Aurum™ Ion Exchange Mini Kits

Aurum ion exchange mini kits utilize ion exchange chromatography to selectively enrich either acidic or basic proteins in less than 30 minutes.

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Overview

Aurum ion exchange mini kits utilize ion exchange chromatography to selectively enrich either acidic or basic proteins in less than 30 minutes. These easy-to-use kits include the columns and buffers for cation exchange (CEX) or anion exchange (AEX) chromatography and can be used with a variety of starting samples. Isolated proteins are suitable for most downstream proteomic applications, including 2-D gel analysis and mass spectrometry.

	Aurum CEX	Aurum AEX
Type of support	UNOsphere S	UNOsphere Q
Binding Capacity	12 mg protein/column*	36 mg protein/column*
Column bed volume	0.2 ml	0.2 ml
Binding buffer	20 mM sodium acetate, pH 5.0	20 mM Tris, pH 8.3
Elution buffer	Binding buffer + 1.0 M NaCl	Binding buffer + 1.0 M NaCl
* Binding capacity determined using IgG (CEX) and BSA (AEX). Capacity will differ slightly depending on the protein.

Aurum ion exchange mini kits:

Use a patented* polymerization technology to achieve ultrahigh protein binding capacity
Accommodate up to 1 ml of sample
Use a quick, easy, spin-column format
Work on a variety of sample sources (serum, plasma, mammalian tissues or cells, plant leaves, E. coli, yeast)

*U.S. patent 6,423,666.

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