Protein Stains

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Overview

Proteins in electrophoresis gels can be seen using a variety of protein-specific stains. Bio-Rad offers several sensitive stains that are ideal for classroom...
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Description

Description



Protein Stains

Proteins in electrophoresis gels can be seen using a variety of protein-specific stains. Bio-Rad offers several sensitive stains that are ideal for classroom use.

Bio-Safe Coomassie Stain

By far our most popular stain for research and teaching labs, Bio-Safe Coomassie stain detects proteins in native, SDS-PAGE, and peptide gels without methanol, acetic acid, or other hazardous reagents. Bio-Safe Coomassie stain is a ready-to-use, single-reagent protein stain that produces a linear response, allowing quantitative analysis. Color development is extremely easy. Simply soak the gel in the stain and rinse with water; results begin to appear in 20 min. With sensitivity as low as 8 ng per band, you simply get better results without the solvent waste and toxicity problems of conventional protein stains.

Coomassie (Brilliant) Blue R-250 Solutions

Coomassie (Brilliant) Blue R-250 staining solution is the easiest way to fix and detect proteins in polyacrylamide gels. Ready-to-use solutions are packaged in 1 L sizes and eliminate the need to weigh powders or dilute solutions.

Silver Stain Plus™ Kit

Easy-to-use Silver Stain Plus stain is our most sensitive silver stain. The Silver Stain Plus kit is ideal for both proteins and nucleic acids in polyacrylamide or agarose gels.

Key Features

  • Detects nanogram quantities of protein and DNA
  • Eliminates gel background by preventing silver precipitation in the gel matrix
  • Involves only three steps: fixing, washing, and staining
  • Detects bands in 1 hr
  • Stains 40 mini gels per kit

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1610435EDU
Kit for staining protein-containing polyacrylamide gels, includes 1 L Coomassie Brilliant Blue R-250 staining solution and 2 x 1 L destaining solution; education use only
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1610449EDU
Kit for detection of nanogram quantities of DNA and protein in polyacrylamide and agarose gels, for 13 full-size or 40 mini gels; education use only

Documents

Documents

Number Description Options
LIT442
Instruction Manual, Silver Stain Plus™, Rev E
2423
Bio-Safe Coomassie Stain Brochure, Rev D
4307051
Instructions for Staining Polyacrylamide Gels, Rev B