PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit

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Overview

The PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit provides reproducible, quantitative results in wastewater testing for SARS-CoV-2, the virus caused by COVID-19.

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Description

Description

The PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit provides reproducible, quantitative results in wastewater testing for SARS-CoV-2, the virus caused by COVID-19.

The PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit, based on the U.S. CDC and WHO RT-PCR primers for SARS-CoV-2 detection, allows for the detection of N2 and E genes in SARS-CoV-2. It also includes an assay for the Murine Coronavirus or Murine Hepatitis Virus (MHV), an enveloped, single-stranded RNA virus. MHV serves as a matrix recovery control, enabling estimation of the virus lost during sample processing. The test allows for all three targets to be detected in a single well using the kit. The kit also contains reagents sufficient for 200 reactions, and there is an automated software tool available for analysis.

Features and Benefits

  • Addresses an unmet need in COVID surveillance — the ability to test millions of people with sufficient lead time to take action
  • Quantitative without requiring a standard curve
  • More inhibitor-resistant compared to qPCR
  • Reproducible results across runs, reagent lots, and instruments
  • Single-well multiplex test, or two two-well test if using fecal indicator
  • Turnaround time <8 hours from sample to answer
  • Validated on the QX200 ddPCR Platforms, compatible with QX ONE ddPCR Platform
  • Aligned to CDC guidelines on wastewater testing
  • Multiple viral targets for more robust assay performance
  • Automatic analysis with the PREvalence Wastewater Automated Analysis Application

Applications and Uses

  • Detecting SARS-CoV-2 virus in wastewater

Related Products

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12015402
200 reactions, includes ddPCR Triplex Assay (SARS-CoV-2 N2, E, and MHV), Supermix for One-Step RT ddPCR Advanced Mix for Probes, RT for One-Step RT ddPCR Advanced Mix for Probes, DTT, and positive control
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12015961
100 reactions, includes ddPCR crAssphage Fecal Indicator Assay used with the PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit (12015402)
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12015962
100 reactions, includes ddPCR PMMoV Fecal Indicator Assay used with the PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit (12015402)

Other

Other

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1863004
2 L (2 x 1 L), oil for use with droplet reader in the QX200™/QX100™ Droplet Digital™ PCR Systems
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1864108
Pkg of 30, cartridges for use with the Automated Droplet Generator in the QX200 AutoDG Droplet Digital PCR System
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1864120
Pkg of 20, boxes of 96 each, pipet tips for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System
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1864125
Pkg of 10, pipet tip waste bins for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System
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1864110
140 ml, oil for use with the Automated Droplet Generator for probe-based detection with the QX200 AutoDG Droplet Digital PCR System
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1814040
Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer
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MSF1001
Pkg of 100, aluminized foil seal for PCR plates, pierceable
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1864008
Pkg of 24, cartridges for use with the QX200™/QX100™ Droplet Generator
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1863009
Pkg of 24, gaskets for use with QX200™/QX100™ Droplet Generator
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(Discontinued) DG8 Cartridge Holder
1863051
Pkg of 1, cartridge holder, positions and holds the cartridge in the QX200 Droplet Generator
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1863005
70 ml (10 x 7 ml), oil for use with droplet generator in the QX200/QX100™ Droplet Digital™ PCR Systems

Instrument

Instrument

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(Discontinued) QX200 Droplet Reader
1864003
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
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1864101
AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX200/QX100; Droplet Digital PCR Systems
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1864002
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
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1851197
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive
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(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

Download

Download

QX Manager Software Standard Edition, Version 1.2
QX-MANAGER-V.1.2-STD
QX Manager Software Standard Edition, Version 1.2
PREvalence Wastewater Automated Analysis Application
Covid_WW_Interpretive_Application_1.0.0.6
Automate analysis with the PREvalence Wastewater Automated Analysis Application

Documents

Documents

Number Description Options
10000142158
PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit, User Guide
10000145551
PREvalence ddPCR crAssphage Fecal Indicator Assay, Product Insert
10000145552
PREvalence ddPCR PMMoV Fecal Indicator Assay, Product Insert