Affi-Prep Protein A Resin

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Overview

Chromatography on Affi-Prep protein A support yields highly purified immunoglobulins (IgG), selectively removes IgG from serum, or adsorbs immune complexes for antigen purification.
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Description

Description

Chromatography on Affi-Prep Protein A Resin yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgGs from mammalian species.

The Affi-Prep Protein A Resin matrix allows high linear flow rates and exhibits excellent mechanical strength. These key features allow the direct transfer of experimental conditions from the initial analytical methods development stage to the final production scale purification of product. When Affi-Prep Protein A Resin is used in conjunction with the optimized Affi-Prep Protein A MAPS II Binding Buffer, high-efficiency binding of a wide range of immunoglobulins, including all isotypes of murine IgG, is achieved.

Features and Benefits

  • Binds a wide range of monoclonal and polyclonal antibodies, including mouse IgG1
  • Macroporous polymeric support is not compressible under normal operating conditions
  • Linear flow rates up to 2,000 cm/hr are achievable
  • High flow rates at all column diameters for easy scale-up
  • Compatible with most commonly used chromatographic buffers
  • Stable pH range from 2–10
  • Multiple chromatographic cycles without loss of capacity
  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer
  • Easy to use

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Kits & Reagents

Kits & Reagents

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7324651
Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue
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7324600
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324602
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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1536159
Ready-to-use affinity media kit, includes 5 ml Affi-Gel protein A support, Affi-Gel Protein A MAPS II buffers, 1 x 10 cm Econo-Column column; for 500 mg of mouse IgG1
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7322010
Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit

Documents

Documents

Number Description Options
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
LIT230
Instruction Manual, Affi-Prep Protein A Matrix
LIT79
Instruction Manual, Affi-Prep Protein A MAPS II Kit
5712
The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A
5726
Profinia Protein A and G methods Product Information Sheet, Rev A
5701
Profinia Buffers for Protein A and Protein G Chromatography PIS, Rev B