ProteoMiner Protein Enrichment Kits

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Overview

ProteoMiner protein enrichment technology employs a large bead-based library of combinatorial peptide ligands, which dilutes high-abundance proteins and concentrates low-abundance proteins.
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Description

Description

ProteoMiner protein enrichment technology employs a large, highly diverse bead-based library of combinatorial peptide ligands, which simultaneously dilutes high-abundance proteins and concentrates low-abundance proteins. When a complex biological sample is applied to the beads, high-abundance proteins saturate their high-affinity ligand(s) and excess protein is washed away. In contrast, low-abundance proteins are concentrated on their specific affinity ligand(s).

ProteoMiner protein enrichment kits:

  • Decrease the amount of high-abundance proteins without immunodepletion, preventing the loss of proteins bound to high-abundance proteins.
  • Enrich medium- and low-abundance proteins that cannot be detected through traditional methods.
  • Do not rely on a predefined set of antibodies, unlike immunodepletion products.
  • Are compatible with a variety of sample types.
  • Offer a convenient, easy-to-use format.
  • Can be used for differential expression analysis.

 

Multiple Kits Available
A small-capacity kit is available for processing ≥10 mg of protein, while a large-capacity kit is available for processing ≥50 mg of protein. These kits have been optimized for use with serum and plasma samples, but they may be used with other sample types within the recommended total protein range.


The small- and large-capacity kits are available in two formats:

  • ProteoMiner protein enrichment kits — provide all necessary reagents for accessing low-abundance proteins, utilizing a single elution reagent in a simple, easy-to-perform process.
  • ProteoMiner sequential elution kits — utilize multiple elution reagents to sequentially elute proteins based on different properties. These kits fractionate proteins to improve detection and resolution and have been optimized for SELDI experiment samples (reagents are not compatible for direct use with 2-D gel electrophoresis).

 

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items

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1633003
10 preps, protein preparation reagents for eluting proteins into multiple fractions, columns not included, for use with #1633006 and #1633007
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1633006
10 preps, low-abundance protein enrichment kit for 10 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes
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1633007
10 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes
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1633008
2 preps, low-abundance protein enrichment kit for 10 mg of total protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes
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1633009
2 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes
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1633010
10 preps, protein preparation kit for sequential elution into 4 fractions, for 10 mg of total protein, includes 10 spin columns, buffers, collection tubes
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1633011
10 preps, protein preparation kit for sequential elution into 4 fractions, for 50 mg of total protein, includes 10 spin columns, buffers, collection tubes

Accessories

Accessories

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7326207
Pkg of 100, empty polypropylene spin columns, 1.2 ml bed volume, disposable, autoclavable

Documents

Documents

Number Description Options
5993
Comparative Proteomics of Umbilical Cord and Maternal Plasma, Rev A
5632
Accessing Low-Abundance Proteins in Serum and Plasma With a Novel, Simple Enrichment and Depletion Method, Rev B
3096
Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B
5635
ProteoMiner Protein Enrichment Technology Bulletin, Rev B
5835
ProteoMiner™ Sequential Elution Kit Protocol, Rev A
5841
Quantitation of Serum and Plasma Proteins After Enrichment of Low-Abundance Proteins With the ProteoMiner Protein Enrichment System, Rev A
10010636
Instruction Manual, ProteoMiner Protein Enrichment Kits, Rev D
10011083
ProteoMiner Protein Enrichment and Sequential Kit Protocols, Rev A
6441
2-D Analysis of Leaf Protein Samples Treated with ProteoMiner Beads Under Denaturing and Nondenaturing Conditions Poster, Rev A