
Description
Description
Aurum ion exchange mini kits utilize ion exchange chromatography to selectively enrich either acidic or basic proteins in less than 30 minutes. These easy-to-use kits include the columns and buffers for cation exchange (CEX) or anion exchange (AEX) chromatography and can be used with a variety of starting samples. Isolated proteins are suitable for most downstream proteomic applications, including 2-D gel analysis and mass spectrometry.
Aurum CEX | Aurum AEX | |
Type of support | UNOsphere S | UNOsphere Q |
Binding Capacity | 12 mg protein/column* | 36 mg protein/column* |
Column bed volume | 0.2 ml | 0.2 ml |
Binding buffer | 20 mM sodium acetate, pH 5.0 | 20 mM Tris, pH 8.3 |
Elution buffer | Binding buffer + 1.0 M NaCl | Binding buffer + 1.0 M NaCl |
* Binding capacity determined using IgG (CEX) and BSA (AEX). Capacity will differ slightly depending on the protein. |
Aurum ion exchange mini kits:
- Use a patented* polymerization technology to achieve ultrahigh protein binding capacity
- Accommodate up to 1 ml of sample
- Use a quick, easy, spin-column format
- Work on a variety of sample sources (serum, plasma, mammalian tissues or cells, plant leaves, E. coli, yeast)
*U.S. patent 6,423,666.
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