CHT Ceramic Hydroxyapatite XT Media

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Overview

CHT XT Media is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. It is fine-tuned for the removal of aggregates and process impurities.

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Description

Description

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CHT Ceramic Hydroxyapatite XT Media (CHT XT) is a calcium affinity cation exchange chromatography media. It is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. The chemical composition of CHT XT is (Ca10(PO4)6(OH)2) and it is a mixed-mode media. Biomolecules can interact with CHT through calcium affinity interactions and/or cation exchange interactions. It provides unparalleled separation properties and resolution. It can be used in applications such as purification of enzymes, monoclonal and polyclonal antibodies of various classes, antibody fragments, and bispecific antibodies; efficient isolation and purification of viruses and virus-like particles (VLPs); and separation of supercoiled DNA from linear duplexes as well as single stranded from double-stranded DNA. It is ideal for large scale bioprocess applications.

CHT XT Media delivers

  • Unique selectivity
  • Efficient single-step clearance of aggregates and other impurities
  • High physical and chemical stability
  • Straightforward column packing
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Learn Best Practices and Guidelines for Packing CHT Media

These guidelines for handling and packing are recommended for all CHT users to ensure reproducible and high-quality results. Please download the CHT Application Guide for specific details on recommended buffers and slurry calculations.

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Product Formats

Product Catalog Number
CHT XT Media, 10 g 12002457
CHT XT Media, 100 g 12002454
CHT XT Media, 1 kg 12002456
CHT XT Media, 5 kg 12002455

Screening Tools

  • Foresight CHT XT Column, 1 ml

    Foresight CHT XT Column, 1 ml

    12003150

  • Foresight CHT XT Column, 5 ml

    Foresight CHT XT Column, 5 ml

    12003149

  • Foresight CHT XT, 200 μl Robocolumn

    Foresight CHT XT, 200 μl Robocolumn

    12003152

  • Foresight CHT XT, 600 μl Robocolumn

    Foresight CHT XT, 600 μl Robocolumn

    12003148

  • Foresight CHT XT, 20 μl Plates

    Foresight CHT XT, 20 μl Plates

    12003151

Specifications

Specifications

Property
Description
Function
Mixed-mode: cation exchange (phosphate) and affinity (calcium)
Functional group
Ca2+, PO4, OH
Median particle size
40 ± 4 µm
Dynamic binding capacity
17–25 mg Lysozyme/g*
>60 mg/ml mAb G** (pI 9.2)
* At 500 cm/hr with 10 mM sodium phosphate, pH 6.8.
** At 100 cm/hr with 5 mM sodium phosphate, 25 mM sodium chloride, pH 7 in a 0.5 x 9.5 cm column
Recommended linear flow rate
50–300 cm/hr
Tested on a 0.4 x 10 cm column packed at 150 cm/hr
Maximum operating pressure
10 bar (150 psi)
Tap settled density
0.67 g/ml
Compression factor
Incompressible
   pH stability
6.5–14
Shipping form
Dry powder
Regeneration
0.4 to 0.5 M sodium phosphate, pH 7.0-7.5; 1 M trisodium phosphate, pH 11-12. If higher concentrations of sodium phosphate are needed, use 0.4-1.0 M potassium phosphate.
Sanitization
1-2 N sodium hydroxide
Storage conditions
0.1 N sodium hydroxide
Autoclavability
121°C, 20 min in phosphate buffered saline, pH 7
Chemical compatibility
1 N sodium hydroxide, 8 M Urea, 6 M guanidine hydrochloride, ethanol, methanol, 100% acetonitrile, 0.4–1.0 M phosphate buffer, 0.5 M sodium phosphate, pH 7, 1.0 M trisodium phosphate, pH 11–12
Shelf life
5 years

Ordering

Ordering

items

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12009261

Pkg of 1, 1 ml, prepacked mixed mode column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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12002457
Pkg of 1, 10 g CHT XT mixed-mode chromatography media, 40 µm particle size
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12002454
Pkg of 1, 100 g CHT XT mixed-mode chromatography media, 40 µm particle size
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12002456
Pkg of 1, 1 kg CHT XT mixed-mode chromatography media, 40 µm particle size
12002455_tn.jpg
12002455
Pkg of 1, 5 kg CHT XT mixed-mode chromatography media, 40 µm particle size
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12003149
Pkg of 1, 5 ml column prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size
12003150-thumb.jpg
12003150
Pkg of 1, 1 ml column prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size
12003151-thumb.jpg
12003151
2 x 96-well plates, 20 µl, filter plate prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size
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12003152
Pkg of 1, row of 8 columns, 200 µl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Media, 40 µm particle size
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12003148
Pkg of 1, row of 8 columns, 600 µl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Media, 40 µm particle size

Documents

Documents

Number Description Options
3225
Achieve High-Level Impurity Clearance during mAb Purification with a Single Media Flier
[ Add to Cart (Free) ]
6895
Resin Functionalities Maximize Process Value For Biomolecule Purifications
[ Add to Cart (Free) ]
7109
Removing the Aggregates of an Acidic Monoclonal Antibody with CHT Ceramic Hydroxyapatite XT Media Application Note
7115
Single-Step Influenza and Dengue Virus Purification with Mixed-Mode CHT Ceramic Hydroxyapatite XT Media Application Note
6982
Mixed-Mode Resins/Media Selection Guide
[ Add to Cart (Free) ]
7099
Improve mAb Purification Cost Efficiency With the Right Resin
[ Add to Cart (Free) ]
7080
CHT™ Ceramic Hydroxyapatite XT Media Product Information Sheet
6086
Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up
[ Add to Cart (Free) ]
6965
Recommendations for CHT™ Ceramic Hydroxyapatite Column Packing (Poster)
6932
Good Practices for CHT™ Ceramic Hydroxyapatite Media Usage (Poster), Ver A
6902
How Does CHT™ Ceramic Hydroxyapatite Media Work (Poster), Ver A
6886
Quick Monoclonal Antibody Purification Strategy: Affinity-Based Capture Flier, Ver A
6946
Monoclonal Antibody Purification: Polish Purification Resins Brochure, Ver A
6870
Ceramic Apatite-Based Chromatography Media Publications List, Ver A
7382
Purification of a Recombinant Monoclonal Antibody — a Rituximab Biosimilar — with a Non–Affinity Based Chromatographic Process