructions

Answer Guide

Instructor and Student Guides available for free download

Ordering

items

Use the filters below to refine results!

(Discontinued) Science of Opioid Dependence Kit

17005316EDU

Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, loading dye, and microcentrifuge tubes for 32 students; educational use only

(Discontinued) Science of Opioid Dependence Kit plus Fast Blast Electrophoresis Reagents

17005297EDU

Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, loading dye, Fast Blast DNA stain, agarose, TAE buffer, and microcentrifuge tubes for 32 students; educational use only

(Discontinued) Science of Opioid Dependence Kit plus UView Electrophoresis Reagents

17005313EDU

Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, UView 6x Loading Dye and Stain, agarose, TAE buffer, and microcentrifuge tubes for 32 students; educational use only

Refills

(Discontinued) Small Fast Blast DNA Electrophoresis Reagents Pack

1660450EDU

Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

(Discontinued) Small UView DNA Electrophoresis Reagents Pack

1660462EDU

Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, UView 6x Loading Dye and Stain, buffer; education use only

(Discontinued) UView™ 6x Loading Dye, 1 ml

1665112EDU

1,000 µl, 6x dual-action nontoxic fluorescent nucleic acid stain and loading dye; education use only

(Discontinued) 50x Tris/Acetic Acid/EDTA (TAE)

1660742EDU

100 ml, TAE nucleic acid electrophoresis buffer solution, pH 8.0; education use only

(Discontinued) 1% TAE Mini ReadyAgarose™ Precast Gel, 7.1 x 10 cm, 8-well

1613015EDU

8-well precast gel, TAE, 1.0% agarose, 7 x 10 cm; education use only

(Discontinued) Certified™ Molecular Biology Agarose

1613116EDU

5 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases; education use only

(Discontinued) Certified Molecular Biology Agarose

1613100EDU

25 g, general purpose agarose for nucleic acid electrophoresis, certified free of inhibitors, DNases, RNases; education use only

(Discontinued) 1.5 ml EZ Micro Test Tubes

2239480EDU

Pkg of 500, graduated micro test tube with pierceable top, 1.5 ml, autoclavable, natural; education use only. Note: This item is now available under the new material number 12021144EDU.

Documents

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