Quantum Prep® Plasmid Kits

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Quantum Prep Plasmid Kits

Overview

Specifications
  Quantum Prep Plasmid
Miniprep Kit
Quantum Prep Plasmid
Midiprep Kit
Application Transfections, fluorescent sequencing, cloning Transfections, fluorescent sequencing, cloning
Format Spin columns Spin columns
Cell Culture Volume 1–10 ml 20–40 ml
DNA Yield Up to 20 µg Up to 300 µg
Preparation Time 15 minutes 45 minutes
# of Preps 100 20
Plasmid Purification Kit Selection Guide
Kit DNA
Yield
Preparation
Time
Bacterial Culture
Density or Volume
Purification
Format
Binding
Matrix
Growth
Media
Aurum plasmid mini >20µg 10 min Up to 12 OD • ml* Vacuum**
and spin
Silica
membrane
Standard
Quantum Prep miniprep >20µg 15 min 1–10 ml Spin Diatomaceous
earth
Enriched
or standard

* (OD600 of undiluted culture) x (culture volume in ml) = #of OD • ml. 1 OD600 is equivalent to approximately 8 x 108 cells/ml.
** Using the Aurum vacuum manifold.

High Purity Plasmid DNA
High Purity Plasmid DNA

QC analysis of Quantum Prep–purified plasmid DNA. Fluorescent DNA sequence analysis using the ABI PRISM Dye Terminator kit and analyzed on an ABI 377A DNA sequencer.

High Plasmid Yield
High Plasmid Yield

High yield of Quantum Prep kits. Yields as high as 24 µg can be obtained from a 1.5 ml culture of DH5αF'[pB1] grown in Terrific Broth.

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The consistent high yields achieved with Quantum Prep plasmid kits are due to the high-affinity binding of DNA to the unique Quantum Prep DNA binding matrix. This patented matrix, a special grade of diatomaceous earth prepared with a proprietary treatment to activate the largest possible surface area, is available only from Bio-Rad. The large surface area allows the use of enriched growth media, such as Terrific Broth, to obtain the highest yields. Quantum Prep kits use an easy spin-based procedure to quickly go from cell culture to purified plasmid DNA. Plasmid DNA is recovered in water or TE for immediate use in all downstream applications.

Features include:

  • High purity and yield of plasmid DNA
  • Easy spin-column procedure to significantly reduce plasmid DNA isolation time
  • Stringent QC specifications ensure high-quality plasmid DNA for immediate use in all downstream molecular biology applications
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Quantum Prep® Plasmid Miniprep Kit

7326100
100 preps, plasmid DNA purification kit, includes Quantum Prep matrix, solutions (cell resuspension and lysis, neutralization, wash), spin columns

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Quantum Prep® Plasmid Midiprep Kit

7326120
20 preps, large quantity plasmid DNA purification kit, includes Quantum Prep matrix, solutions (cell resuspension and lysis, neutralization, wash), midi spin columns

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Number Description Options
2559 Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities Click to download
2325 Quantum Prep Plasmid Midi- and Maxiprep Kit Product Information Sheet Click to download
2277 Quantum Prep Plasmid Miniprep Kit Product Information Sheet Click to download
4100066 Instruction Manual, Quantum Prep Plasmid Miniprep Kit, Rev G Click to download
4100111 Instruction Manual, Quantum Prep Plasmid Midiprep Kit, Rev E Click to download

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