QX700™ One-Step RT-ddPCR Kit for Probes #12025504
The QX700 One-Step RT-ddPCR Kit for Probes* contains 500 x 5 µL reactions. It can be used for the absolute quantification of target RNA with the QX700 Droplet Digital™ PCR (ddPCR™) Series Systems.*
*For Research Use Only. Not for use in diagnostic procedures
Safety Data Sheet
Overview
Deliver precise RNA target quantification of up to seven targets using the QX700 One-Step RT-ddPCR Kit for Probes.* This kit can be used with the QX700 ddPCR Series Systems.*
Features and Benefits
- Most popular and flexible package size. Ideal for most applications
- Convenient single-reaction format for absolute quantification by Droplet Digital PCR
- Contains all components required for hydrolysis probe–based RT-ddPCR, except primers, probe(s), and template
- Can multiplex up to 7 targets
- Optimized enzyme blend enables partitioning of RNA samples into droplets while keeping the enzymes inactive until the reverse transcription reaction is performed at 50°C
- Novel engineered reverse transcriptase allows improved performance with GC-rich targets and secondary structures
- RNase inhibitor protects RNA throughout the entire workflow
- Compatible with all QX700 ddPCR instruments*
Applications and Uses
- Absolute quantification
- Gene expression analysis
- Viral load detection
- Pathogen detection
| Number of 5 µL Reactions | Ideal Use | Key Benefits | Catalog Number |
|---|---|---|---|
| 200 | Smaller scale projects | Minimizes expired product and contamination due to repeated pipetting | 12025316 |
| 500 | Most popular size | Flexible package size | 12025504 |
| 2,500 | Higher throughput applications | Convenient larger volume to improve workflow | 12025531 |
Additional Consumables
In addition to the QX700 One-Step RT-ddPCR Kit for Probes,* we offer these consumables for completing your digital PCR workflow:
Equipment
This kit requires the use of one of the following instruments:
- QX700 E Droplet Digital PCR System
- QX700 S Droplet Digital PCR System
- QX700 HT Droplet Digital PCR System
Supporting Documents
- Product Insert
- (Flyer coming soon)
*For Research Use Only. Not for use in diagnostic procedures.
Specifications
Recommended Products
The QX700 ddPCR EvaGreen® Supermix* can be used with the QX700 Droplet Digital™ PCR (ddPCR) Series Systems.* It is a 5x concentrated supermix that contains 500 x 5 µL reactions.
*For Research Use Only. Not for use in diagnostic procedures.
The naica® IQ/OQ Kit is a ready-to-use kit to be used for the operational qualification (OQ) of all of the configurations of the QX700™ E, QX700™ S, and QX700™ HT Droplet Digital™ PCR (ddPCR™) Systems* by qualified laboratory personnel trained to perform digital PCR.
*For Research Use Only. Not for use in diagnostic procedures.