
Overview
Property | DEAE | High Q | High S | CM |
Type of support | Weak anion | Strong anion | Strong cation | Weak cation |
Functional ligand | –N+(C2H5)2 | –N+(CH3)3 | –SO3- | –COO- |
Ionic capacity | 175 ±75 µeq/ml | 400 ±75 µeq/ml | 160 ±40 µeq/ml | 210 ±40 µeq/ml |
Typical binding capacity | 35 mg BSA/ml | 40 mg BSA/ml | 70 mg IgG/ml | 35 mg hemoglobin/ml |
Counterion | Cl- | Cl- | Na+ | Na+ |
Nominal particle size | 50 µm | 50 µm | 50 µm | 50 µm |
Nominal pore size | 1,000 Å | 1,000 Å | 1,000 Å | 1,000 Å |
Recommended maximum linear flow rate | 3,000 cm/hr | 3,000 cm/hr | 3,000 cm/hr | 3,000 cm/hr |
Chemical stability | ||||
1% SDS, 24 hr | Yes | Yes | Yes | Yes |
6 M guanidine-HCl, 24 hr | Yes | Yes | Yes | Yes |
Volume changes | ||||
pH 4–10 | <1% | <1% | <3% | <1% |
0.1–1.0 M NaCl | <5% | <5% | <9% | <4% |
Autoclavability* (121°C, 80 min) | Yes | Yes | Yes | Yes |
pH stability | 1–10 | 1–10 | 1–12 | 1–12 |
Antimicrobial agent | 20% ethanol | 20% ethanol | 20% ethanol | 20% ethanol |
Regeneration | 1 M NaCl | 1 M NaCl | 1 M NaCl | 1 M NaCl |
* Do not autoclave the OH- form.
Scale | Packaging Format | |||||
Characteristics | Product Type | Analytical | Pilot/Preparative | Process | Prepacked | Bottled |
Ion Exchange Chromatography | ||||||
Separation by charge | UNOsphere™ media | ![]() | UNOsphere Q, S, Rapid S | |||
General biomolecules purification | Macro-Prep® media | ![]() | Macro-Prep High Q, DEAE, High S, CM | |||
Applicable to all antibody purifications | Macro-Prep 25 media | ![]() | Macro-Prep 25 Q, 25 S | |||
UNO® columns | ![]() | UNO Q, S | ||||
Bio-Scale™ Mini Cartridges | ![]() | UNOsphere Q, S | ||||
High capacity | Macro-Prep High Q, S | |||||
Good capture technique | AG® resins | ![]() | AG | |||
Chelex® resins | ![]() | Chelex | ||||
Bio-Rex® resins | ![]() | Bio-Rex | ||||
Hydroxyapatite and Fluoroapatite Chromatography | ||||||
Unique selectivity | CHT™ 80 µm support | ![]() | CHT, Types I and II | |||
Alternative to protein A for antibody purification | CHT 40 µm support | ![]() | CHT, Types I and II | |||
CHT 20 µm support | ![]() | CHT, Types I and II | ||||
CFT 40 µm support | ![]() | CFT, Types I and II | ||||
Removal of leached protein A, aggregates, endotoxins, and DNA | Bio-Gel® HT and HTP media | ![]() | Bio-Gel HT, HTP | |||
Bio-Scale columns | ![]() | CHT Type I (10 µm) | ||||
CHT Type II (20 µm) | ||||||
Affinity Chromatography | ||||||
Purification of His- and GST- tagged proteins | Profinity™ IMAC and GST media | ![]() | Bio-Scale Mini | Profinity IMAC | ||
Separation by biological interaction | Affi-Gel resins | ![]() | Affi-Gel® Protein A* | |||
Affi-Gel Blue* | ||||||
DEAE Affi-Gel Blue* | ||||||
CM Affi-Gel Blue* | ||||||
High selectivity | Affi-Gel boronate, heparin** | |||||
High purity in a single step | Affi-Gel Hz, 10, 15** | |||||
Affi-Gel 102** | ||||||
Excellent capture technique | Profinity epoxide media | ![]() | Profinity epoxide** | |||
Affi-Prep media | ![]() | Affi-Prep protein A* | ||||
Affi-Prep polymyxin* | ||||||
Bio-Scale Mini cartridges | ![]() | Profinity IMAC and GST | ||||
Econo-Pac® columns | ![]() | Affi-Gel Blue | ||||
DEAE Affi-Gel Blue | ||||||
Affi-Gel protein A | ||||||
Size Exclusion Chromatography | ||||||
Separation by size | Bio-Gel P media | ![]() | Bio-Gel P | |||
MW determination | Bio-Beads™ S-X media | ![]() | Bio-Beads S-X | |||
Applicable to all antibody purifications | Bio-Sil® and Bio-Silect™ columns | ![]() | Bio-Sil, Bio-Silect | |||
Desalting or buffer exchange | Bio-Scale Mini cartridges | ![]() | Bio-Gel P-6 | |||
High resolution | Econo-Pac columns | ![]() | Econo-Pac 10DG | |||
Hydrophobic Interaction Chromatography | ||||||
Separation by hydrophobicity | Macro-Prep HIC media | ![]() | Macro-Prep t-butly | |||
Macro-Prep methyl |
* Ready to use.
** Activated.
Type of Ion Exchanger | Buffer | Buffering Range |
Cation | Acetic acid | 4.8–5.2 |
Citric acid | 4.2–5.2 | |
HEPES | 6.8–8.2 | |
Lactic acid | 3.6–4.3 | |
MES | 5.5–6.7 | |
MOPS | 6.5–7.9 | |
Phosphate | 6.7–7.6 | |
PIPES | 6.1–7.5 | |
TES | 7.2–7.8 | |
Tricine | 7.8–8.9 | |
Anion | Bicine | 7.6–9.0 |
Bis-Tris | 5.8–7.2 | |
Diethanolamine | 8.4–8.8 | |
Diethylamine | 9.5–11.5 | |
L-histidine | 5.5–6.0 | |
Imidazole | 6.6–7.1 | |
Pyridine | 4.9–5.6 | |
Tricine | 7.4–8.8 | |
Triethanolamine | 7.3–8.3 | |
Tris | 7.5–8.0 |
Note:Buffer solution of 50 mM is recommended.
This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program
Call us at 1-800-4-BIORAD (1-800-424-6723)
I cannot find the Certificate of Analysis I am looking for. Help!
When using our online Certificate of Analysis search tool, keep the following in mind:
- Make sure you entered the correct catalog number and lot/control number in the search fields
- If you have a kit, try looking up the Certificate of Analysis using the kit information as well as the information for the individual components
- The Certificate of Analysis that you are looking for may not be available via the website. In that case, you can contact your Bio-Rad representative or use the Request Form
If you cannot find the Certificate of Analysis you are looking for, please Contact Us.
Where can I find the catalog number, SKU number, or product number?
The catalog number, SKU number, or product number is printed on your product label. See the sample label below for the location of this information.
Where can I find the lot or control number?
The lot number or control number (one or the other, not both) is printed on your product label. See the sample label below for the location of this information.

Why are Certificates of Analysis not on the Documents tab?
Certificates of Analysis are associated not only with a product, but also with a specific lot of that product. For a given product, there may be several Certificates of Analysis, especially if it is an older product line and has had several lots produced over the years. Using the Certificate of Analysis search tool, you can enter the catalog number and lot/control number for the specific product you have on hand and download the exact Certificate of Analysis you need.
I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?
You can use the batch number in place of the lot/control number. Use the Certificate of Analysis search tool, entering the catalog number as usual and the batch number where the lot/control number would go.
Can I get a Certificate of Analysis even if my item is expired?
Yes. While we do periodically remove Certificates of Analysis for site maintenance purposes, we typically keep them available well beyond a product's expiration date.
Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?
There are Certificates of Analysis that correspond to products that are either discontinued or simply not available on the website. In these situations, the Certificate of Analysis is available for download, but other product details, such as the product name, are not.
Why does it say "Not Available" for a Certificate of Analysis listed in the search results?
In this case, you have entered a valid catalog number and lot/control number and we have located the Certificate of Analysis. However, for some reason, the actual file itself is unavailable.
Please use the Request Form here or contact your local Bio-Rad Representative so that we can send you the Certificate of Analysis.
Please reenter your email address in the correct format.
Please enter your email address.
Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile.
To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. You can elect to receive only the types of Bio-Rad communications that are of interest to you..
Our Products
Home |
Trademarks |
Site Terms |
Terms & Conditions |
Privacy
© 2019 Bio-Rad Laboratories, Inc.