Overview
Deliver maximum PCR efficiency, specificity, and sensitivity in Droplet Digital PCR using the QX700 ddPCR EvaGreen® Supermix.* This 5x concentrated, ready-to-use mix can be used with the QX700 ddPCR Series Systems* and supports the detection of double-stranded DNA targets after amplification using commercially available EvaGreen® assays.
Features & Benefits
- Contains all components required for EvaGreen® based Droplet Digital PCR, which increases reliability, consistency, and convenience. Primers and template are not included
- Enables double-stranded DNA detection—only primers are needed to amplify and detect product
- Optimized for use with Bio-Rad’s PrimePCR Assays
- Hot-start polymerase keeps enzyme inactive during sample droplet partitioning
- Multiplex up to 2 targets and save on price per answer while getting more data with less sample and fewer reactions
- Manufactured under good manufacturing practice (GMP) and International Organization for Standardization (ISO 13485:2016) guidelines to guarantee consistent and reliable high-quality products
- Compatible with all QX700 ddPCR instruments*
Applications and Uses
- Absolute quantification
- Gene expression analysis
- Copy number detection
- Multiple target analysis
Packaging Options
| Number of 5 µL Reactions | Supermix volume, mL | Ideal Use | Key Benefits | Catalog Number |
|---|---|---|---|---|
| 200 | 2 x 0.1 mL | Smaller scale projects | Minimizes expired product and contamination due to repeated pipetting | 12025290 |
| 500 | 5 x 0.1 mL | Most popular size | Flexible package size | 12025474 |
| 2,500 | 2 x 1.5 mL | Higher throughput applications | Convenient larger volume to improve workflow | 12025517 |
Additional Equipment
This digital PCR supermix requires the use of one of the following instruments:
- QX700 E Droplet Digital PCR System
- QX700 S Droplet Digital PCR System
- QX700 HT Droplet Digital PCR System
Related Products
More Information
The QX700 ddPCR EvaGreen® Supermix* is optimized for use with the QX700 ddPCR Series Systems.*
*For Research Use Only. Not for use in diagnostic procedures.
Specifications
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The QX700 ddPCR EvaGreen® Supermix* can be used with the QX700 Droplet Digital™ PCR (ddPCR) Series Systems.* It is a 5x concentrated supermix that contains 200 x 5 µL reactions.
*For Research Use Only. Not for use in diagnostic procedures.
The QX700 ddPCR EvaGreen® Supermix* can be used with the QX700 Droplet Digital™ PCR (ddPCR) Series Systems.* It is a 5x concentrated supermix that contains 500 x 5 µL reactions.
*For Research Use Only. Not for use in diagnostic procedures.
The QX700 ddPCR EvaGreen® Supermix* can be used with the QX700 Droplet Digital™ PCR (ddPCR) Series Systems.* It is a 5x concentrated supermix that contains 2500 x 5 µL reactions.
*For Research Use Only. Not for use in diagnostic procedures.
Additional Consumables
The RDG16 Cartridges are designed for partitioning nucleic acid samples and performing digital PCR with the QX700™ E, QX700™ S, and QX700™ HT Droplet Digital™ PCR Systems.*
*For Research Use Only. Not for use in diagnostic procedures.
Downloads
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QX700 System Control Software Configuration FileSOFT-QX700-CONFIG
Add new configuration to Standard and Premium editions of QX700 ddPCR System Control Software v1.4 or lower for use with new QX700 supermixes