CHT Ceramic Hydroxyapatite XT Media

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CHT Ceramic Hydroxyapatite XT Media

Overview

CHT™ Media Maximizes Purity and Recovery in Downstream Processing

This whiteboard animation video provides a brief introduction to Bio-Rad’s innovative mixed-mode chromatography media, CHT Ceramic Hydroxyapatite. The unique separation properties and selectivity of CHT Media enable you to maximize purity and recovery in a single step of downstream purification for the development of biotherapeutics.

 

Process-Scale Packing Guidelines: CHT™ Ceramic Hydroxyapatite
Optimized Purity of a mAb using Mixed-Mode Media

Dr. Carsten Voß, Application Specialist, Process Chromatography, Bio-Rad Laboratories GmbH, reviews the important factors and parameters when screening multiple chromatography media (resins) for the development of efficient, robust, and economical production processes of biotherapeutics such as monoclonal antibodies. Using a bind-and-elute strategy provides enhanced purification power compared to flow-through modes in this discussion of evaluating multiple mixed-mode resins for the purification of mAbs. CHT Ceramic Hydroxyapatite Mixed-Mode Media provided the best monomer recovery in the smallest elution volume at a target purity of 99.5%.

CHT XT Media is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. It is fine-tuned for the removal of aggregates and process impurities.


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Description

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FREE Ceramic hydroxyapatite reference wall charts

Free Ceramic Hydroxyapatite reference wall charts for process purification

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CHT Ceramic Hydroxyapatite XT Media (CHT XT) is a calcium affinity cation exchange chromatography media. It is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. The chemical composition of CHT XT is (Ca10(PO4)6(OH)2) and it is a mixed-mode media. Biomolecules can interact with CHT through calcium affinity interactions and/or cation exchange interactions. It provides unparalleled separation properties and resolution. It can be used in applications such as purification of enzymes, monoclonal and polyclonal antibodies of various classes, antibody fragments, and bispecific antibodies; efficient isolation and purification of viruses and virus-like particles (VLPs); and separation of supercoiled DNA from linear duplexes as well as single stranded from double-stranded DNA. It is ideal for large scale bioprocess applications.

CHT XT Media delivers

  • Unique selectivity
  • Efficient single-step clearance of aggregates and other impurities
  • High physical and chemical stability
  • Straightforward column packing
FREE Ceramic hydroxyapatite reference wall charts

Learn Best Practices and Guidelines for Packing CHT Media

These guidelines for handling and packing are recommended for all CHT users to ensure reproducible and high-quality results. Please download the CHT Application Guide for specific details on recommended buffers and slurry calculations.

Watch Videos | Download Application Guide

Product Formats

Product Catalog Number
CHT XT Media, 10 g 12002457
CHT XT Media, 100 g 12002454
CHT XT Media, 1 kg 12002456
CHT XT Media, 5 kg 12002455

Screening Tools

  • Foresight CHT XT Column, 1 ml

    Foresight CHT XT Column, 1 ml

    12003150

  • Foresight CHT XT Column, 5 ml

    Foresight CHT XT Column, 5 ml

    12003149

  • Foresight CHT XT, 200 μl Robocolumn

    Foresight CHT XT, 200 μl Robocolumn

    12003152

  • Foresight CHT XT, 600 μl Robocolumn

    Foresight CHT XT, 600 μl Robocolumn

    12003148

  • Foresight CHT XT, 20 μl Plates

    Foresight CHT XT, 20 μl Plates

    12003151


Specifications

Property Description
Function Mixed-mode: cation exchange (phosphate) and affinity (calcium)
Functional group Ca2+, PO4, OH
Median particle size 40 ± 4 µm
Dynamic binding capacity 17–25 mg Lysozyme/g*
>60 mg/ml mAb G** (pI 9.2)
* At 500 cm/hr with 10 mM sodium phosphate, pH 6.8.
** At 100 cm/hr with 5 mM sodium phosphate, 25 mM sodium chloride, pH 7 in a 0.5 x 9.5 cm column
Recommended linear flow rate 50–300 cm/hr
Tested on a 0.4 x 10 cm column packed at 150 cm/hr
Maximum operating pressure 10 bar (150 psi)
Tap settled density 0.67 g/ml
Compression factor Incompressible
   pH stability 6.5–14
Shipping form Dry powder
Regeneration 0.4 to 0.5 M sodium phosphate, pH 7.0-7.5; 1 M trisodium phosphate, pH 11-12. If higher concentrations of sodium phosphate are needed, use 0.4-1.0 M potassium phosphate.
Sanitization 1-2 N sodium hydroxide
Storage conditions 0.1 N sodium hydroxide
Autoclavability 121°C, 20 min in phosphate buffered saline, pH 7
Chemical compatibility 1 N sodium hydroxide, 8 M Urea, 6 M guanidine hydrochloride, ethanol, methanol, 100% acetonitrile, 0.4–1.0 M phosphate buffer, 0.5 M sodium phosphate, pH 7, 1.0 M trisodium phosphate, pH 11–12
Shelf life 5 years

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EconoFit CHT XT, 40 μm Column, 1 x 1 ml

12009261
Pkg of 1, 1 ml, prepacked mixed mode column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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$49.32 
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CHT™ Ceramic Hydroxyapatite XT, 40 µm

12002457
Pkg of 1, 10 g CHT XT mixed-mode chromatography media, 40 µm particle size

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$210.00 
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CHT™ Ceramic Hydroxyapatite XT, 40 µm

12002454
Pkg of 1, 100 g CHT XT mixed-mode chromatography media, 40 µm particle size

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$1,150.00 
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CHT Ceramic Hydroxyapatite XT, 40 µm, 1 kg

12002456
Pkg of 1, 1 kg CHT XT mixed-mode chromatography media, 40 µm particle size

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CHT™ Ceramic Hydroxyapatite XT, 40 µm

12002455
Pkg of 1, 5 kg CHT XT mixed-mode chromatography media, 40 µm particle size

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Foresight™ CHT™ XT Column, 5 ml

12003149
Pkg of 1, 5 ml column prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size

List Price: 
 
$455.00 
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Foresight™ CHT™ XT Column

12003150
Pkg of 1, 1 ml column prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size

List Price: 
 
$230.00 
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Foresight™ CHT™ XT Plates, 20 µl

12003151
2 x 96-well plates, 20 µl, filter plate prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size

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$900.00 
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Foresight™ CHT™ XT RoboColumn, 200 µl

12003152
Pkg of 1, row of 8 columns, 200 µl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Media, 40 µm particle size

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$380.00 
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Foresight™ CHT™ XT RoboColumn, 600 µl

12003148
Pkg of 1, row of 8 columns, 600 µl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Media, 40 µm particle size

List Price: 
 
$440.00 
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Documents

Number Description Options
3225 Achieve High-Level Impurity Clearance during mAb Purification with a Single Media Flier Click to download [ Add to Cart (Free) ]
6895 Resin Functionalities Maximize Process Value For Biomolecule Purifications Click to download [ Add to Cart (Free) ]
7109 Removing the Aggregates of an Acidic Monoclonal Antibody with CHT Ceramic Hydroxyapatite XT Media Application Note Click to download
7115 Single-Step Influenza and Dengue Virus Purification with Mixed-Mode CHT Ceramic Hydroxyapatite XT Media Application Note Click to download
6982 Mixed-Mode Resins/Media Selection Guide Click to download [ Add to Cart (Free) ]
7099 Improve mAb Purification Cost Efficiency With the Right Resin Click to download [ Add to Cart (Free) ]
7080 CHT™ Ceramic Hydroxyapatite XT Media Product Information Sheet Click to download
6086 Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up Click to download [ Add to Cart (Free) ]
6965 Recommendations for CHT™ Ceramic Hydroxyapatite Column Packing (Poster) Click to download
6932 Good Practices for CHT™ Ceramic Hydroxyapatite Media Usage (Poster), Ver A Click to download
6902 How Does CHT™ Ceramic Hydroxyapatite Media Work (Poster), Ver A Click to download
6886 Quick Monoclonal Antibody Purification Strategy: Affinity-Based Capture Flier, Ver A Click to download
6946 Monoclonal Antibody Purification: Polish Purification Resins Brochure, Ver A Click to download
6870 Ceramic Apatite-Based Chromatography Media Publications List, Ver A Click to download
7382 Purification of a Recombinant Monoclonal Antibody — a Rituximab Biosimilar — with a Non–Affinity Based Chromatographic Process Click to download

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