Aurum™ Plasmid Mini Kit

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Aurum Plasmid Mini Kit

Overview

Plasmid Purification Kit Selection Guide
Kit DNA
Yield
Preparation
Time
Bacterial Culture
Density or Volume
Purification
Format
Binding
Matrix
Growth
Media
Aurum plasmid mini >20µg 10 min Up to 12 OD • ml* Vacuum**
and spin
Silica
membrane
Standard
Quantum Prep miniprep >20µg 15 min 1–10 ml Spin Diatomaceous
earth
Enriched
or standard

* (OD600 of undiluted culture) x (culture volume in ml) = #of OD • ml. 1 OD600 is equivalent to approximately 8 x 108 cells/ml.
** Using the Aurum vacuum manifold.

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The easy-to-use Aurum plasmid mini kit improves the efficiency and throughput of plasmid purifications with a simple bind-wash-elute protocol using silica membranes. Lysed bacterial cultures can be cleared either by centrifugation or by vacuum filtration with the Aurum vacuum manifold. The clarified lysates are then applied to plasmid binding columns and plates, where DNA is bound, then washed, and finally eluted — all in <10 minutes. The purified plasmid DNA can be immediately used in any downstream molecular biology application.

The Aurum plasmid mini kit delivers high yields of reproducible plasmid DNA preparations for:

  • Automated fluorescent sequencing
  • Restriction digestion
  • Ligation and transformation

For more information, request bulletin 2664.

Kit contains sufficient reagents to purify DNA from 100 minicultures of plasmid-bearing bacteria.  
DNA-binding mini columns 100
Capless collection tubes 100
Resuspension solution, 25 ml 1
Lysis solution, 25 ml 1
Neutralization solution, 40 ml 1
5x wash solution, 25 ml 1
Elution solution, 16 ml 1
Instruction manual 1
DNA yield >20 µg
Preparation time 10 min
A200/A280 1.75–1.85
Bacterial density Up to OD 12
Purity >99% accuracy
Purification format Vacuum and spin
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Aurum™ Plasmid Mini Kit

7326400
100 preps, plasmid purification preparation kit, includes plasmid-binding mini columns, capless collection tubes, reagents, protocol overview

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(Discontinued) Aurum™ Vacuum Manifold

7326470
Vacuum-mediated nucleic acid purification platform for spin columns or 96-well plates, includes column adaptor plate and all necessary accessories

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
2664 Aurum Plasmid Mini Kit Product Information Sheet, Rev C Click to download
2671 Quick Guide, Aurum Vacuum Manifold Column Purification, Rev A Click to download
2663 Quick Guide, Aurum Plasmid Mini Kit Spin Format Protocol Overview, Rev A Click to download
4110111 Instruction Manual, Aurum Plasmid Mini Kit, Biotechnology Explorer, Rev C Click to download

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