Aurum™ Ion Exchange Mini Kits

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Aurum Ion Exchange Mini Kits

Overview

Specifications
  Aurum CEX Aurum AEX
Type of support UNOsphere S UNOsphere Q
Binding Capacity 12 mg protein/column* 36 mg protein/column*
Column bed volume 0.2 ml 0.2 ml
Binding buffer 20 mM sodium acetate, pH 5.0 20 mM Tris, pH 8.3
Elution buffer Binding buffer + 1.0 M NaCl Binding buffer + 1.0 M NaCl
* Binding capacity determined using IgG (CEX) and BSA (AEX). Capacity will differ slightly depending on the protein.
Fractionation by Charge

Fractionation by charge — Chromatographic methods also can fractionate specifically or generally. Aurum ion exchange (AEX and CEX) mini columns allow selective purification of either acidic or basic proteins. Easy-to-use Aurum ion exchange kits selectively enrich either acidic or basic proteins and can be used with a variety of starting samples.

Total protein sample AEX-bound sample CEX-bound sample

Fractionation of rat brain tissue using Aurum ion exchange mini columns. Rat brain total protein extracts (3 ml) were loaded onto an Aurum AEX or CEX mini column, then eluted using the recommended protocol. The fractionated samples were treated with the ReadyPrep reduction/alkylation and 2-D cleanup kits prior to 2-D gel analysis. Left, total rat brain protein; center, sample that bound to the Aurum AEX column (acidic proteins); right, sample that bound to the Aurum CEX column (basic proteins).

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Aurum ion exchange mini kits utilize ion exchange chromatography to selectively enrich either acidic or basic proteins in less than 30 minutes. These easy-to-use kits include the columns and buffers for cation exchange (CEX) or anion exchange (AEX) chromatography and can be used with a variety of starting samples. Isolated proteins are suitable for most downstream proteomic applications, including 2-D gel analysis and mass spectrometry.

  Aurum CEX Aurum AEX
Type of support UNOsphere S UNOsphere Q
Binding Capacity 12 mg protein/column* 36 mg protein/column*
Column bed volume 0.2 ml 0.2 ml
Binding buffer 20 mM sodium acetate, pH 5.0 20 mM Tris, pH 8.3
Elution buffer Binding buffer + 1.0 M NaCl Binding buffer + 1.0 M NaCl
* Binding capacity determined using IgG (CEX) and BSA (AEX). Capacity will differ slightly depending on the protein.

Aurum ion exchange mini kits:

  • Use a patented* polymerization technology to achieve ultrahigh protein binding capacity
  • Accommodate up to 1 ml of sample
  • Use a quick, easy, spin-column format
  • Work on a variety of sample sources (serum, plasma, mammalian tissues or cells, plant leaves, E. coli, yeast)

*U.S. patent 6,423,666.

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Aurum™ CEX Mini Kit Columns

7326703
Pkg of 25, fractionation columns, cation exchange chromatography media for basic protein enrichment

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Aurum™ CEX Mini Kit

7326711
Protein fractionation kit, includes cation exchange columns and buffers for basic protein enrichment, for 2 preps

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Aurum™ CEX Mini Kit Columns (7326703)
Aurum™ AEX Mini Kit Columns

7326706
Pkg of 25, fractionation columns, anion exchange chromatography media for acidic protein enrichment

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Aurum™ AEX Mini Kit

7326710
Protein fractionation kit, includes anion exchange columns and buffers for acidic protein enrichment, for 2 preps

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Aurum™ AEX Mini Kit Columns (7326706)
Number Description Options
5398 Enriching Basic and Acidic Rodent Brain Proteins With Ion Exchange Spin Columns for Two-Dimensional Gel Electrophoresis, Rev A Click to download
2958 Aurum Ion Exchange Mini Kits and Columns Protocol Overview, Rev A Click to download
4110137 Instruction Manual, Aurum™ Ion Exchange Mini Kits and Columns, Rev B Click to download
2928 Aurum Ion Exchange Mini Kits and Columns Product Information Sheet, Rev A Click to download
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download

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