CHT Ceramic Hydroxyapatite XT Media

Overview

Optimized Purity of a mAb using Mixed-Mode Media

CHT XT Media is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. It is fine-tuned for the removal of aggregates and process impurities.

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Description

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CHT Ceramic Hydroxyapatite XT Media (CHT XT) is a calcium affinity cation exchange chromatography media. It is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. The chemical composition of CHT XT is (Ca₁₀(PO₄)₆(OH)₂) and it is a mixed-mode media. Biomolecules can interact with CHT through calcium affinity interactions and/or cation exchange interactions. It provides unparalleled separation properties and resolution. It can be used in applications such as purification of enzymes, monoclonal and polyclonal antibodies of various classes, antibody fragments, and bispecific antibodies; efficient isolation and purification of viruses and virus-like particles (VLPs); and separation of supercoiled DNA from linear duplexes as well as single stranded from double-stranded DNA. It is ideal for large scale bioprocess applications.

CHT XT Media delivers

Unique selectivity
Efficient single-step clearance of aggregates and other impurities
High physical and chemical stability
Straightforward column packing

FREE Ceramic hydroxyapatite reference wall charts

Learn Best Practices and Guidelines for Packing CHT Media

These guidelines for handling and packing are recommended for all CHT users to ensure reproducible and high-quality results. Please download the CHT Application Guide for specific details on recommended buffers and slurry calculations.

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More Information

CHT Ceramic Hydroxyapatite XT Product Information Sheet
CHT Ceramic Hydroxyapatite XT Manual

Product Formats

Product	Catalog Number
CHT XT Media, 10 g	12002457
CHT XT Media, 100 g	12002454
CHT XT Media, 1 kg	12002456
CHT XT Media, 5 kg	12002455

Screening Tools

Foresight CHT XT Column, 1 ml

12003150
Foresight CHT XT Column, 5 ml

12003149
Foresight CHT XT, 200 μl Robocolumn

12003152
Foresight CHT XT, 600 μl Robocolumn

12003148
Foresight CHT XT, 20 μl Plates

12003151

Specifications

Property

Description

Function

Mixed-mode: cation exchange (phosphate) and affinity (calcium)

Functional group

Ca²⁺, PO₄, OH

Median particle size

40 ± 4 µm

Dynamic binding capacity

17–25 mg Lysozyme/g*
>60 mg/ml mAb G** (pI 9.2)
* At 500 cm/hr with 10 mM sodium phosphate, pH 6.8.
** At 100 cm/hr with 5 mM sodium phosphate, 25 mM sodium chloride, pH 7 in a 0.5 x 9.5 cm column

Recommended linear flow rate

50–300 cm/hr
Tested on a 0.4 x 10 cm column packed at 150 cm/hr

Maximum operating pressure

10 bar (150 psi)

Tap settled density

0.67 g/ml

Compression factor

Incompressible

pH stability

6.5–14

Shipping form

Dry powder

Regeneration

0.4 to 0.5 M sodium phosphate, pH 7.0-7.5; 1 M trisodium phosphate, pH 11-12. If higher concentrations of sodium phosphate are needed, use 0.4-1.0 M potassium phosphate.

Sanitization

1-2 N sodium hydroxide

Storage conditions

0.1 N sodium hydroxide

Autoclavability

121°C, 20 min in phosphate buffered saline, pH 7

Chemical compatibility

1 N sodium hydroxide, 8 M Urea, 6 M guanidine hydrochloride, ethanol, methanol, 100% acetonitrile, 0.4–1.0 M phosphate buffer, 0.5 M sodium phosphate, pH 7, 1.0 M trisodium phosphate, pH 11–12

Shelf life

5 years