Description
Description
The Science of Opioid Dependence Kit puts your students in the roles of doctor and researcher as they explore potential genetic links to opioid dependence. In this kit, your students will outline a human genetic research study; they will conduct gel electrophoresis on pre-amplified PCR patient DNA samples to determine whether a mutation in a human dopamine receptor gene influences opioid dependence. Your students will also consider environmental factors that contribute to dependence as a phenotype.
Features and Benefits
- Curriculum connections to genetics, neurobiology, DNA structure, PCR, and statistical analysis
- Pre-amplified PCR DNA samples to teach PCR without a thermal cycler
- Five activities with optional timelines and branch points
- Reagents and consumables for 8 lab stations (32 students)
Students will
- Explore the links between opioid dependence, genetics, the neurobiology of reward pathways, and environment factors
- Design a human genetic research study and experiments
- Analyze pre-amplified PCR patient DNA samples by agarose gel electrophoresis
- Review class and published allele frequency data with optional chi-square analysis
Product Comparison
 Science of Opioid Dependence Kit (17005316EDU) |
 Science of Opioid Dependence Kit plus Fast Blast Electrophoresis Reagents (17005297EDU) |
 Science of Opioid Dependence Kit plus UView Electrophoresis Reagents (17005313EDU) |
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| DNA Samples |
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| PCR Molecular Weight Ruler |
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| Orange G Loading Dye, 5x |
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| 1.5 ml Microcentrifuge tubes |
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| Fast Blast DNA Stain, 500x |
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| Electrophoresis Buffer, TAE, 50x |
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| Certified Molecular Biology Agarose |
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| UView 6x Loading Dye and Stain |
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Ordering
Ordering
items
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(Discontinued) Science of Opioid Dependence Kit
17005316EDU
Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, loading dye, and microcentrifuge tubes for 32 students; educational use only
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17005297EDU
Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, loading dye, Fast Blast DNA stain, agarose, TAE buffer, and microcentrifuge tubes for 32 students; educational use only
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17005313EDU
Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, UView 6x Loading Dye and Stain, agarose, TAE buffer, and microcentrifuge tubes for 32 students; educational use only
Accessories
Accessories
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(Discontinued) UView Mini Transilluminator
1660531EDU
Compact long-wave transilluminator with auto safety shutoff; education use only
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(Discontinued) Mini-Sub® Cell GT Cell Tank and Lid
1664000EDU
Agarose gel electrophoresis cell, includes tank, lid, cables, 7 x 10 cm gel tray, two 8-well combs, does not include casing gates; education use only
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(Discontinued) PowerPac Basic Power Supply
1645050EDU
Power supply for basic applications such as submerged horizontal gel electrophoresis, includes power cord
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(Discontinued) Classroom 2–20 μl Digital Micropipet
1660551EDU
Pkg of 1, 2–20 μl, adjustable-volume micropipet, includes digital dial, tip ejector, accommodates standard pipet tips; education use only
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(Discontinued) Professional Adjustable-Volume 2–20 μl Digital Micropipet
1660506EDU
Pkg of 1, 2–20 μl adjustable-volume micropipet, includes digital dial micrometer, tip ejector, accommodates standard pipet tips, fully autoclavable; education use only
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(Discontinued) Professional Adjustable-Volume 20–200 μl Digital Micropipet
1660507EDU
Pkg of 1, 20–200 μl adjustable-volume micropipet, includes digital dial micrometer, tip ejector, accommodates standard pipet tips, fully autoclavable; education use only
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(Discontinued) 10 µl Fixed-Volume Micropipet
1660512EDU
Pkg of 1, color-coded green micropipet, accommodates standard pipet tips; education use only
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(Discontinued) BR-35 Pipet Tips
2239035EDU
Pkg of 1,000, 2–200 µl standard pipet tip, bulk, autoclavable, yellow; education use only
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(Discontinued) Green Racks
1660481EDU
Pkg of 5, for organizing up to 80 x 1.5/2.0 ml microcentrifuge tubes in each rack; education use only
Refills
Refills
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(Discontinued) Small Fast Blast DNA Electrophoresis Reagents Pack
1660450EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only
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(Discontinued) Small UView DNA Electrophoresis Reagents Pack
1660462EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, UView 6x Loading Dye and Stain, buffer; education use only
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(Discontinued) UView™ 6x Loading Dye, 1 ml
1665112EDU
1,000 µl, 6x dual-action nontoxic fluorescent nucleic acid stain and loading dye; education use only
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(Discontinued) 50x Tris/Acetic Acid/EDTA (TAE)
1660742EDU
100 ml, TAE nucleic acid electrophoresis buffer solution, pH 8.0; education use only
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(Discontinued) 1% TAE Mini ReadyAgarose™ Precast Gel, 7.1 x 10 cm, 8-well
1613015EDU
8-well precast gel, TAE, 1.0% agarose, 7 x 10 cm; education use only
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(Discontinued) Certified™ Molecular Biology Agarose
1613116EDU
5 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases; education use only
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(Discontinued) Certified Molecular Biology Agarose
1613100EDU
25 g, general purpose agarose for nucleic acid electrophoresis, certified free of inhibitors, DNases, RNases; education use only
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(Discontinued) 1.5 ml EZ Micro Test Tubes
2239480EDU
Pkg of 500, graduated micro test tube with pierceable top, 1.5 ml, autoclavable, natural; education use only
Documents
Documents
Login Required Number Description Options
10000113752
Science of Opioid Dependence Kit Instructor's Guide
10000113753
Science of Opioid Dependence Kit Student Guide
7176
Opioid Dependence Infographic