Protein Assay Kits

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Overview

A number of assays are available for the determination of protein concentration. The Bio-Rad protein and Quick Start Bradford protein assays are recommended for classroom use.
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Description

Description

A number of assays are available for the determination of protein concentration, each with a set of advantages. The Bio-Rad protein and Quick Start™ Bradford protein assays are recommended for classroom use.

Bio-Rad Protein Assay Kit

The simplicity, sensitivity, and color of the Bio-Rad protein assay make it a great vehicle for integrating biology, math, experimental design, and technique. The assay is derived from the Bradford method (1976) and is based on the color change of Coomassie (Brilliant) Blue G-250 dye in direct response to various concentrations of protein. In a typical application, the protein assay reagent is added to a sample and the color change is quantitated with a spectrophotometer or microplate reader. Protein standards provided in this kit allow generation of a standard curve based on the color intensities by known concentrations of protein. The amount of protein in an unknown sample can also be accurately estimated by eye, making this a great low-tech alternative for the classroom. Advantages of the Bio-Rad protein assay kit include:

  • Storage up to 1 year at recommended 4°C
  • Protein standard included
  • Rapid and sensitive
  • Classroom alternative to Biuret reagent

Quick Start Bio-Rad Protein Assay Kit

The Quick Start Bradford protein assay quantitates your samples quickly so you can move on with your experiments. The ready-to-use Quick Start assay provides excellent linearity, compatibility with a wide range of reagents, and convenient features such as:

  • Prediluted standards
  • 1x dye reagent
  • Screwcap vials instead of ampoules
  • Standard microplate and microassay protocols

References

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254.

Ordering

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5000201EDU
Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard (5 x 2 mg/ml); 200 standard assays or 4,000 microplate assays; education use only
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5000202EDU
Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml); education use only
500-0203EDU_tn.jpg
5000203EDU
Colorimetric assay kit, includes 1x dye reagent (1 L), bovine γ-globulin standard (5 x 2 mg/ml); education use only
500-0204EDU_tn.jpg
5000204EDU
Colorimetric protein assay kit, includes 1x dye reagent (1 L), bovine γ-globulin standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml); education use only
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5000001EDU
Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine γ-globulin standard; for 440 standard assays or 2,200 microplate assays; education use only
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5000002EDU
Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine serum albumin standard; for 440 standard assays or 2,200 microplate assays; education use only
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5000006EDU
450 ml, colorimetric assay dye, concentrate, for use with Bio-Rad protein assay kits I and II (#500-0001EDU, 500-0002EDU); education use only

Accessories

Accessories

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2240096EDU
Box of 20 packages, 5 high binding enzyme immunoassay plates per package (100 plates), polystyrene; education use only

Documents

Documents

Number Description Options
4110065
Instruction Manual, Quick Start Bradford Protein Assay, Rev A
2969
Quick Start Bradford Protein Assay Product Information Sheet, Rev A
1123
Protein Assay Applications Bibliography
LIT33
Instruction Manual, Bio-Rad Protein Assay, Rev C
1069
Colorimetric Protein Assays, Rev D