Criterion™ TBE-Urea Precast Gels

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Overview

TBE-urea gels maintain denaturing conditions for analysis of single-stranded DNA and RNA between 60 and 200 bases. Designed for the traditional 13.3 x 8.7 cm mini vertical format.
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Description

Description

TBE-urea gels maintain denaturing conditions for analysis of single-stranded DNA and RNA. Nucleic acids between 60 and 200 bases are resolved as sharp, distinct bands. Applications include oligonucleotide purity analysis, RNase protection assays, and northern blotting.

Criterion™ precast gels include a broad selection of 13.3 x 8.7 cm polyacrylamide gels in single-use cassettes. This gel size provides reproducible, high-resolution results with fast setup, loading, and run times. Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion™ Dodeca™ cell. The Criterion blotter offers a variety of convenient features, such as two electrode configurations and a convenient gel/blot assembly tray, for blotting applications.

Specifications

Specifications

Gel dimensions
13.3 x 8.7 cm (W x L); 1.0 mm thick
Cassette dimensions
15.0 x 10.6 cm (W x L); 5.3 mm thick
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Storage tray material
PET
Gel storage conditions
Store flat at 4°C; do not freeze
Gel shelf life*
12 weeks for Tris-HCl, Tris-Tricine, zymogram, TBE, TBE-urea gels
Buffer volume
Upper, 60 ml; lower, 400 ml
* From date of manufacture.
 

Ordering

Ordering

items

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3450086
Pkg of 1, 5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450088
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0089_tn.jpg
3450089
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0090_tn.jpg
3450090
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0091_tn.jpg
3450091
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0092_tn.jpg
3450092
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0093_tn.jpg
3450093
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Kits & Reagents

Kits & Reagents

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1610767
10 ml, 5x DNA loading buffer, premixed, for nondenaturing TBE gels, pH 8.0
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1610768
30 ml, 2x TBE-urea sample loading buffer, premixed, for denaturing ssDNA and RNA, pH 8.0
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1610770
5 L cube, TBE nucleic acid electrophoresis buffer solution, pH 8.3

Documents

Documents

Number Description Options
2788
Criterion Blotting Tray Insert, Rev A
2710
Criterion™ Precast Gel System Brochure, Rev C