Overview
Description
Description
The easy-to-use Aurum plasmid mini kit improves the efficiency and throughput of plasmid purifications with a simple bind-wash-elute protocol using silica membranes. Lysed bacterial cultures can be cleared either by centrifugation or by vacuum filtration with the Aurum vacuum manifold. The clarified lysates are then applied to plasmid binding columns and plates, where DNA is bound, then washed, and finally eluted — all in <10 minutes. The purified plasmid DNA can be immediately used in any downstream molecular biology application.
The Aurum plasmid mini kit delivers high yields of reproducible plasmid DNA preparations for:
- Automated fluorescent sequencing
- Restriction digestion
- Ligation and transformation
For more information, request bulletin 2664.
Specifications
Specifications
Kit contains sufficient reagents to purify DNA from 100 minicultures of plasmid-bearing bacteria.
DNA-binding mini columns
100
Capless collection tubes
100
Resuspension solution, 25 ml
1
Lysis solution, 25 ml
1
Neutralization solution, 40 ml
1
5x wash solution, 25 ml
1
Elution solution, 16 ml
1
Instruction manual
1
DNA yield
>20 µg
Preparation time
10 min
A200/A280
1.75–1.85
Bacterial density
Up to OD 12
Purity
>99% accuracy
Purification format
Vacuum and spin
Ordering
Ordering
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(Discontinued) Aurum™ Plasmid Mini Kit
7326400
100 preps, plasmid purification preparation kit, includes plasmid-binding mini columns, capless collection tubes, reagents, protocol overview
Documents
Documents
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