Aurum™ Ion Exchange Mini Kits

Image
lsr_aurum_ion_ex_mini_kits.jpg

Overview

Aurum ion exchange mini kits utilize ion exchange chromatography to selectively enrich either acidic or basic proteins in less than 30 minutes.
Read full description Ordering Information

Description

Description

Aurum ion exchange mini kits utilize ion exchange chromatography to selectively enrich either acidic or basic proteins in less than 30 minutes. These easy-to-use kits include the columns and buffers for cation exchange (CEX) or anion exchange (AEX) chromatography and can be used with a variety of starting samples. Isolated proteins are suitable for most downstream proteomic applications, including 2-D gel analysis and mass spectrometry.

  Aurum CEX Aurum AEX
Type of support UNOsphere S UNOsphere Q
Binding Capacity 12 mg protein/column* 36 mg protein/column*
Column bed volume 0.2 ml 0.2 ml
Binding buffer 20 mM sodium acetate, pH 5.0 20 mM Tris, pH 8.3
Elution buffer Binding buffer + 1.0 M NaCl Binding buffer + 1.0 M NaCl
* Binding capacity determined using IgG (CEX) and BSA (AEX). Capacity will differ slightly depending on the protein.

Aurum ion exchange mini kits:

  • Use a patented* polymerization technology to achieve ultrahigh protein binding capacity
  • Accommodate up to 1 ml of sample
  • Use a quick, easy, spin-column format
  • Work on a variety of sample sources (serum, plasma, mammalian tissues or cells, plant leaves, E. coli, yeast)

*U.S. patent 6,423,666.

Ordering

Ordering

items

Use the filters below to refine results!

Image
732-6711_tn.jpg
7326711
Protein fractionation kit, includes cation exchange columns and buffers for basic protein enrichment, for 2 preps
Image
732-6710_tn.jpg
7326710
Protein fractionation kit, includes anion exchange columns and buffers for acidic protein enrichment, for 2 preps

Documents

Documents

Number Description Options
5398
Enriching Basic and Acidic Rodent Brain Proteins With Ion Exchange Spin Columns for Two-Dimensional Gel Electrophoresis, Rev A
2958
Aurum Ion Exchange Mini Kits and Columns Protocol Overview, Rev A
4110137
Instruction Manual, Aurum™ Ion Exchange Mini Kits and Columns, Rev B
2928
Aurum Ion Exchange Mini Kits and Columns Product Information Sheet, Rev A
3096
Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B