Overview
Deliver maximum PCR efficiency, specificity, and sensitivity in Droplet Digital PCR using the QX700 ddPCR EvaGreen® Supermix.* This 5x concentrated, ready-to-use mix can be used with the QX700 ddPCR Series Systems* and supports the detection of double-stranded DNA targets after amplification using commercially available EvaGreen® assays.
Features and Benefits
- Ideal packaging size for smaller scale projects. Small volume minimizes expired product and contamination due to repeated pipetting
- Contains all components required for EvaGreen® based Droplet Digital PCR, except primers and template
- Enables double-stranded DNA detection—only primers are needed to amplify and detect product
- Optimized for use with Bio-Rad’s PrimePCR Assays
- Hot-start polymerase keeps enzyme inactive during sample droplet partitioning
- Multiplex up to 2 targets
- Compatible with all QX700 ddPCR instruments*
Applications and Uses
- Absolute quantification
- Gene expression analysis
- Copy number detection
- Multiple target analysis
| Number of 5 µL Reactions | Supermix volume, mL | Ideal Use | Key Benefits | Catalog Number |
|---|---|---|---|---|
| 200 | 2 x 0.1 mL | Smaller scale projects | Minimizes expired product and contamination due to repeated pipetting | 12025290 |
| 500 | 5 x 0.1 mL | Most popular size | Flexible package size | 12025474 |
| 2,500 | 2 x 1.25 mL | Higher throughput applications | Convenient larger volume to improve workflow | 12025517 |
Additional Consumables
In addition to the QX700 ddPCR EvaGreen® Supermix,* we offer these consumables for completing your digital PCR workflow:
Equipment
This digital PCR supermix requires the use of one of the following instruments:
- QX700 E Droplet Digital PCR System
- QX700 S Droplet Digital PCR System
- QX700 HT Droplet Digital PCR System
Supporting Documents
- Product Insert
- (Flyer coming soon)
*For Research Use Only. Not for use in diagnostic procedures.
Specifications
Recommended Products
The QX700 One-Step RT-ddPCR Kit for Probes* contains 500 x 5 µL reactions. It can be used for the absolute quantification of target RNA with the QX700 Droplet Digital™ PCR (ddPCR™) Series Systems.*
*For Research Use Only. Not for use in diagnostic procedures
The naica® IQ/OQ Kit is a ready-to-use kit to be used for the operational qualification (OQ) of all of the configurations of the QX700™ E, QX700™ S, and QX700™ HT Droplet Digital™ PCR (ddPCR™) Systems* by qualified laboratory personnel trained to perform digital PCR.
*For Research Use Only. Not for use in diagnostic procedures.