Overview
Deliver precise RNA target quantification of up to seven targets using the QX700 One-Step RT-ddPCR Kit for Probes.* This kit can be used with the QX700 ddPCR Series Systems.*
Features and Benefits
- Convenient single-reaction format for absolute quantification by Droplet Digital PCR
- Combined reaction conditions minimize variability, contamination, and sample loss
- Contains all components required for hydrolysis probe–based reverse transcription Droplet Digital PCR (RT-ddPCR), except primers, probe(s), and template
- Can multiplex up to 7 targets
- Optimized enzyme blend enables partitioning of RNA samples into droplets while keeping the enzymes inactive until the reverse transcription reaction is performed at 50°C
- Novel engineered reverse transcriptase allows improved performance with GC-rich targets and secondary structures
- RNase inhibitor protects RNA throughout the entire workflow
- Compatible with all QX700 ddPCR instruments*
- Manufactured under good manufacturing practice (GMP) and International Organization for Standardization (ISO 13485:2016) guidelines to guarantee consistent and reliable high-quality products
Applications and Uses
- Absolute quantification
- Gene expression analysis
- Viral load detection
- Pathogen detection
Packaging Options
| Number of 5 µL Reactions | Ideal For | Key Benefits | Catalog Number |
|---|---|---|---|
| 200 | Smaller scale projects | Minimizes expired product and contamination due to repeated pipetting | 12025316 |
| 500 | Most popular size | Flexible package size | 12025504 |
| 2,500 | Higher throughput applications | Convenient larger volume to improve workflow | 12025531 |
Additional Equipment
This kit requires the use of one of the following instruments:
- QX700 E Droplet Digital PCR System
- QX700 S Droplet Digital PCR System
- QX700 HT Droplet Digital PCR System
Related Products
More Information
The QX700 One-Step RT-ddPCR Kit for Probes* is optimized for use with the QX700 ddPCR Series Systems.*
*For Research Use Only. Not for use in diagnostic procedures.
Specifications
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The QX700 One-Step RT-ddPCR Kit for Probes* contains 200 x 5 µL reactions. It can be used for the absolute quantification of target RNA with the QX700 Droplet Digital™ PCR (ddPCR™) Series Systems.*
The QX700 One-Step RT-ddPCR Kit for Probes* contains 500 x 5 µL reactions. It can be used for the absolute quantification of target RNA with the QX700 Droplet Digital™ PCR (ddPCR™) Series Systems.*
The QX700 One-Step RT-ddPCR Kit for Probes* contains 2,500 x 5 µL reactions. It can be used for the absolute quantification of target RNA with the QX700 Droplet Digital™ PCR (ddPCR™) Series Systems.*
Additional Consumables
The RDG16 Cartridges are designed for partitioning nucleic acid samples and performing digital PCR with the QX700™ E, QX700™ S, and QX700™ HT Droplet Digital™ PCR Systems.*
*For Research Use Only. Not for use in diagnostic procedures.
Downloads
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QX700 System Control Software Configuration FileSOFT-QX700-CONFIG
Add new configuration to Standard and Premium editions of QX700 ddPCR System Control Software v1.4 or lower for use with new QX700 supermixes